viral vectors
From Agrobacterium to viral vectors: genome modification of plant cells by rare cutting restriction enzymes
... a viral-based expression system for efficient delivery of rare-cutting restriction enzymes to plant ...Several vectors were designed to facilitate the transport of single- and dual-monomer restriction ...
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Transduction of Dendritic Cells by DNA Viral Vectors Directs the Immune Response to Transgene Products in Muscle Fibers
... to viral infection in animal ...DNA viral vectors has important implications in the develop- ment of safe and effective adenovirus ...with vectors that contain more specific promoters, which ...
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Influenza viral vectors expressing the Brucella OMP16 or L7/L12 proteins as vaccines against B abortus infection
... Flu-NS1-124-Omp16-H5N1, Flu-NS1-124-L7/L12-H1N1 and Flu-NS1-124-Omp16-H1N1. Despite of substantial modification of NS1 gene, all constructs replicated well and were retain their Brucella inserts over five passages in ...
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Breaking the Bonds: Non-viral Vectors Become Chemically Dynamic
... categories, viral and non-viral. Viral vectors are derived from viruses by the use of recombinant DNA techniques and are most favored because they can efficiently deliver genes into a variety ...
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Tailoring a Plasmodium vivax Vaccine To Enhance Efficacy through a Combination of a CSP Virus-Like Particle and TRAP Viral Vectors
... and viral vectors requires vivax antigens in both platforms to enhance protective efficacy against a sporozoite ...with viral vectors expressing an unrelated Trypanosoma cruzi antigen, Tc24 ...
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Differential Effects of Viral Vectors on Migratory Afferent Lymph Dendritic Cells In Vitro Predict Enhanced Immunogenicity In Vivo
... vaccine viral vectors: recombinant human replication-defective human adenovirus 5 (rhuAdV5), recombinant modified vaccinia virus Ankara (rMVA), and recombinant fowlpox virus (rFPV), all expressing green ...
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Viral vectors as a novel tool for clinical and neuropsychiatric research applications
... nologies are new technologies for genomic modification. The principles of these techniques are to induce genomic double-stranded DNA breaks at specific loci. The target gene is then engineered by non-homologous end ...
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Characterization of Promoter Function and Cell-Type-Specific Expression from Viral Vectors in the Nervous System
... Adenoviral vectors have been used extensively for gene transfer into ...adenovirus vectors to drive expres- sion of foreign genes in neurons (6, 7, 12, 28, 29, ...the viral vector remains largely ...
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The production of viral vectors designed to express large and difficult to express transgenes within neurons
... using viral vectors that could deliver these transgenes to neurons within the ...a viral vector that can be produced at high titers and capable of conferring the ex- pression of these transgenes ...
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THE USE OF VIRAL VECTORS IN GENE TRANSFER THERAPY
... between simple retroviruses (such as Moloney mice leukemic virus) and lentiviruses is, that simple retroviruses hold only three structural genes, while lentiviruses contain nine (three structural – gag, pol, env, and six ...
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Immunogenicity of Cytopathic and Noncytopathic Viral Vectors
... A major criticism of previous studies with similar objectives (13, 21, 29) is the use of model cytopathic and noncytopathic viruses that are markedly different with respect to natural host, cell tropism, virulence, and ...
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Engineered Viral Vectors and Developed Tissue Clearing Methods for Single cell Phenotyping in Whole Organs
... encoding vectors that exist in the off-state until induced by a second AAV vector that can be delivered at an empirically determined dose to provide expression in the desired fraction of co-transduced ...
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Capsid antigen presentation flags human hepatocytes for destruction after transduction by adeno associated viral vectors
... Adeno-associated virus (AAV) vectors are effective gene delivery vehicles mediating long-lasting transgene expression. Data from a clinical trial of AAV2-mediated hepatic transfer of the Factor IX gene (F9) into ...
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Novel viral vectors utilizing intron splice switching to activate genome rescue, expression and replication in targeted cells
... of these SSOs or their combination resulted in up to a two-fold activation of rescue and/or subsequent replica- tion of the B1 vector containing only wt introns. A simi- lar enhancement effect on SFV infection was often ...
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Sex significantly influences transduction of murine liver by recombinant adeno-associated viral vectors through an androgen-dependent pathway.
... AAV vectors will be moderated by an androgen-dependent ...AAV vectors that target the liver as females and juvenile male patients are likely to have a substantially lower transduction efficiency than ...
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Factors influencing in vivo transduction by recombinant adeno-associated viral vectors expressing the human factor IX cDNA.
... Although these pioneering studies have clearly established the potential of rAAV-mediated gene therapy for hemophilia B, a number of issues remain unresolved. Various vectors and routes of administration have been ...
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Serotype dependent packaging of large genes in adeno associated viral vectors results in effective gene delivery in mice
... Vectors derived from adeno-associated virus (AAV) are promising for human gene therapy, including treat- ment for retinal blindness. One major limitation of AAVs as vectors is that AAV cargo capacity has ...
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Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication deficient viral vectors
... these viral con- structs undergo only one single round of replication resulting in the production of non-infectious virus-like particles in ...The vectors were either given systemi- cally or exclusively ...
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The emerging role of viral vectors as vehicles for DMD gene editing
... Duchenne muscular dystrophy (DMD) is a genetic disorder caused by mutations in the dystrophin-encoding DMD gene. The DMD gene, spanning over 2.4 megabases along the short arm of the X chromosome (Xp21.2), is the largest ...
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Recombinant Adeno-Associated Viral Vectors Are Deficient in Provoking a DNA Damage Response
... limit viral replication and ...rAAV2 vectors may attract the MRN complex, but the induced DNA damage response in that case is not as strong as the one seen when stalled DNA replication provokes a DNA damage ...
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