SV40 T
Fine mapping two distinct antigenic sites on simian virus 40 (SV40) T antigen reactive with SV40-specific cytotoxic T-cell clones by using SV40 deletion mutants.
... We identified amino acids in the amino-terminal half of SV40 T antigen that are essential for the recognition of antigenic sites by these CTL clones by using H-2b cells transformed by mu[r] ...
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Evidence for Simian Virus 40 (SV40) Coding of SV40 T-Antigen and the SV40-Specific Proteins in HeLa Cells Infected with Nondefective Adenovirus Type 2-SV40 Hybrid Viruses
... Evidence for Simian Virus 40 SV40 Coding of SV40 T-Antigen and the SV40-Specific Proteins in HeLa Cells Infected with Nondefective Adenovirus Type 2-SV40 Hybrid Viruses Received for publ[r] ...
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Quantitation of CD8+ T-Lymphocyte Responses to Multiple Epitopes from Simian Virus 40 (SV40) Large T Antigen in C57BL/6 Mice Immunized with SV40, SV40 T-Antigen-Transformed Cells, or Vaccinia Virus Recombinants Expressing Full-Length T Antigen or Epitope Minigenes
... appropriate SV40 Tag-specific CTL clones and bulk Tag-specific CTL either as well as or better than did peptides corresponding to the wild-type Tag epitopes (data not ...
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Immunologic selection of simian virus 40 (SV40) T-antigen-negative tumor cells which arise by excision of early SV40 DNA.
... Indeed, when 107 such T-antigen-positive cloned cells were injected, the tumor which appeared was T antigen negative, again growing out from a very rare T-antigennegative revertant mutan[r] ...
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The Replication Protein A Binding Site in Simian Virus 40 (SV40) T Antigen and Its Role in the Initial Steps of SV40 DNA Replication
... cell-free SV40 DNA replication system and fractionated cell extracts has led to the identification and char- acterization of 10 cellular factors necessary and sufficient to reconstitute the process (5, 6, 79, 87, ...
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Simian virus 40 (SV40) T-antigen transcriptional activation mediated through the Oct/SPH region of the SV40 late promoter.
... This suggests that one function of T antigen in transcriptional activation of the late promoter is to alter factor binding at the Oct/SPH region to favor binding of factors to the SPH si[r] ...
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... large T antigen that specifically interacts with the SV40 origin has been purified and its binding to DNA has been ...purified T antigen DNA-binding domain for the SV40 origin is comparable to ...
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Simian virus 40 (SV40) T-antigen mutations in tumorigenic transformation of SV40-immortalized human uroepithelial cells.
... We observed a very low efficiency of transformation to G418' by pSV2Neo in SV-HUC and CK/ SV-HUC2 compared with T-SV-HUC, the chemically transformed tumorigenic derivatives of SV-HUC, an[r] ...
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Extraction and fingerprint analysis of simian virus 40 large and small T-antigens.
... Anti-T serum was produced by injecting SV40-transformed hamster cells Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A study of simian virus 40 SV40 T-antigens isolate[r] ...
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An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... of T antigen could be functionally significant is in agreement with the observations that some mutations in the coding sequences of the first exon of T antigen caused defective viral SV40 DNA ...
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Differential induction of cytolytic susceptibility by E1A, myc, and ras oncogenes in immortalized cells.
... For example, SV40 T-antigen-expressing, transformed mouse cell lines are susceptible to lysis by NK cells and activated macrophages, whereas SV40 T-antigen-expressing transformed hamster[r] ...
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Initiation of simian virus 40 DNA replication in vitro.
... Since SV40 DNA replication absolutely depends on SV40 T antigen, anti-T serum from a tumor-bearing hamster was added to the reaction mixture containing both HeLa nuclear extract and CosI[r] ...
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DNA-mediated gene transfer in Friend leukemia cells by cotransfection of simian virus 40 DNA with herpes simplex virus thymidine kinase DNA.
... Expression of SV40 T-antigen in FLCs after virus infection, and expression of SV40 T-antigen and HSV-TK gene after cotransfection with calcium phosphate-DNA precipitates in Friend leukem[r] ...
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Properties of simian virus 40 small t antigen overproduced in bacteria.
... To detect SV40-related proteins expressed in bacteria and compare them with authentic SV40 small t antigen, both SV40-infected monkey cells and HP1 bacterial cells were pulse-labeled wit[r] ...
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SV40-Immortalized Human Fibroblasts as a Source of SV40 Infectious Virions
... other SV40 strains did not occur. The amplification pattern of different SV40 DNA regions present in immortalized fibroblasts is indistinguish- able from that of the input SV40 strain, indicating ...
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Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.
... Moreover, SF9 T had ATPase activity comparable to that of T produced in monkey cells, exhibited helicase activity and SV40 origin-specific DNA binding, and was active in the SV40 DNA rep[r] ...
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Structural relationship between the 100,000- and 17,000- molecular-weight T antigens of simian virus 40 (SV40) as deduced by comparison with the SV40-specific proteins coded by the nondefective adenovirus type 2-SV40 hybrid viruses.
... Our results confirm that the 100K and 17K T antigens share a common N-terminal region coded between the initiator AUG triplet at 0.65 map units 13 and 0.59 map units, whereas none of the[r] ...
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Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.
... A mutant T antigen carrying alanine in place of Thr-124 T124A also failed to replicate SV40 DNA in vitro and was defective in its ability to initiate replication in vitro Knowledge of eu[r] ...
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Restricted Protein Phosphatase 2A Targeting by Merkel Cell Polyomavirus Small T Antigen
... small T (sT) ...and SV40 sT. While SV40 sT coimmunopurified with subunits PP2A A ␣ and PP2A C, MCV sT coimmunopurified with PP2A A ␣ , PP2A A  , and PP2A ...large T stabilization domain (LSD) ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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