TUNEL staining
Protective effect of geranylgeranylacetone, an inducer of heat shock protein 70, against drug-induced lung injury/fibrosis in an animal model
... lungs, TUNEL staining was performed for the lungs at BLM day1 and ...day3. TUNEL staining showed that TUNEL-positive cells were observed mainly in epithelial cells of BLM-treated lungs ...
11
Download Download PDF
... uorescent staining demonstrated intense positive reactions for HSP70 (Figure1A) and αBC (Figure1C) in the substantia nigra of rats from the treatment groups; however, no immunopositive cells were detected in the ...
9
Early Stage of Establishment of Persistent Sendai Virus Infection: Unstable Dynamic Phase and Then Selection of Viruses Which Are Tightly Cell Associated, Temperature Sensitive, and Capable of Establishing Persistent Infection
... positive TUNEL staining, while cells infected with cloned viruses capable of establishing persistent infection showed weak TUNEL staining, suggesting that a loss or a reduction of ...
13
Cure of prediabetic mice by viral infections involves lymphocyte recruitment along an IP 10 gradient
... performed TUNEL staining of tissue sections from ...mice, TUNEL staining of PDLN sections harvested from NOD mice revealed an increased frequency of apoptotic cells as early as 3 days after ...
12
Assessment of morphological and functional changes in neonate vitrified testis grafts after host treatment with melatonin
... Representative TUNEL-stained sections of vitrified-thawed testis graft from melatonin-treated and non-treated groups during the examined weeks; ...A. TUNEL staining of testis graft sections from the ...
8
Temperature-dependent and time-dependent effects of hyperthermia mediated by dextran-coated La<sub>0.7</sub>Sr<sub>0.3</sub>MnO<sub>3</sub>: in vitro studies
... placed in Petri plates. After 48 hours, the cells were exposed to RF-induced Dex-LSMO-mediated hyperthermia (43 ° C, 45 ° C, or 47 ° C) . At 0 hours and after 24 hours of treatment, the cells were fixed with ...
15
An experimental platform for systemic drug delivery to the retina
... modulation. TUNEL staining became apparent 12 h after the initial exposure to light; however, significantly less cell death was observed in the group of mice that received ALLM when the iBRB was modulated ...
6
Original Article Protective effects of LM22A-4 on injured spinal cord nerves
... a TUNEL assay and Nissl staining were performed to study apoptosis and the survival of ...(TUNEL staining and caspase-3 and Bcl-2 expression) compared with vehicle treated group (P < ...
7
Chandipura Virus Induces Neuronal Death through Fas-Mediated Extrinsic Apoptotic Pathway
... CHPV infection leads to neuronal cell death. Phase-contrast mi- croscopic images showed significant cell shrinkage and membrane blebbing in CHPV-infected cells (both in mouse neuroblastoma, i.e., Neuro2A, and human ...
9
Rapamycin Prevents cyclophosphamide-induced Over-activation of Primordial Follicle pool through PI3K/Akt/mTOR Signaling Pathway in vivo
... study, TUNEL staining showed an evidently suppressed apoptosis of granulosa cells in mature antral follicles in CY + Rapa group as compared to CY alone group, along with the absence of apoptotic death of ...
11
Anti-high mobility group box-1 (HMGB1) antibody attenuates kidney damage following experimental crush injury and the possible role of the tumor necrosis factor-α and c-Jun N-terminal kinase pathway
... In the second phase, 60 mice were randomly assigned to 5 groups: CS (n = 12), CS + vehicle (n = 12), CS + anti-HMGB1 antibody (n = 12), CS + anti-TNF-α anti- body (n = 12), and CS + SP600125 (n = 12). The CS model was ...
7
Xenotropic and polytropic retrovirus receptor 1 (XPR1) promotes progression of tongue squamous cell carcinoma (TSCC) via activation of NF-κB signaling
... IHC staining of XPR1 and Ki67, as well as TUNEL staining in xenograft ...of TUNEL-positive ...IHC staining in 128 TSCC patient ...
12
Intracellular pH-responsive and rituximab-conjugated mesoporous silica nanoparticles for targeted drug delivery to lymphoma B cells
... via TUNEL staining assay and reduced systemic toxicity of RDMSNs could probably be involved in the following factors: (1) DOX molecule loaded into the hole of MSNs effectively protected it from quick ...
14
Movement deficits and neuronal loss in basal ganglia in TRPC1 deficient mice
... To further determine whether the neuronal loss was related to apoptosis, we evaluated the effects of TRPC1 depletion on neuronal apoptosis using TUNEL staining. The results showed that TRPC1 depletion could ...
10
<p>SIRT1 inhibits hepatocellular carcinoma metastasis by promoting M1 macrophage polarization via NF-κB pathway</p>
... subsequent TUNEL assay. Cells with positive TUNEL staining were markedly increased in HepG2 cells by conditioned medium generated from SIRT1-transfected macrophages, when compared with that from ...
11
Neuroprotective and antioxidant effects of ghrelin in an experimental glaucoma model
... the TUNEL assay, nuclei stained blue with Harris hematoxylin were considered normal, and cells with brown nuclear staining were deemed ...of TUNEL staining was performed based on the extent of ...
11
Original Article Exendin-4 protects retinal pigment epithelial cells against oxidative stress in vitro via PI3K/Akt-STAT3 activation
... The TUNEL staining for cellular apoptosis was performed to evaluate the apoptosis extent according to the manufacturer’s instructions of TUNEL detection kit (Nanjing Jiancheng Bio- engineering ...
10
Original Article Combination of Salidroside and hyperbaric oxygen improves leukoaraiosis through P13K/Akt signaling
... protein) staining were used to observe the changes of white matter myelin and oligodendrocytes, TUNEL was used to observe the apoptosis of oligoden- drocytes and phosphor P13K/Akt signaling was assayed by ...
6
Correlation between the Hypoosmotic SwellingT and DNA Fragmentation Assessed by the TUNEL Assay in Asthenozoospermia
... the TUNEL assay are that it is a time-consuming technique, which requires specific skills and, most importantly, causes sperm toxicity. Therefore, the adoption of a nontoxic sperm selection test is essential for ...
6
Efferocytosis produces a prometastatic landscape during postpartum mammary gland involution
... Annexin V staining revealed that this approach induced cell death in more than 70% of all MCF7 cells (Supplemental Figure 11). After 16 hours of coculture, we used ELISAs to measure protein levels of mouse IL-4 ...
17