[PDF] Top 20 Accumulation and Intranuclear Distribution of Unintegrated Human Immunodeficiency Virus Type 1 DNA
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Accumulation and Intranuclear Distribution of Unintegrated Human Immunodeficiency Virus Type 1 DNA
... situ unintegrated from integrated viral DNA by performing fluorescent in situ hybridization on cells in which stress-induced chromosome condensation had been induced, a procedure that contracts chromosomes ... See full document
9
Circular forms of unintegrated human immunodeficiency virus type 1 DNA and high levels of viral protein expression: association with dementia and multinucleated giant cells in the brains of patients with AIDS.
... HIV-1 DNA were used for a seminested PCR with the SLP-1 and gag 912 primer pair (first round) and the SLP-1 and L577 primer pair (second ...For unintegrated 1-LTR forms of ... See full document
6
cis-acting elements in human immunodeficiency virus type 1 RNAs direct viral transcripts to distinct intranuclear locations.
... RNA distribution, similar to that described for a minority of polyadenylated cellular RNA species ...the distribution of un- spliced HIV RNA in cultures of H9 cells chronically infected with HIV IIIB ... See full document
23
Wild-Type Levels of Nuclear Localization and Human Immunodeficiency Virus Type 1 Replication in the Absence of the Central DNA Flap
... chromosomal DNA (reviewed in references 14 and ...proviruses, unintegrated linears are converted into 1- and 2-LTR-containing DNA circles in the nuclei of infected cells (14, 21, ... See full document
9
Unusual Distribution of Mutations Associated with Serial Bottleneck Passages of Human Immunodeficiency Virus Type 1
... sequencing. DNA was extracted using an Instagene purification matrix (Bio-Rad) according to the man- ufacturer’s ...individual virus clones, a collection of overlapping sets of oligonucleotide primers was ... See full document
7
Blockade of Human Immunodeficiency Virus Type 1 Expression by Caveolin-1
... a type of plasma membrane raft. We observed that coexpression of human Cav-1 with human immunodeficiency virus type 1 (HIV-1) blocked virion produc- tion ... See full document
13
Persistent human immunodeficiency virus type 1 infection of monoblastoid cells leads to accumulation of self-integrated viral DNA and to production of defective virions.
... Our continuing efforts to characterize this model system reveal a temporal decrease in infectivity of cell-free virus preparations from persistently infected U937 cells the uncloned, per[r] ... See full document
8
Accumulation of human immunodeficiency virus type 1 DNA in T cells: results of multiple infection events.
... In contrast to most type C virus infections, cultures of human immunodeficiency virus type 1 HIV-1-infected T cells tend to undergo the persistent synthesis of viral DNA, with moderate t[r] ... See full document
6
The DNA Deaminase Activity of Human APOBEC3G Is Required for Ty1, MusD, and Human Immunodeficiency Virus Type 1 Restriction
... Since DNA cytosine deamination is critical for A3G-medi- ated restriction of Ty1, MusD, and HIV-1( ⌬ Vif), the apparent lack of involvement of cellular UDGs was ...HIV- 1( ⌬ Vif), we showed that UNG2 ... See full document
9
DNA Damage Sensors ATM, ATR, DNA-PKcs, and PARP-1 Are Dispensable for Human Immunodeficiency Virus Type 1 Integration
... that DNA-PKcs, if anything, may negatively interfere with HIV-1 integration, reminiscent of its recently proposed inhi- bition of adeno-associated virus integration ...using DNA-PKcs-deficient ... See full document
6
Rev Inhibition Strongly Affects Intracellular Distribution of Human Immunodeficiency Virus Type 1 RNAs
... One notable difference was, however, an association of the env intron signal with the periphery of the IGs in rev-minus cells. This suggests that partially spliced or unspliced RNA could, in the absence of Rev protein, ... See full document
12
Human immunodeficiency virus type 1 Vpu and cellular TASK proteins suppress transcription of unintegrated HIV 1 DNA
... We also demonstrated that homologous TASK proteins profoundly suppressed HIV-1 transcription. TASK-1 and TASK-3 proteins are constitutively expressed in primary CD4 + T cells and contribute 40% of the K + ... See full document
12
Human Immunodeficiency Virus Type 1 (HIV-1) Vpr Enhances Expression from Unintegrated HIV-1 DNA
... from unintegrated DNA (18, 55). Yet it is unclear how the unintegrated DNA template would have been capable of expressing ...from unintegrated DNA would require an ... See full document
11
Dissociation of unintegrated viral DNA accumulation from single-cell lysis induced by human immunodeficiency virus type 1.
... The cytopathic effects of human immunodeficiency virus type 1 HIV-1 infection are specific for cells that express the CD4 viral receptor and consist of syncytium formation and single-cel[r] ... See full document
11
Evidence for Gene Expression by Unintegrated Human Immunodeficiency Virus Type 1 DNA Species
... HIV-1 DNA with two tan- dem repeats of the LTR are templates for viral ...R7 virus, but now these cells were cultured in the presence of aphidicolin, a reversible inhibitor of cell DNA ... See full document
9
Vpr Is Required for Efficient Nef Expression from Unintegrated Human Immunodeficiency Virus Type 1 DNA
... Tat is expressed from HIV IN ⴚ , and the levels are not affected by Vpr. Here and in previous studies, the expression of all viral transcripts was demonstrated from integrase-defective viruses in infected cells, ... See full document
9
Simian Immunodeficiency Virus Integration Preference Is Similar to That of Human Immunodeficiency Virus Type 1
... Viral components of preintegration complexes (1, 4, 8, 22), including integrase, nucleocapsid protein (4, 28), and the at- tachment sites of the reverse-transcribed genome, contribute to the ability of ... See full document
6
Pseudodiploid Genome Organization Aids Full-Length Human Immunodeficiency Virus Type 1 DNA Synthesis
... HIV DNA synthesis is about 4 h under optimal intra- cellular conditions (24), and reverse transcription in the pres- ence of HU takes even longer due to limiting substrate availability (15, 23, ... See full document
9
A Human T-Cell Leukemia Virus Type 1 Regulatory Element Enhances the Immunogenicity of Human Immunodeficiency Virus Type 1 DNA Vaccines in Mice and Nonhuman Primates
... of DNA vaccines in clin- ical trials to date has led to the development of various strat- egies to improve the immunogenicity of these ...plasmid DNA vaccine. We demonstrated that the addition of the ... See full document
7
Organization of Immature Human Immunodeficiency Virus Type 1
... Wild-type HIV-1 Gag particles visualized by cEM. We have previously proposed a model for the arrangement of Gag polyproteins in immature HIV-1 particles (13). Here we pro- vide experimental evidence ... See full document
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