Viral nonstructural proteins NS1
Noroviruses Co opt the Function of Host Proteins VAPA and VAPB for Replication via a Phenylalanine–Phenylalanine Acidic Tract Motif Mimic in Nonstructural Viral Protein NS1/2
... MNoV NS1/2 sequences to host FFAT motifs was identified in the N-terminal and C-terminal portions of the ...host proteins) tolerates a wide range of residue substitutions without loss of ...the NS1 ...
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Deletion of Nonstructural Proteins NS1 and NS2 from Pneumonia Virus of Mice Attenuates Viral Replication and Reduces Pulmonary Cytokine Expression and Disease
... qRT-PCR and ELISA. The expression of IFN- ␣ , IFN-  , CXCL10, IRF7 mRNA, and PVM genomic RNA in MEF cells was detected by quantitative RT-PCR (qRT-PCR). Triplicate cultures in six-well dishes were mock infected or ...
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Transfectant Influenza A Viruses with Long Deletions in the NS1 Protein Grow Efficiently in Vero Cells
... structural proteins and one nonstructural protein (NS1). The NS1 protein is abundant in influenza virus-infected cells but has not been detected in ...virions. NS1 is a phosphoprotein ...
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Development of Interest in Science and Interest in Teaching Elementary Science: Influence of Informal, School, and Inquiry Methods Course Experiences
... a viral nonstructural protein as well as with the 3’(-)SL ...NS5, NS1, or E, was co-immunoprecipatated by both anti-TIA-1 and anti-TIAR ...hydrophobic nonstructural proteins (NS2a, ...
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The Secreted Form of Dengue Virus Nonstructural Protein NS1 Is Endocytosed by Hepatocytes and Accumulates in Late Endosomes: Implications for Viral Infectivity
... cellular proteins such as Alix, drives the formation of intralumenal membranous structures constitutive of late endosomes (26, 43), thereby representing a specific marker of the late endosomal compartment in ...
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H-1 Parvovirus-Associated Replication Bodies: a Distinct Virus-Induced Nuclear Structure
... major nonstructural protein (NS1) of H-1 virus which is essential for viral DNA amplification colocalized with virus-specific DNA sequences and sites of ongoing viral DNA replication in ...
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Caspase Cleavage of the Nonstructural Protein NS1 Mediates Replication of Aleutian Mink Disease Parvovirus
... against viral in- fection is ...encode proteins that inhibit apoptosis induction in an effort to prolong cell survival and aid virus replication (15, 19, 20, ...
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Genetic Interaction of Flavivirus Nonstructural Proteins NS1 and NS4A as a Determinant of Replicase Function
... their NS1 proteins for the same function(s), it seems unlikely that a protein required for RNA replication would demonstrate high functional diver- gence among closely related ...YF NS1, we ...
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The nonstructural proteins of Pneumoviruses are remarkably distinct in substrate diversity and specificity
... V proteins of the Rubulavirus genus of this family, all of which degrade STAT proteins by assem- bling cellular proteasomes and possess conserved sequences such as a Cys-rich zinc-binding domain ...NS ...
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Dengue virus non-structural protein 1: a pathogenic factor, therapeutic target, and vaccine candidate
... structural proteins: capsid (C, 100 amino acids), precursor membrane (prM, 75 amino acids), and envelope (E, 495 amino acids) ...the viral RNA genome form a nucleocapsid that buds at the endoplasmic ...
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Dense Bodies of Human Cytomegalovirus Induce both Humoral and Cellular Immune Responses in the Absence of Viral Gene Expression
... DB have been well recognized by HCMV researchers pri- marily as an obstacle to the purification of virions. These non- infectious particles are released in large amounts after infec- tion of primary human fibroblasts ...
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Picornavirus nonstructural proteins: emerging roles in virus replication and inhibition of host cell functions.
... In contrast, the identities of the host cell proteins which cooperate with the viral nonstructural proteins in virus replication e.g., ribosomeassociated P36, host factor, 3CD-stimulatin[r] ...
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C-Terminal nsP1a Protein of Human Astrovirus Colocalizes with the Endoplasmic Reticulum and Viral RNA
... Double labeling of HAstV proteins and viral RNA. nsP1a/4 detection was performed by immunofluorescence, and viral RNA was detected by fluorescence in situ hybridization with a digoxigenin-labeled DNA ...
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Translational Inhibition and Increased Interferon Induction in Cells Infected with C Protein-Deficient Measles Virus
... other proteins, the V and C proteins, by an RNA editing process and by alternative initiation of translation in a different reading frame, respectively ...inhibit viral transcription and genome ...
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Rhesus Rotavirus Entry into a Polarized Epithelium Is Endocytosis Dependent and Involves Sequential VP4 Conformational Changes
... tralizing antibodies (Abs) that, in part, block RV entry (52). In order to efficiently mediate productive infection, RV penetra- tion of the epithelial cell membrane requires an initial trypsin- mediated proteolytic ...
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Structural and Nonstructural Proteins of Saint Louis Encephalitis Virus
... Identification of the proteins synthesized in the infected cells as being viral structural or nonstructural was accomplished by co-electrophoresis of cytoplasmic extracts from 3H-amino a[r] ...
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Immunization of mice with dengue structural proteins and nonstructural protein NS1 expressed by baculovirus recombinant induces resistance to dengue virus encephalitis.
... 3027-3031 0022-538X/88/083027-05$02.00/0 Copyright X 1988, American Society for Microbiology NOTES Immunization of Mice with Dengue Structural Proteins and Nonstructural Protein NS1 Expr[r] ...
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Rotavirus protein rearrangements in purified membrane-enveloped intermediate particles.
... To characterize further the positions of the viral structural and nonstructural proteins in the intermediate immunoisolated particle, we treated material specifically bound to the Dynal [r] ...
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Detection of foot and mouth disease virus infected cattle by assessment of antibody response in oropharyngeal fluids
... coat proteins of bovine enteroviruses ...tural viral proteins such as 3D is actually cumbersome; in fact, multivaccinated cattle can be 3D antibody positive (15) and concentrated, inactivated FMDV ...
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Evidence that flavivirus NS1-NS2A cleavage is mediated by a membrane-bound host protease in the endoplasmic reticulum.
... DEN4 NS1-NS2A cleavage apparently uses an 8-aa cleav- age recognition site (positions 2 8 to 2 1, MVKSQVTA) and site-directed mutagenesis experiments have demonstrated that the amino acids at 2 8, 2 7, 2 5, 2 3, ...
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