SV40 virus large T antigen
Expression of SV40 virus large T antigen by recombinant adenoviruses activates proliferation of corneal endothelium in vitro
... Ad5-SVR4 virus was used to introduce the large T antigen encoding region of the SV40 virus into bovine and human corneal endothelial ...of large T antigen ...
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...
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Glial Cell-Specific Regulation of the JC Virus Early Promoter by Large T Antigen
... JC virus large T-antigen gene fused to the firefly luciferase gene ...JC virus were generated in the context of the 408-bp upstream sequence, using a QuickChange PCR-based site-directed ...
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Mutual functional antagonism of the simian virus 40 T antigen and the hepatitis B virus trans activator.
... Positive and negative regulation of transcription in vitro: enhancer binding protein AP-2 is inhibited by SV40 T antigen.. Production of simian virus 40 large tumor antigen in bacteria: [r] ...
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Site-directed mutagenesis of the simian virus 40 large T-antigen gene: replication-defective amino acid substitution mutants that retain the ability to induce morphological transformation.
... We report here the isolation and characterization of in vitro-constructed amino acid substitu- The large tumor antigen T antigen of simian virus 40 SV40 is a regulatory protein that is e[r] ...
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Only a minor fraction of plasma membrane-associated large T antigen in simian virus 40-transformed mouse tumor cells (mKSA) is exposed on the cell surface.
... 3926 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The bulk of simian virus 40 SV40 large T antigen in SV40-infected and -transformed cells localizes within the cell [r] ...
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Nucleotide sequence changes in polyoma virus A gene mutants.
... The sequence in the polyoma large T-antigen surrounding the site of the ts-a change Ala-GlyVal-Ala-Trp is conserved between the T-antigens of polyoma virus, simian virus 40 SV40, and BK [r] ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding.
... A mutant T antigen carrying alanine in place of Thr-124 T124A also failed to replicate SV40 DNA in vitro and was defective in its ability to initiate replication in vitro Knowledge of eu[r] ...
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Phenotype-specific phosphorylation of simian virus 40 tsA mutant large T antigens in tsA N-type and A-type transformants.
... 4414 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest To identify molecular differences between simian virus 40 SV40 tsA58 mutant large tumor antigen large T in cells of[r] ...
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A DNA replication-positive mutant of simian virus 40 that is defective for transformation and the production of infectious virions.
... The large tumor antigen T antigen of simian virus 40 SV40 is a multifunctional regulatory protein that is required for a number of steps in productive viral infection, including DNA repl[r] ...
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Association of p53 binding and immortalization of primary C57BL/6 mouse embryo fibroblasts by using simian virus 40 T-antigen mutants bearing internal overlapping deletion mutations.
... Mutants with changes within or near a hydrophobic region of simian virus 40 large tumor antigen are defective for binding cellular protein p53.. The replication functions of SV40 T antig[r] ...
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... the SV40 large-T antigen might be me- diated by the mRNA that encodes the large-T antigen, rather than by the ...mutant large-T plasmid (pR137) was ...
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...replication, T antigen must interact with three ...
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Study of SV40 large T antigen nucleotide specificity for DNA unwinding
... Simian Virus 40 (SV40) Large Tumor Antigen (LT) is an essential enzyme that plays a vital role in viral DNA replication in mammalian ...the SV40 origin to initiate genomic ...
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Free and viral chromosome-bound simian virus 40 T antigen: changes in reactivity of specific antigenic determinants during lytic infection.
... 635 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Simian virus 40 SV40 large T antigen TAg, both free and bound to mature 70S and replicating 90S SV40 chromosomes, wa[r] ...
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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... for T antigen: impli- cations for viral ...to T antigen antagonizes viral DNA replica- tion in vitro ...between T-antigen hexamers in unwinding viral DNA ...on T ...
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Quantitation of CD8+ T-Lymphocyte Responses to Multiple Epitopes from Simian Virus 40 (SV40) Large T Antigen in C57BL/6 Mice Immunized with SV40, SV40 T-Antigen-Transformed Cells, or Vaccinia Virus Recombinants Expressing Full-Length T Antigen or Epitope Minigenes
... simplex virus type 1 (HSV-1) gB peptide (SSIEF ARL) (5, 7, 23, 27, 34, 41, 42, 50, ...appropriate SV40 Tag-specific CTL clones and bulk Tag-specific CTL either as well as or better than did peptides ...
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Differential induction of structural changes in the simian virus 40 origin of replication by T antigen.
... 1228 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The ATP-dependent binding of the simian virus 40 SV40 large tumor antigen T antigen to the SV40 origin of replicati[r] ...
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Replication of simian virus 40 origin-containing DNA during infection with a recombinant Autographa californica multiple nuclear polyhedrosis virus expressing large T antigen.
... simian virus 40 (SV40) has been extensively characterized. The virus possesses just one origin and encodes only a single protein involved in the replication of its DNA, the large T ...
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