Ziehl-Neelsen staining.
Prospective study to find out the role of gastric aspirate examination by Ziehl-Neelsen staining (ZN staining) and cartridge based nucleic acid amplification test (CB-NAAT) as a diagnostic method in childhood tuberculosis
... All the selected patients were subjected to detailed history, general physical examination, systemic examination, haematological investigations, tuberculin sensitivity test and gastric aspiration for ...
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A comparative study on ziehl neelsen staining and immuno histochemical analysis in suspected tuberculous lesions
... Ziehl-Neelsen staining which is a fast and cheap procedure, has a variable sensitivity which depends on the number of bacilli in the ...of Ziehl-Neelsen staining, is soluble in ...
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COMPARISION OF CONVENTIONAL DIANOSTIC MODALITIES (FINE NEEDLE ASPIRATION CYTOLOGY & ZIEHL-NEELSEN STAINING) WITH CULTURE (BACTEC) FOR THE DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS: A PROSPECTIVE STUDY
... Biopsy, Ziehl Neelson(ZN) staining, culture by Loweinstein –Jensen(LJ) ...Z-N staining for the diagnosis of TB Lymphadenopathy over culture (BACTEC) ...
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COMPARISON BETWEEN MODIFIED ZIEHL NEELSEN AND SAFRANIN METHYLENE BLUE STAINING TECHNIQUES FOR DIAGNOSIS OF CRYPTOSPORIDIUM INFECTION AMONG DIARRHEIC PATIENTS IN RABAK CITY, WHITE NILE STATE, SUDAN
... Modified Ziehl- Neelsen, Safranin methylene blue and Trichrome methylene blue were used to stain oocysts which were collected by concentration ...by Ziehl Neelsen stain and safranin- methylene ...
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Comparison of the two Diagnostic Methods Used for the Detection of Cryptosporidium Infection among HIV Patients in Osogbo, Nigeria
... cold Ziehl-Neelsen staining technique as describe by Cheesbrough [11] The slide was then air-dried and observed under the compound light microscope using 40x objective lens for the presence of ...
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Original Article Rapid and accurate identification of mycobacterium tuberculosis complex by simultaneous detection of 16S rRNA and IS6110 sequence in FFPE samples
... culture, Ziehl-Neelsen staining, PCR test for target gene, as well as high cost com- mercially available assays, such as AccuProbe (Gen-Probe ...well. Ziehl- Neelsen staining is ...
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Sensitivity of direct versus concentrated sputum smear microscopy in HIV infected patients suspected of having pulmonary tuberculosis
... to Ziehl- Neelsen staining did not significantly increase sensitivity for TB over direct smear microscopy differs from the results of five prior studies that found higher sensitivity in HIV-endemic ...
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Molecular Identification of Mycobacterium bovis from Post-mortem Inspected Cattle at the Abattoir and Slaughter Houses in Bauchi State, Nigeria
... This work was carried out in collaboration between all authors. Author ASS designed the study, did the sampling, run the Ziehl-Neelsen staining technique and wrote the first draft of the manuscript. ...
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The development of a rapid diagnostic system for difficult to culture human pathogens
... after staining they resist decolourization with acidified alcohol and also with strong mineral ...common staining procedure used is Ziehl-Neelsen staining (Pfyffer, Palicova 2011) which ...
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A comparative study of AFB smear and GeneXpert MTB/RIF assay in pulmonary and extrapulmonary specimens and detection of rifampicin resistance in a tertiary care hospital
... The smear preparation was done in biosafety cabinet level 2B. For sputum specimens, a direct smear and a concentration method smear (by Petroff’s method) were prepared and heat fixed. Acid fast bacilli (AFB) ...
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Comparison of Modified Fluorescent Method and Conventional Ziehl Neelsen Method in The Detection of Acid Fast Bacilli in Lymph Node Aspirates.
... The Ziehl-Neelson and the auramine-rhodamine staining were positive in ...auramine staining is more than that of ziehl-neelsen ...
