viral double-stranded RNA
Effect of Viral Double-Stranded RNA on Protein Synthesis in Intact Cells
... The viral double-stranded RNA used in Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The addition of purified, noninfectious, double-stranded RNA of bovine enterovirus[r] ...
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Effect of Viral Double-Stranded RNA on Mammalian Cells in Culture: Cytotoxicity Under Conditions Preventing Viral Replication and Protein Synthesis
... Effect of Viral Double-Stranded RNA on Mammalian Cells in Culture: Cytotoxicity Under Conditions Preventing Viral Replication and Protein Synthesis Received for publication 8 January 197[r] ...
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Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA
... VP3 interacts both with VP1 and with IBDV-specific dsRNA. To study the VP1-VP3 interaction per se, i.e., without the interference of RNA-mediated coimmunoprecipitation, we performed an RNase treatment on all the ...
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Conservative replication and transcription of Saccharomyces cerevisiae viral double-stranded RNA in vitro.
... Our present experiments demonstrate for the first time that replication synthesis of minus strand occurs in virus particles in vitro and that ScV transcription and replication are conser[r] ...
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Structure of a VP1-VP3 Complex Suggests How Birnaviruses Package the VP1 Polymerase
... Infectious pancreatic necrosis virus (IPNV), a member of the family Birnaviridae, infects young salmon, with a severe impact on the commercial sea farming industry. Of the five mature proteins encoded by the IPNV genome, ...
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Polioviruses, Coxsackieviruses, and Echoviruses: Comparison of the Genomes by RNA Hybridization
... The methods for extraction and purification of viral double-stranded RNA dsRNA from infected cells by treatment with hot phenol, differential salt preparation of singlestranded RNA ssRNA[r] ...
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Overlapping genes in a yeast double-stranded RNA virus.
... One of the products of in vitro translation of the denatured viral double-stranded RNA is a polypeptide of the size expected of a capsid-polymerase fusion protein, resulting from a -1 fr[r] ...
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Stress Granules Regulate Double Stranded RNA Dependent Protein Kinase Activation through a Complex Containing G3BP1 and Caprin1
... S tress granules (SGs) are dynamic cytoplasmic foci containing translationally silenced messenger RNP (mRNP) condensates which form in response to a wide range of cellular stresses that inhibit protein synthesis through ...
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Identification of an RNA Silencing Suppressor from a Plant Double-Stranded RNA Virus
... host RNA silencing ...an RNA silencing suppressor ...however, RNA silencing suppressors have not been ...and viral proteins involved in viral particle assembly have been intensively ...
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Unique double-stranded RNAs associated with the Trichomonas vaginalis virus are synthesized by viral RNA-dependent RNA polymerase.
... On the basis of present information, TVV lacks an infectious cycle and virus-negative isolates are resistant to infection by purified virus particles obtained from the cytoplasmic fracti[r] ...
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Double-Stranded RNA Is Detected by Immunofluorescence Analysis in RNA and DNA Virus Infections, Including Those by Negative-Stranded RNA Viruses
... of viral replication suggested that most viruses produce dsRNAs ...in double-stranded DNA (dsDNA) and positive-strand RNA virus infections but not in negative- strand RNA virus ...
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LRV1 viral particles in Leishmania guyanensis contain double-stranded or single-stranded RNA.
... The relative positions of the particles containing dsRNA and ssRNA in a CsCl density gradient differ slightly depending on the method used for cell disruption data not shown.. These resu[r] ...
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Genome organization of RNA tumor viruses. I. In vitro synthesis of full-genome-length single-stranded and double-stranded viral DNA transcripts.
... Collett and Faras 6 observed that at a concentration of 200 ,um of each 615 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Genome-length complementary DNA cDNA transcr[r] ...
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Denaturation and Renaturation of Viral Ribonucleic Acid II. Characterization of the Products Resulting from Annealing R17 Ribonucleic Acid with Denatured Replicative Form or with Denatured Replicative Intermediate
... After digestion with ribonuclease, the double-stranded RNA found in the salt precipitate had a sedimentation profile identical to that of the doublestranded RNA found in the salt superna[r] ...
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Template Role of Double-Stranded RNA in Tombusvirus Replication
... plus-strand RNA [(ⴙ)RNA] viruses of plants is a relatively simple process that involves complementary minus- strand RNA [(ⴚ)RNA] synthesis and subsequent (ⴙ)RNA ...of viral ...
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Noncytopathic Bovine Viral Diarrhea Virus Inhibits Double-Stranded RNA-Induced Apoptosis and Interferon Synthesis
... Bovine viral diarrhea virus (BVDV), a pestivirus of the Flaviviridae family, is an economically important cattle pathogen with a worldwide ...synthetic double-stranded RNA ...of viral ...
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Double-Stranded RNA Binding by Human Cytomegalovirus pTRS1
... The dsRBD of pTRS1 is not sufficient for restoration of VV ⌬ E3L host cell range. The dsRBD of pE3L is necessary and sufficient for rescue of VV ⌬ E3L replication in nonpermissive cell lines such as HeLa cells (44). To ...
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Deletion of Cytoplasmic Double-Stranded RNA Sensors Does Not Uncover Viral Small Interfering RNA Production in Human Cells
... their viral suppressor of RNAi (VSR) (7, 8, ...of viral RNA by the RNAi machinery ...upon viral infection and stimulation with dsRNA mimics (18, ...
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Delayed Cytosolic Exposure of Japanese Encephalitis Virus Double-Stranded RNA Impedes Interferon Activation and Enhances Viral Dissemination in Porcine Cells
... The rapid response and efficiency of the host to establish and maintain an antiviral state puts viruses at a disadvantage. Nev- ertheless, viruses have developed various defense mechanisms to frustrate the host immune ...
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Transcription In Vitro by Reovirus-Associated Ribonucleic Acid-Dependent Polymerase
... To test whether all or a limited number of genome segments are transcribed in vitro, mixtures of single-stranded product and double-stranded viral RNA were analyzed by polyacrylamide gel[r] ...
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