SYBR Green quantitative PCR
Detection of Duck Hepatitis B Virus DNA on Filter Paper by PCR and SYBR Green Dye Based Quantitative PCR
... able diagnosis and analysis of DNA isolated from serum dried on filter papers (3, 6). In this study, a sensitive PCR assay was applied to detect DHBV from dried serum or blood on filters. The immobilization ...
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Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green Based Quantitative PCR Assay
... rapid SYBR green quantitative PCR (qPCR) assay that can identify ...the SYBR green qPCR assay further by incorporating a DNA extraction procedure for skin swabs and by in- ...
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The development of a rapid SYBR Green I based quantitative PCR for detection of Duck circovirus
... a quantitative real-time PCR for the detection of DuCV based on SYBR Green I dye was ...real-time PCR for diagnosing and monitoring ducks with DuCV infection, we compared the results of ...
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Development of a duplex SYBR Green I based quantitative real time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
... duplex SYBR Green I-based quantitative real-time PCR assay was developed for the simultaneous detection and differentiation of GPV and ...
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SYBR Green Based Real Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False Negative Results Due to Strain Variability
... Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus ...dye SYBR green. The SYBR green-based assay was as ...
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Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... Real-time PCR oligonucleotide primers were manually designed for each of the genes to assure maximal effi- ciency and sensitivity, according to the following parame- ters: avoidance of the formation of self and ...
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Detection and Quantification of Infectious Hypodermal and Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real Time Quantitative PCR and SYBR Green Chemistry
... real-time PCR detection and quantification method for infectious hypodermal and hematopoietic necrosis virus (IHHNV), a single-stranded DNA virus, and white spot virus (WSV), a double-stranded DNA (dsDNA) virus ...
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Quantitative real time PCR with SYBR Green detection to assess gene duplication in insects: study of gene dosage in Drosophila melanogaster (Diptera) and in Ostrinia nubilalis (Lepidoptera)
... agent SYBR Green I dye and the sequence-specific TaqMan ...[11]. SYBR Green I dye detection simplifies the experimental design and reduces the assay costs (there is no need for specific ...
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Pcr Taqman Protocol Vs Sybr Green Protocol
... on pcr taqman protocol vs sybr green protocol and detection of mycobacteria present in ...in PCR product ...therefore, pcr taqman protocol vs sybr green protocol, being ...
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SYTO dyes and EvaGreen outperform SYBR Green in real time PCR
... D: SYBR Green-based real-time quantitative PCR assay for detection of West Nile virus circumvents false-negative results due to strain ...real-time PCR: effects of dye concentration and ...
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Brilliant III Ultra-Fast SYBR Green QRT-PCR Master Mix
... a PCR reaction is monitored in real-time, the results can be displayed as an amplification plot (see Figure 1), which reflects the change in fluorescence during ...background. Quantitative information based ...
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Development and Application of a Universal Hemoplasma Screening Assay Based on the SYBR Green PCR Principle
... universal SYBR green PCR assay described here was designed as a quantitative, closed-tube method for inexpensive and rapid screening of samples for hemotropic Mycoplasma ...hemoplasma ...
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Sybr Green Qpcr Protocol Applied Biosystems
... successful quantitative analysis of different time quantitative pcr methods for qpcr protocol may offer a given ...assay. PCR continues and is measured at row end with each PCR ...your ...
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Real time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
... ABSTRACT: Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected ...dye ...
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Real-time PCR for quantitation of bovine viral diarrhea virus RNA using SYBR Green I fluorimetry
... ABSTRACT: Quantitative real-time RT-PCR (qRT-PCR) assay was developed for the detection and quantification of bovine viral diarrhea virus (BVDV) in clinical samples from persistently infected ...dye ...
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In-silico Designing of SYBR Green Based Real-Time PCR Array for the Quantification of Salmonellae and Enterotoxigenic Escherichia coli in Water
... real-time PCR array for culture-independent quantitative enumeration of Salmonellae and ETEC have the potential for improving specificity and minimizing time and cost needed for in vitro verification of ...
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Establishment of one step SYBR green based real time PCR assay for rapid detection and quantification of chikungunya virus infection
... the SYBR green I-based RT-PCR for the quantitation and detection of CHIKV is ...decreases quantitative reliability. The SYBR Green-based RT-PCR assay is known to be less ...
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Quantitative PCR Systems
... FullVelocity ™ QPCR and QRT-PCR Reagents b FullVelocity ™ QPCR Master Mixes provide a fast and economical system for real-time QPCR applications. FullVelocity master mix results are produced 30 to 50% faster than ...
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Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... RNA standards in this study were generated using BVDV RNA in cell cultures by in vitro transcription with T7 RNA polymerase for quantifying BVDV RNA by real-time RT-PCR. A RNA standard curve with a lin- ear range ...
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SYBR Green PCR Master Mix and SYBR Green RT-PCR Reagents Kit
... in PCR and you use the method described below, AmpErase ® UNG treatment can prevent the reamplification of carryover PCR products in subsequent ...degrade PCR products from previous PCR ...
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