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A PCR Colorimetric Microwell Plate Hybridization Assay for Detection of Mycobacterium tuberculosis and M avium from Culture Samples and Ziehl Neelsen Positive Smears
... Strains and clinical specimens. The mycobacterial strains (11 American Type Culture Collection [ATCC] strains and 44 clinical isolates) and the nonmyco- bacterial strains (4 ATCC strains and 9 clinical isolates) listed ...
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Use of DNA Extracts from Ziehl Neelsen Stained Slides for Molecular Detection of Rifampin Resistance and Spoligotyping of Mycobacterium tuberculosis
... of Ziehl-Neelsen staining of sputum ...from Ziehl-Neelsen- stained slides by identification of mutations in the rpoB gene using reverse line blot hybridization and DNA ...from ...
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Evaluation of PCR in Detection of Mycobacterium tuberculosis from Formalin Fixed, Paraffin Embedded Tissues: Comparison of Four Amplification Assays
... positive Ziehl-Neelsen staining result, and a negative culture ...11 Ziehl-Neelsen-positive, culture-neg- ative autopsy samples, PCR allowed us to obtain a positive amplification for ...
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Improved sensitivity of direct microscopy for Mycobacterium tuberculosis in lymph nodes: comparison of fluorescent microscopy and modified bleach method ZN microscopy along with conventional ZN staining method
... 16. Patel MM, Patel K, Italiya SL, Kaptan KR. Improved diagnosis of tuberculosis in lymph node cytology by bleach method for detection of acid fast bacilli in comparison to conventional Ziehl Neelsen ...
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FISHing Mycobacterium tuberculosis Complex by Use of a rpoB DNA Probe Bait
... 37°C. Ziehl-Neelsen staining was done using a com- mercially available cold Ziehl-Neelsen staining (Quick-TB kit; RAL Diagnostics, Martillac, ...
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Original Research Article A COMPARATIVE STUDY ON THE ACCURACY OF LIGHT EMITTING DIODE FLUORESCENT MICROSCOPY AND CONVENTIONAL SPUTUM SMEAR MICROSCOPY IN THE DIAGNOSIS OF PATIENTS WITH PULMONARY TUBERCULOSIS K. Kishore Kumar ¹, K. Sai Samrat * 2, S. Sony Reddy2
... AURAMINE STAINING USING LED FLUORESCENT MICROSCOPY WITH ZIEHL-NEELSEN STAINING IN THE DIAGNOSIS OF PULMONARY TUBERCU- LOSIS done by Saroj Golia et al 634 sputum samples collected from ...
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Isolation of Mycobacterium Tuberculosis in Body Fluids and Comparison of Conventional Methods with Polymerase Chain Reaction in a Tertiary Care Hospital Chennai.
... Samples were subjected to standard methods of diagnosis by smear study fluorescence & Ziehl –Neelsen staining, culture on solid medium Lowenstein-Jensen & Liquid medium Kirchner’s and mo[r] ...
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A CORRELATION STUDY OF FINE-NEEDLE ASPIRATION TECHNIQUE WITH ZIEHL–NEELSEN STAINS IN DIAGNOSIS OF TUBERCULOUS LYMPHADENITIS IN SOUTH-EAST RAJASTHAN, INDIA
... FNAC is an optimally selected, efficient, easy to perform, and economical test for initial diagnostic work upon patients with TB lymphadenitis, So patients can be put to treatment immediately without much delay, thus ...
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THE PRESENCE OF MYCOBACTERIUM SPECIES IN RAW MILK OBTAINED FROM LACTATING COWS IN LEWA AND DUDA OF VIMTIM WARDS, MUBI NORTH
... The percentage of positive cases of sampled dairy cow milk to CRT across farms was 2% out of 100 sampled animals. Farm ‘G’ at Lewa and farm ‘E’ at Duda had 1 positive case each [1/13(7.69%), 1/17(5.88%)] respectively. ...
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