trypan blue dye exclusion
![Comparative in vitro cytotoxic studies of novel 8-(4'/2'-Methoxy/Unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonane derivatives on ehrlich ascites carcinoma cell line](data:image/gif;base64,R0lGODlhAQABAIAAAP///wAAACH5BAEAAAAALAAAAAABAAEAAAICRAEAOw==)
Comparative in vitro cytotoxic studies of novel 8-(4'/2'-Methoxy/Unsubstituted phenylcarbamoyl)bicyclo[3.3.1]nonane derivatives on ehrlich ascites carcinoma cell line
... vitro cytotoxic studies were performed using Ehrlich Ascites Carcinoma cell line by Trypan blue dye exclusion assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cell ...
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In vitro Cytotoxic Activity of Orthosiphon thymiflorus Roth ) Sleensen Leaf Extract against Dalton Lymphoma Ascites Cell Line
... using Trypan blue dye exclusion technique MTT assay was used to evaluate the reduction of viability of cell cultures in the presence and absence of the ...
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Journal of Applied Pharmaceutical Science
... The purpose of present study was to investigate the methanolic bark extract of Hymenodictyon excelsum (Roxb) Wall for its apoptosis, cytotoxic activities. Induction of apoptosis was carried out on L-929 cell line. The ...
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Assessment of Cytotoxicity of Dimethyl Sulfoxide in Human Hematopoietic Tumor Cell Lines
... Methods: Human leukemic T cells (Molt-4 and Jurkat) and monocytes (U937 and THP1) were cultured in complete RPMI mediums. The cells at different logarithmic growth phases were incubated with different concentrations of ...
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Evaluation of anticancer compounds from suspension cultures of Holy Basil (Ocimum sanctum L.)
... The anticancer activity of compounds extracted from cell suspension cultures of O. sanctum was evaluated by MTT assay and Trypan blue dye exclusion test. The MTT assay is based on the cleavage ...
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A comparison between cytotoxicity induced by two resin based sealers (2Seal and AH Plus) in Saos-2 and MG-63 cell lines
... sealers extracts using MTT assay (20) and Trypan blue dye exclusion (TB) method (21). Briefly, after washing the cells with D-PBSA, 200µl of MTT (Sigma-Aldrich, UK) solution in PBS (5 mg/ml) ...
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Jasplakinolide Induces Apoptosis in Various Transformed Cell Lines by a Caspase-3-Like Protease-Dependent Pathway
... FIG. 5. Comparison of sensitivities to jasplakinolide-induced cell death of various murine cell lines. Murine T-cell lymphoma EL-4 cells (a), myeloma SP-2/0 cells (b), macrophage-like J774.1 cells (c), or fibroblast L ...
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Synthesis and in-vitro cytotoxic screening of triethylammonium{[7-hydroxy-4,9-dinitro-8-(phenylcarbamoyl)bicyclo[3.3.1]nona-3,7-dien-2-ylidene(oxido)-λ[..]
... The title compound (TBO) with bicyclo[3.3.1]nonane skeleton was synthesized in an efficient one pot procedure employing a green methodology. The compound was structurally confirmed by UV-Visible, FT-IR, NMR and single ...
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IN VITRO SCREEENING OF PHYTOCHEMICALS AND ANTICANCER ACTIVITY OF ARGEMONE MEXICANA LEAF EXTRACT
... The cytotoxic effect aqueous extract of A. mexicana leaf on human breast cancer cell line MCF-7 was investigated for their viability with trypan blue dye exclusion method. The cell viability ...
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In vitro antioxidant and cytotoxicity studies of Curcuma amada Roxb. (Mango ginger)
... by Trypan blue dye exclusion method and MTT assay using two different cell lines such as Dalton Lyphoma Ascites (DLA) and Human Cervical Cancer Cell Line ...
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Mitochondrial Impairment Induced by Sub-Chronic Exposure to Multi-Walled Carbon Nanotubes
... Based on data previously obtained using the same cell model and from the literature regarding possible exposures in the workplace [6, 17, 18], we assessed the effects of sub-chronic exposure to 2 µg mL -1 of CNTs ...
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EVALUATION OF THE INVITRO ANTI CANCER POTENTIAL OF ANOGEISSUS ACUMINATE
... Trypan Blue is a blue acid dye that has two azo chromophore ...groups. Trypan Blue is an essential dye, used in estimating the number of viable/dead cells present in a ...
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Trypan Blue Exclusion Assay Verifies in Vitro Cytotoxicity of New Cis-Platinum (II) Complex in Human Cells
... of trypan blue dye, and then incubated for 3 minutes at 37 o ...with trypan blue ...the dye. Some of the cell suspension was placed with a pipette and the trypan ...
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Adverse effects of antipsychotics on micro vascular endothelial cells of the human blood–brain barrier
... Evans blue occurs via an energy-dependent transcytosic process (Abbott 2013), its decrease with the lower doses of CLZ and CPZ is consistent with the ability of these drugs to decrease the energy state of the ...
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Ovarian Follicle Disaggregation to Assess Granulosa Cell Viability
... Background: Mammalian ovaries contain follicles containing an oocyte en- closed by layers of granulosa cells (GC). Follicle growth and oocyte matura- tion are largely dependent on GC numbers and viability, but there is ...
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Preparation of Pre Confluent Retinal Cells Increases Graft Viability In Vitro and In Vivo: A Mouse Model
... We sought to ascertain if culturing DH01 cells post-confluence or the process of preparing cells for transplantation (TC) induced cell death, and more specifically apoptosis. We cultured DH01 cells to pre-confluent (0.56 ...
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Study of in Vitro Cytotoxicity and in Vivo Anti- Tumour and Anti-Inflammatory Activities of Gmelina Arborea Roxb. Stem Bark
... stem bark Pharmacological studies: In vitro cytotoxic assay using DLA, EAC cell lines by Trypan blue exclusion method In vivo anti inflammatory activity of plant extract by a Dextran i[r] ...
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In vitro study on anti-cancer properties of genistein in tongue cancer
... While PE/CA-PJ15, PE/CA-PJ49, HSC-3 cells were treated with genistein at the same concentrations and time points, Trypan blue exclusion method was used to validate the cell viabi[r] ...
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Original Article Regulation of exogenous P53 combined with Dickkopf-1 on human osteosarcoma MG-63 cells
... by trypan blue exclusion and flow cytometry; the expres- sion levels of tumor suppressor gene P53 and oncogene c-fos and c-myc were detected by real-time fluorescent quantitative and western blot in ...
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Radio-Sensitization of Human Leukaemic MOLT-4 Cells by DNA-Dependent Protein Kinase Inhibitor, NU7026
... we determined the percentage of cells in g1, s, and g2 phase using flow-cytometric analysis of Dna content. we measured cell cycle distribution 24 and 72 hours after irra- diation. cells in the early phase of apoptosis ...
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