two-photon laser scanning fluorescence microscopy
Two-photon laser scanning fluorescence microscopy using photonic crystal fibre
... USP laser gain medium for TPLSM is currently ...The laser source is typically used in conjunction with a computer-controlled scan-head that manipulates the beam profile of the entering laser, and a ...
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Towards Heralded Two-Photon Absorption and Two-Photon Excited Fluorescence for Quantum Microscopy and Spectroscopy
... a fluorescence photon is emitted subsequently to the absorption process (see ...derived two-photon absorption theoretically in her thesis in 1931 using quantum theory and the Dirac notation ...
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Methanol immersion reduces spherical aberration of water dipping lenses at long wavelengths used in multi-photon laser scanning microscopy
... multi-photon laser scanning microscopy, since their large working distances are advantageous for thick specimens and the absence of a coverslip facilitates examination of living ...in ...
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Dual-modality optical diagnosis for precise in vivo identification of tumors in neurosurgery
... on fluorescence and surface-enhanced Raman scattering for in vivo tumor ...(OCT-laser-induced fluorescence spectro- scopy) to a murine colon cancer model and successfully identified the cancerous ...
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Bioluminescence imaging and two-photon microscopy guided laser ablation of GBM decreases tumor burden
... the fluorescence intensity. Laser resection included the two ...Nd:YAG laser source with a wavelength of 1,064 nm (HGL-MY100C, Wuhan Huagong Laser Engineering, China) in the ...
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Three photon light sheet fluorescence microscopy
... pulsed laser beam was expanded to illu- minate a single-axis galvanometric mirror (Thorlabs) driven by a triangular wave ...the scanning mirror onto the back aperture of the illumination objective (Nikon, ...
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... a laser scanning confocal microscope (TSC SP5 & DMI 6000 B, Leica, Wetzlar, Germany) with a 63X oil-immersed objective ...Argon-ion laser operating at 514 nm was used in combination with the ...
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Recent advances in hydrogen peroxide imaging for biological applications
... confocal microscopy [28]. In the two-photon process, a molecule simultaneously absorbs two photons whose individual en- ergy is only half of the energy needed to excite that mol- ecule, and ...
10
Fluorescence excitation analysis by two-photon confocal laser scanning microscopy: a new method to identify fluorescent nanoparticles on histological tissue sections
... of fluorescence intensity of each pixel in the spectral emission image, or excitation (Figure ...of fluorescence patterns, and the aim is to unmix those ...a two-step ...
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Reduction of the pulse duration of the ultrafast laser pulses of the Two Photon Laser Scanning Microscopy (2PLSM)
... Glan Laser polarizer GL10. This polarizer consists of two prisms separated by air ...the two prisms uses to delay the red wavelength when the light leaves the first prism to the air then to second ...
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Measurements of Absolute Atomic Nitrogen Density by Two Photon Absorption Laser Induced Fluorescence Spectroscopy in Hot Air Plasma Generated by Microwave Resonant Cavity
... The fluorescence light, emitted by a small volume centered at the focused spot is collected perpendicularly to the laser beam and focused by a BK7 lens with a focal length of 75 mm onto the photocathode of ...
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Microscanners for optical endomicroscopic applications
... lens scanning has also been developed for for- ward looking endomicroscopy by using electromagnetic, electrothermal, or electrostatic ...a two- axis MEMS mirror whose size is ...optical scanning for ...
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Enhancing microparticle internalization by nonphagocytic cells through the use of noncovalently conjugated polyethyleneimine
... To calculate the efficiency of microparticle uptake, two different strategies were used. FC studies allowed us to clearly identify the population of cells associated with microparticles but also to discriminate ...
12
Near-field scanning optical microscopy with laser trapping
... and Ke P.C, "Effect of depolarisation of scattered evanescent waves on particle- frapped near-field scannuig optical microscopy", Appl. Gu M., Advanced Opticallmaging Theory (Spr[r] ...
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IMAGING OF FLUOROPHORES IN CHROMATOGRAPHIC BEADS, RECONSTRUCTION OF RADIAL DENSITY DISTRIBUTIONS AND CHARACTERISATION OF PROTEIN UPTAKING PROCESSES
... optical microscopy in order to observe mass transfer in spherical absorbent particles, but this technique seems not to be applicable for a quantitative description of diffusion processes in the ...
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Three dimensional modelling of building using faro laser scanner and autocad 2011 software
... Next, after all scans data were already filtered, it needs to be registered to produce a complete 3D point clouds model (Figure 8) of the building. For registration process, a minimum of 3 reference target or more must ...
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The Relationship of Surface Roughness and Work Function of Pure Silver by Numerical Modeling
... Surface of silver has important effects on its performance in natural environments. Surface roughness and work function of the silver samples were measured using confocal laser scanning microscopy ...
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Madhira, Sushi (2017): Single-molecule multi-color FRET: from DNA to protein. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Single-molecule fluorescence techniques are a powerful tool that enable monitoring of the conformational dynamics of biomolecules in real time, whether in solution or tethered to the ...reflection ...
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A surface plasmon enhanced FLIM-FRET imaging approach based on Au nanoparticles
... a two-photon fluorescence lifetime imaging microscopy (FLIM) method monitoring the Foster resonance energy transfer (FRET) effect between gold nanorods (GNRs) particles and fluorescent ...
5
Liebscher, Sabine (2017): Structural and functional alterations of cortical neurons in Alzheimer’s disease transgenic mice assessed by two-photon in vivo imaging. Dissertation, LMU München: Graduate School of Systemic Neurosciences (GSN)
... Having demonstrated the impact of amyloid plaques on synaptic structures, I next asked whether pharmacological inhibition of Aβ generation can have beneficial effects on the stability of synaptic structures. ...
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