Subtractive hybridization
Iwobi, Azuka (2003): Uncovering novel pathogenicity-associated loci among Yersinia enterocolitica species by subtractive hybridization. Dissertation, LMU München: Fakultät für Biologie
... In order to confirm that the subtracted library was enriched for tester-specific fragments, the DNA generated from the 3rd round of subtraction was probed in high stringency PCR reactions with primers specific for the ...
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Subtractive Hybridization Yields a Silver Resistance Determinant Unique to Nosocomial Pathogens in the Enterobacter cloacae Complex
... In conclusion, by subtractive hybridization we identified a sil- ver resistance determinant selectively present in a pathogenic En- terobacter strain. Furthermore, this sil operon was found in 63% of all ...
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Discrimination of Streptococcus pneumoniae from Viridans Group Streptococci by Genomic Subtractive Hybridization
... Genomic subtractive hybridization allows DNA sequences that are unique to the tester organism relative to the driver organism to be easily isolated and ...
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Suppression subtractive hybridization to investigate viruses in the lymphoid organ of Penaeus merguiensis and the gills of Cherax quadricarinatus
... environmental and sociological disturbances in land use, the ecology of the aquatic organisms and global trade patterns. A major consequence of these changes is the emergence and the spread of infectious diseases. In the ...
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Identification by Subtractive Hybridization of a Novel Insertion Element Specific for Two Widespread Burkholderia cepacia Genomovar III Strains
... using subtractive hybridization, a novel insertion element, designated IS1363, was identified in epidemic strain PHDC, known to infect many cystic fibrosis patients in the mid-Atlantic region of the United ...
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Subtractive hybridization-assisted screening and characterization of genes involved in the rice-Magnaporthe oryzae interaction
... the subtractive hybridization- assisted functional screening is an efficient approach that provides initial leads to the role of candidate genes in the complex process of plant-microbe ...
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Use of Subtractive Hybridization To Identify a Diagnostic Probe for a Cystic Fibrosis Epidemic Strain of Pseudomonas aeruginosa
... A multiresistant strain of Pseudomonas aeruginosa is widespread among cystic fibrosis (CF) patients attend- ing clinics in Liverpool, United Kingdom. Suppression subtractive hybridization was used to ...
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Comparison of RNA expression profiles on generations of Porphyra yezoensis (Rhodophyta), based on suppression subtractive hybridization (SSH)
... Subtractive hybridization is the first technique to be widely used for the purpose of identifying differentially expressed genes on a global ...suppression subtractive hybridization (SSH) ...
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Expressed genes in the lymphoid organ of broodstock banana prawn (Penaeus merguiensis) using suppression subtractive hybridization
... Most systemic viral infections in penaeid prawns are associated with the formation of lymphoid organ spheroid (LOS) cells in the lymphoid organ (LO). The development of spheroids is suggested to be a major defence ...
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Analysis and characterization of differential gene expression during rapid trophoblastic elongation in the pig using suppression subtractive hybridization
... suppression subtractive hybridization (SSH) to characterize the change in gene expression during conceptus transformation from spherical (8–9 mm) to tubular (15–40 mm) to early filamentous (>150 mm) ...
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Differential Regulation of Proteins and a Possible Role for Manganese Superoxide Dismutase in Bioluminescence of Panellus stipticus Revealed by Suppression Subtractive Hybridization
... Suppression subtractive hybridization (SSH) was employed to investigate bioluminescence in Panellus stipticus (Bull.) P. Karst. by detecting proteins differentially expressed in bioluminescent and ...
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Enhanced Expression of Interferon-Regulated Genes in the Liver of Patients with Chronic Hepatitis C Virus Infection: Detection by Suppression-Subtractive Hybridization
... Hepatitis C virus (HCV) infection causes acute and often also chronic liver disease. Worldwide, prevalence of infection is estimated to exceed that of human immunodeficiency virus infection fourfold. Because of the lack ...
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Screening and identification of specific markers for bladder transitional cell carcinoma from urine urothelial cells with suppressive subtractive hybridization and cDNA microarray
... (5’-CTAATACGACTCACTATAGGGC-3’). Subtractive hybridization was performed using PCR-Select cDNA Subtraction Kit (Clontech Laboratories, ...The subtractive library was constructed through T/A PCR ...
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Investigation of an idiopathic lesion in redclaw crayfish Cherax quadricarinatus using suppression subtractive hybridization
... In spite of the SSH transcripts that were identified in this study, there was no sequence with homology to parvovirus genes or any other viral genes. Similar results were also found in previous studies when penaeid ...
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Genomic Analysis of Lactobacillus gasseri Strains using Suppressive Subtractive Hybridization .
... The goal of this project was to use suppressive subtractive hybridization (SSH) as a way to screen the genomes of six unsequenced strains of L. gasseri for sequences absent in the sequenced neotype strain ...
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Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in virus infected cells
... suppression subtractive hybridization (SSH) coupled with cDNA microarray analysis for isolation and identification of chicken transcripts that change in expression on infection of host cells with a ...
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Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot blot hybridization
... The E. maxima Shanghai (SH) and Nantong (NT) strains were isolated from litter samples collected in commercial broiler houses in Shanghai and Nantong, China, respectively, and confirmed to be E. maxima by microscopic ...
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Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization
... The SSH approach was performed on primary granulosa cells from cultures treated or not treated by FSH. mRNA from control granulosa cells was subtracted from the mRNA from FSH treated granulosa cells and vice versa. We ...
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Differential gene expression at coral settlement and metamorphosis: a subtractive hybridization study
... to be expressed at a higher level in the settled polyp. In the case of the pool B transcripts, fifteen corresponded to differentially expressed unigenes according to the microarray study (Figure 9B). Eleven of these were ...
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Effect of probe characteristics on the subtractive hybridization efficiency of human genomic DNA
... favour hybridization kinetics and that there is a trade- off between the length of the probes and their density on the surface ...phase hybridization occurs and further support the need to optimize the cap- ...
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