The development of high-throughput sequencing
High-Throughput Sequencing Reveals miRNAs Affecting Follicle Development in Chicken
... growth, development. In this study, we used next generation sequencing technology sequenced miRNAs of the hair follicle derived from the 13 day-old chicken (Gallus gallus) embryos in which from Kirin ...
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Establishment and Development of Oral Microflora in Month-Old Toddlers Monitored by High-Throughput Sequencing
... in toddlers is accompanied by high dynamism in oral microbial communities. The Alpha diversity index showed that toddlers have greater microflora species diversity at 18 and 24 months of age than at 12 months of ...
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Standards for Sequencing Viral Genomes in the Era of High Throughput Sequencing
... vaccine development or the testing of therapeutics, making it imperative to monitor the purity of viral ...HT sequencing provides a powerful method for not only detecting the presence of contaminants within ...
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High-Throughput Sequencing and Metagenomic Data Analysis
... the development of more efficient sequencing approaches, the metagenomic workflow became ...generation sequencing approaches, it is not required to conduct sample ...
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Functional Interpretation of High-Throughput Sequencing Data.
... "Vascular development", the most significant GO term based on differential expression, was also significantly enriched for GRα binding using the nearest TSS analysis ...
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High throughput sequencing to decipher the genetic heterogeneity of deafness
... child development, education, social inte- gra tion, and the quality of life of the aff ected individual, with a substantial impact on public ...is high preva lence, combined with the striking genetic ...
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Validation of high throughput sequencing and microbial forensics applications
... High throughput sequencing (HTS) generates large amounts of high quality sequence data for microbial ...the development and implementation of microbial forensics methods used for ...
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High-throughput sequencing of cytosine methylation in plant DNA
... of high- throughput sequencing data and specifically that pertain- ing to the methylome, there exist some clear areas for ...The development of tools for the methylome such as RSEM [81] or ...
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Bayesian modelling of high-throughput sequencing assays with malacoda.
... function. High-throughput screens powered by NGS have greatly increased the rate of vari- ant functionalization, but the development of comprehensive statistical methods to analyze screen data has ...
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The Role of High Performance, Grid and Cloud Computing in High-Throughput Sequencing
... All platforms have their strengths and weaknesses, which must be considered to enable appropriate use of sequencing data [3]. Different HTS technologies apply different protocols, which in turn determine the type ...
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Identification of microRNAs and their targets in Finger millet by high throughput sequencing
... MicroRNAs are short non-coding RNAs which play an important role in regulating gene expression by mRNA cleavage or by translational repression. The majority of identified miRNAs were evolutionarily conserved; however, ...
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Computational approaches for metagenomic analysis of high-throughput sequencing data
... The methods and programs described in this thesis would not have existed without my industrial supervisor, Come Raczy. His insight to use k -mers and a sorted list to find alignments for assembly provided the first step ...
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Applications of High-Throughput Sequencing Data Analysis in Transcriptional Studies
... tance traits. Traditional QTL analysis and Genome-wide Association Studies (GWAS) have led to reports of dozens of QTL or markers on almost every ovine chromosome that are associated with various resistance phenotypes, ...
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Probabilistic methods for quality improvement in high-throughput sequencing data
... the development of a wide range of so-called next-generation sequencing (NGS) technologies, that are capable of generating millions of sequences at only a tiny fraction of cost than a decade ago [van Dijk ...
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High-throughput DNA sequencing laboratories
... our development efforts have centered on overcoming the undesirable as- pects of either matrix ...700 high-quality ...the sequencing reaction fragments for ...a high percentage of failure due ...
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Empirical assessment of sequencing errors for high throughput pyrosequencing data
... High-throughput pyrosequencing error characteristics have also been studied with the aim of producing more realistic simulators for this kind of data. Balzer et al. [11] analysed a data set of bacterial ...
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Indices and Applications in High-Throughput Sequencing
... Bentley, D. R., Balasubramanian, S., Swerdlow, H. P., Smith, G. P., Milton, J., Brown, C. G., Hall, K. P., Evers, D. J., Barnes, C. L., Bignell, H. R., Boutell, J. M., Bryant, J., Carter, R. J., Keira Cheetham, R., Cox, ...
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Improving quality of high-throughput sequencing reads
... [33] N. Beerenwinkel and O. Zagordi, “Ultra-deep sequencing for the anal- ysis of viral populations,” Current Opinion in Virology, vol. 1, no. 5, pp. 413–418, 2011. [34] R. Durbin, D. Altshuler, R. Durbin, G. ...
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Universal Count Correction for High-Throughput Sequencing
... Discussion We have shown that per-base count overdispersion is a widespread and consistent phenomenon in high-throughput sequencing experiments. While correcting for exon-level over- dispersion has ...
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High-throughput sequencing of the chicken gut microbiome
... Comparison of the results in Chapter Three and Chapter Four was not always possible for all organs due to the depth of coverage gained in the V3-V4 16S rRNA gene-fragment sequencing. Furthermore, because the ...
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