T7 RNA Polymerase
Varicella-zoster virus glycoprotein gpI/gpIV receptor: expression, complex formation, and antigenicity within the vaccinia virus-T7 RNA polymerase transfection system.
... HeLa cells were infected with recombinant vaccinia virus expressing the bacteriophage T7 RNA polymerase and then transfected by lipofection with pTM1-gpI, pTM1-gpIV, or pTM1 vector only.[r] ...
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Decreased gene expression from T7 promoters may be due to impaired production of active T7 RNA polymerase
... active T7 RNA polymerase, in turn decreasing expression from the ...diminished T7 RNA polymerase expression could be sufficient to abolish pro- tein production from all resident ...
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Evidence for direct involvement of T7 RNA polymerase bacteriophage DNA replication.
... The experiments scripts are used as primers for DNA synthesis indicated that the inactivation of the gene 1 18, 19, In other cases this has not yet been RNA polymerase caused the cessati[r] ...
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Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor.
... FUERST1* Department of Molecular Genetics, MedImmune Inc., Gaithersburg, Maryland 20878,1 and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesd[r] ...
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Virus-specific cytotoxic T-lymphocyte activity elicited by coimmunization with human immunodeficiency virus type 1 genes regulated by the bacteriophage T7 promoter and T7 RNA polymerase protein.
... the T7 RNAP protein, and immunized 2 and 18 h later with the pTMIgp120 plasmid, which encodes the HIV-1 gp120 protein under the control of the T7 promoter, were also eval- uated for envelope-specific CTL ...
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Novel hybrid inhibitors of the phage T7 RNA polymerase: synthesis, docking and screening in vitro
... phage T7 DNAdependent RNA polymerase three effective inhibitors of the RNA syntheses with the IC 50 value of ...Т7 RNA polymerase transcription ...
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A Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus with Efficient Gene Transfer and Expression
... defective RNA genome. This is the first report on constructing a replicon-based RNA vector in the family Paramyxoviridae which replicates in infected cells but does not infect neighboring ...expressing ...
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Recovery of Infectious Pariacoto Virus from cDNA Clones and Identification of Susceptible Cell Lines
... genomic RNA is icosahedrally ordered, with the RNA forming a dodecahedral cage of 25-nucleotide (nt) duplexes that underlie the inner surface of the ...the RNA-dependent RNA polymerase, ...
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The RING Domain and the L79 Residue of Z Protein Are Involved in both the Rescue of Nucleocapsids and the Incorporation of Glycoproteins into Infectious Chimeric Arenavirus-Like Particles
... full-cycle RNA rep- lication and transcription and that Z protein is a powerful inhibitor of these processes ...the T7 promoter and the internal ribosome entry site of encepha- lomyocarditis virus into the ...
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Synechococcus elongatus as a model of photosynthetic bioreactor for expression of recombinant β-glucosidases
... dedicated T7 RNA polymerase for the expression of the gene of interest under the control of a nickel-inducible ...induced T7 RNA polymerase expression which, in turn, induced ...
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Recovery of NV Knockout Infectious Hematopoietic Necrosis Virus Expressing Foreign Genes
... the T7 RNA polymerase promoter and the autocatalytic hepatitis delta virus ...the T7 RNA polymerase (vTF7-3) and cotransfection of pIHNV-Pst together with plasmids encoding the ...
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Rescue of Mumps Virus from cDNA
... infected-cell RNA by RT-PCR using primer pairs which contained unique restriction sites; in each case the positive sense primer contained a 5⬘-proximal NotI site in addition to the virus-specific endo- nuclease ...
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Hepatitis C virus in vitro replication is efficiently inhibited by acridone Fac4
... Figure 3. Fac4 possible mechanisms of action: A) Intercalation assay, evaluating Fac4 dsRNA intercalation property. Doxorrubicin (Doxo) was used as positive control. B) Evaluation of Fac4 effect over T7 RNA ...
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T7 Protein Synthesis in F′ Episome-Containing Cells: Assignment of Specific Proteins to Three Translational Groups
... T7 infection of cells containing the mutant F' PIF-2A episome led to normal synthesis of T7 RNA polymerase and T7 DNA polymerase; T7 lysozyme was synthesized at 30% of the maximal level [r] ...
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The role of vesicular stomatitis virus matrix protein in inhibition of host-directed gene expression is genetically separable from its function in virus assembly.
... BHK cells were infected with vTF7.3, a vaccinia virus recombinant expressing the T7 RNA polymerase, and then transfected with T7 expression plasmids encoding either MN1 M protein lane 1,[r] ...
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Three of the Four Nucleocapsid Proteins of Marburg Virus, NP, VP35, and L, Are Sufficient To Mediate Replication and Transcription of Marburg Virus-Specific Monocistronic Minigenomes
... Then, RNA was fixed for 3 min by UV ...the T7 RNA polymerase promoter and the hepatitis delta virus ribozyme ...the T7 RNA polymerase promoter, 668 nt of the CAT gene in a ...
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Crimean-Congo Hemorrhagic Fever Virus-Encoded Ovarian Tumor Protease Activity Is Dispensable for Virus RNA Polymerase Function
... the T7/ribozyme system was a suitable alternative to the rather limited CCHFV PolI S segment minigenome system previously developed ...of T7 cS, cM, and cL minigenome plasmids into BSR-T7/5 cells ...
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Efficient cDNA-Based Rescue of La Crosse Bunyaviruses Expressing or Lacking the Nonstructural Protein NSs
... negative-sense RNA genome which consists of the three segments L, M, and ...viral RNA segments in the antigenomic orientation, transcribed intracellularly by the T7 RNA polymerase and ...
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Localization of antigenic sites of the S glycoprotein of feline infectious peritonitis virus involved in neutralization and antibody-dependent enhancement.
... the T7 promoter and part of T7 gene 10 followed by a polylinker ...with T7 RNA polymerase resulted in the production of fusion proteins containing 260 amino acids of the T7 gene ...
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Expression, processing, and assembly of foot-and-mouth disease virus capsid structures in heterologous systems: induction of a neutralizing antibody response in guinea pigs.
... Synthesis of foot-and-mouth disease virus capsid proteins in insect cells using baculovirus expression vectors.. T7 RNA polymerase can direct expression of influenza virus cap-binding pr[r] ...
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