SV40(RI)DNA
Study of SV40 large T antigen nucleotide specificity for DNA unwinding
... origin DNA and assembles into a hexameric/double hexameric helicase to initiate DNA replication of the viral ...fork DNA, without the help of other protein factors through coupling the en- ergy from ...
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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... the SV40 core origin and also physically interacts with T antigen, we examined whether TBP complexes might stimulate SV40 DNA replication in ...support SV40 DNA replication in vitro, ...
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Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
... We previously reported 28 that only a very small fraction of T Ag binds with a higher salt-sensitive affinity to SV40 DNA than to calf thymus DNA-cellulose under these conditions and tha[r] ...
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...(SV40) DNA has served as a useful model system for studying ...
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An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... cell-free SV40 DNA replication assays (39, 49), as a result of the replication-active under-phosphory- lated form of the protein expressed in this system ...to DNA polymerase a ...with DNA ...
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Mechanisms of interference with simian virus 40 (SV40) DNA replication by trans-dominant mutants of SV40 large T antigen.
... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ...
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Structure of Replicating Simian Virus 40 Deoxyribonucleic Acid Molecules
... We have examined SV40 RI and have observed that, during alkaline sedimentation, newly replicated SV40 DNA is not covalently attached to the parental DNA strand.. By electron microscopy o[r] ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... partially defective in untwisting the A/T track but not in melt- ing the early IP sequence at the SV40 origin of replication. Consistent with this, a deletion mutant missing the first 123 residues (124–708) has a ...
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Natural Isolates of Simian Virus 40 from Immunocompromised Monkeys Display Extensive Genetic Heterogeneity: New Implications for Polyomavirus Disease
... pathogen. SV40 DNA has recently been detected by PCR in human tumors, including pediatric and adult brain tumors (3, 21, 26), mesotheliomas (6–8, 30), and osteosarcomas (7, ...of SV40 association ...
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SV40-Immortalized Human Fibroblasts as a Source of SV40 Infectious Virions
... SV40 DNA isolated from the culture medium of immortalized fibroblasts, hereafter indicated as 45-54-2 MRC5 , was fully character- ized by PCR and DNA sequencing as previously described (25)(24) (data ...
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Infectious linear DNA sequences replicating in simian virus 40-infected cells.
... They have probably escaped esses, since both wild-type SV40 FO I DNA and detection thus far because the small linear and endo R EcoRI SV40 FO III, which were used relaxed-circular DNA co[r] ...
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Structural topography of simian virus 40 DNA replication.
... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ...
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Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
... Solid lines, pBR322 sequences; open bars, SV40 or Adl2 sequences; 0, origin of SV40 DNA replication; shaded regions, SV40 sequences containing the 5' striped regions and 3' crosshatched [r] ...
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Association of Simian Virus 40 T Antigen with Simian Virus 40 Nucleoprotein Complexes
... To determine the functional significance of the SV40 lOOK T antigen in SV40 nucleoprotein complexes containing SV40 I DNA, cells were infected with SV40 tsA58, a temperature-sensitive mu[r] ...
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Simian Virus 40 Deoxyribonucleic Acid Transcription In Vitro: Binding and Transcription Patterns with a Mammalian Ribonucleic Acid Polymerase
... It was found that i the majority of supercoiled SV40 DNA molecules displayed a single binding site for the enzyme, ii the supercoiled structure of SV40 DNA was frequently retained during[r] ...
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Efficient Transcription of a Compact Nucleoprotein Complex Isolated from Purified Simian Virus 40 Virions
... Due to the relative simplicity of simian virus 40 SV40 nucleoprotein complexes and the ability to isolate SV40 DNA, DNA-histone minichromosome, and DNA-histone viral protein core complex[r] ...
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Hyperproduction of adenovirus type 12 E1B gene product in monkey cells, using a simian virus 40 vector.
... Lanes: a and i, SV40 tsA58 DNA, form I; b and j, SV40 tsA58 DNA cleaved with HaeII, form III; c, intracellular viral DNA from SV-AdElB-B-infected cells cleaved with XbaI; d, intracellula[r] ...
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ...
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