two-photon fluorescence imaging
Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... using two-photon excited fluorescence lifetime imaging ...the fluorescence lifetime of fluorophore and the refractive index of its microen- vironment varying due to changes in the ...
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Super resolution Imaging by Two photon Structured Illumination Microscopy
... dynamic imaging ability of living cells and the advantage of specificity mark make it widely used in the life science, materials, and polymer field ...conventional fluorescence microscopy is limited by the ...
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Interaction of Poxvirus Intracellular Mature Virion Proteins with the TPR Domain of Kinesin Light Chain in Live Infected Cells Revealed by Two-Photon-Induced Fluorescence Resonance Energy Transfer Fluorescence Lifetime Imaging Microscopy
... Construction of plasmids. A plasmid that encodes enhanced green fluorescent protein was constructed in two steps. Briefly, the DNA spanning the coding sequence of EGFP was amplified from pEGFP-C1 vector (Molecular ...
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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles
... be fluorescence from GFP, no strong fluorescence signal but only weak cell autofluorescence can be seen in Figure ...same imaging region is examined under two-photon ...the ...
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Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser
... the fluorescence are reported and discussed in literature, and commonly attributed to saturation of the dye or photo-bleaching, but also to stimulated emission, excited state absorption, residual one-photon ...
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Measurements of Absolute Atomic Nitrogen Density by Two Photon Absorption Laser Induced Fluorescence Spectroscopy in Hot Air Plasma Generated by Microwave Resonant Cavity
... OES is used to estimate gas temperature along the axial and radial directions of the air plasma column generated by the MW resonant cavity and launched inside the gas-conditioning cell. We used a spectrophotometer with ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... on fluorescence or Raman spectroscopy [4,5]. Fluorescence methods have intrinsically high sensitivity with the ability to report on molecular processes and environmental effects [6] and depend either on the ...
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Time-domain fluorescence lifetime imaging techniques suitable for solid-state imaging sensor arrays
... to two gated counters without actually carrying out the experiments probably because of the complexity and the cost of reconfiguring the existing acquisition ...and photon efficiency (the photon ...
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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre
... live imaging of biological tissue 1 . Two types of laser source suited to two-photon excitation exist, namely continuous wave (cw) and ultra-short pulsed (USP) lasers, the latter delivering ...
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Towards Heralded Two-Photon Absorption and Two-Photon Excited Fluorescence for Quantum Microscopy and Spectroscopy
... resolution imaging technologies used in the life sciences sector [9] exploiting ...of two- photon microscopy are the deeper penetration depth into the tissue and a drastically increased depth ...
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Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... trol (Figure 4, panels A1-3) and next on cells co-expressing Vpr-eGFP and mCherry fusion proteins (Figure 4, panels B1-3 and C1-3). An arbitrary color scale, ranging from blue to red, illustrates short to long lifetimes. ...
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
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Recent advances in hydrogen peroxide imaging for biological applications
... resolution imaging with lower phototoxicity and deeper tissue penetration than confocal microscopy ...the two-photon process, a molecule simultaneously absorbs two photons whose individual en- ...
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Near-field correlations in the two-photon field
... Figure 7 shows how the central peak for transverse corre- lations can get very narrow in certain configurations. We show the results obtained for a transverse scan at z= 0 and = −8.2. The full width at half maximum ...
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Droste genannt Helling, Imke (2009): Imaging cellular mechanisms of presynaptic structural plasticity. Dissertation, LMU München: Fakultät für Biologie
... least two different types have been investigated in detail: STVs (Synaptic vesicle proteins transport vesicles), which transport synaptic vesicle proteins, and PTVs (piccolo transport vesicles), which transport ...
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Two-photon imaging of the mammalian retina with ultrafast pulsing laser
... vivo imaging, ERG measurements, histology, immunohistochemistry, and the quantification of visual pigment and visual chromophore, no deleterious changes to the structure and function of the retina occurred after ...
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Three photon light sheet fluorescence microscopy
... transform along the axial direction (Fig. 3(b)). The MTF con- cisely represents information of both resolution and contrast. We set a practical noise-floor at 5% contrast to determine the maximum axial resolution, which ...
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Prototype Nerve-Specific Near-Infrared Fluorophores
... logD at pH 7.4 between 0.5 to 3 and molecular weight < 500 Da to maximize blood-nerve-barrier (BNB) penetration, 2) excitation and emission wavelengths in the NIR window, and 3) prolonged retention in the nerve tissue ...
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Enhanced frequency up-conversion in Rb vapor
... via two-photon resonances and in particular by using electromagnetically induced transparency ...lived two-photon coherences at frequencies that allow propagation under phase-matching ...
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Single and two photon time resolved polarised fluorescence studies of probe molecule dynamics in nematic liquid crystals
... single photon and two photon data indicate a preferential molecular orientation at 35 ° -45 ° to the nematic director, however both display regions of negative probability indicating that additional ...
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