Template switching
Correlated Template-Switching Events during Minus-Strand DNA Synthesis: a Mechanism for High Negative Interference during Retroviral Recombination
... line, template-switching event; ppt/PPT, poly- purine ...a template for DNA synthesis until a break in the RNA is ...a template for DNA ...
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RNA-RNA recombination in Sindbis virus: roles of the 3' conserved motif, poly(A) tail, and nonviral sequences of template RNAs in polymerase recognition and template switching.
... undergo template switching in vivo ...and template switching; (ii) a full-length SIN RNA carrying the 3 * -terminal 24 nt but lacking the poly(A) tail is noninfectious; (iii) SIN RNAs which ...
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Effects of Limiting Homology at the Site of Intermolecular Recombinogenic Template Switching during Moloney Murine Leukemia Virus Replication
... strong-stop switching (40, 49) and during template switching in purified reaction mixtures (35, ...acceptor template, followed by the sequence of the ITR of that acceptor Nucleotides boxed in ...
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Human Immunodeficiency Virus Type 1 Genetic Recombination Is More Frequent Than That of Moloney Murine Leukemia Virus despite Similar Template Switching Rates
... Two template switching events were required to generate functional ...acceptor template (2,633 bases of the sequence between the LTRs) was the recombination ...and switching could occur at ...
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Recombination Events in Neurospora crassa May Cross a Translocation Breakpoint by a Template-Switching Mechanism
... that template switching occurs in some recombination events, with repair synthesis alternating between use of the homolog and the initiating chromatid as ...
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Effect of Distance between Homologous Sequences and 3′ Homology on the Frequency of Retroviral Reverse Transcriptase Template Switching
... of template switching was greater within the 5 ⴕ regions in comparison to the 3 ⴕ ...RT switching templates within the 5 ⴕ regions doubled when the MLV packaging sequence ( ⌿ ) was placed between the ...
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Template Switching During Break-Induced Replication Is Promoted by the Mph1 Helicase in Saccharomyces cerevisiae
... of switching between donor templates during BIR by genetic selection in diploid ...of template switch products, we found a high frequency of recombination between chromosome homologs during BIR, suggesting ...
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Structural Determinants of Murine Leukemia Virus Reverse Transcriptase That Affect the Frequency of Template Switching
... RNA template and reassociation of the DNA at another region of homology ...these template-switching events, minus-strand DNA transfer, involves the transfer of the minus- strand strong-stop DNA from ...
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Dimerization and Template Switching in the 5′ Untranslated Region between Various Subtypes of Human Immunodeficiency Virus Type 1
... intersubtype template switching. A combined dimerization-template-switching assay was devel- oped with donor and acceptor templates sharing a region of homology of approximately 170 ...
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Base Pairing between cis-Acting Sequences Contributes to Template Switching during Plus-Strand DNA Synthesis in Human Hepatitis B Virus
... two template switches (primer translocation and circularization) during synthesis of plus-strand DNA to generate a relaxed-circular (RC) DNA ...both template switches through base pairing, 3E with the 3 ⴕ ...
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Relative Rates of Retroviral Reverse Transcriptase Template Switching during RNA- and DNA-Dependent DNA Synthesis
... plate switching events occurring during transfection, RNA- dependent DNA synthesis, and DNA-dependent DNA ...RT template switching events that resulted in direct repeat dele- tions of RB-LLP was ...
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Stimulation of Replication Template-Switching by DNA-Protein Crosslinks
... Many mutations arise during by improper base selection by DNA polymerases or by DNA lesions that promote miscoding. Templated-mutations, such as those at QP sites, are fundamentally different in nature because they are ...
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Coronavirus Nucleocapsid Protein Facilitates Template Switching and Is Required for Efficient Transcription
... vitro template switching ...in template switch- ...vitro template switching assay. (A) HIV-derived template switching ...chaperone, template switching occurs ...
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Template switching by reverse transcriptase during DNA synthesis.
... We examined several variables that affected the efficiency of the reaction: i the reaction time, ii the relative amounts of acceptor and donor template, iii the extent of sequence overla[r] ...
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Interchromosomal template-switching as a novel molecular mechanism for imprinting perturbations associated with Temple syndrome
... aCGH: Array comparative genomic hybridization; AOH: Absence of heterozygosity; BIR: Break-induced replication; CADD: Combined annotation- dependent depletion; CGRs: Complex genomic rearr[r] ...
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Structure-Based Moloney Murine Leukemia Virus Reverse Transcriptase Mutants with Altered Intracellular Direct-Repeat Deletion Frequencies
... this template than on the 117-base repeat, but some mutants (K53L, R120L, D124A/E201G, and K53L/K102A) displayed consistent ...in template switching compared to the wild ...in template switch- ...
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Different De Novo Initiation Strategies Are Used by Influenza Virus RNA Polymerase on Its cRNA and Viral RNA Promoters during Viral RNA Replication
... cRNA template (cis- ...cRNA template could dissociate and realign to nucleotides 1 and 2 of a vRNA template for extension ...vitro template-switching experiment in which a cRNA pro- ...
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Homologous recombination occurs in a distinct retroviral subpopulation and exhibits high negative interference.
... genomic template RNAs and frequently switches templates between ...intermolecular template switching. The rate of intermolecular template switching within the subpopulation of viruses ...
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Integration of Hepadnavirus DNA in Infected Liver: Evidence for a Linear Precursor
... of template switching during the nested PCR, we used as a template a dimer-containing plasmid DNA that had been cut at multiple sites with Sau3AI and at the unique site with ...Intramolecular ...
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Human endogenous retroviral elements promote genome instability via non-allelic homologous recombination
... aCGH: array comparative genomic hybridization; BCM: Baylor College of Medicine; bp: base pair; CNV: copy-number variation; FoSTeS: fork stalling and template switching; HERV: human endog[r] ...
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