[PDF] Top 20 Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.
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Analysis of herpes simplex virus nucleoprotein complexes extracted from infected cells.
... Extracts were prepared as described previously 21 by thawing infected cell samples and mixing them with 9 volumes of lysing solution containing 10 mM Tris-hydrochloride pH 7.9-0.25% Down[r] ... See full document
13
Quantification and Analysis of Thymidine Kinase Expression from Acyclovir-Resistant G-String Insertion and Deletion Mutants in Herpes Simplex Virus-Infected Cells
... pulse-labeling infected cells with [ 35 S]methionine for 30 min at 5 hpi, followed by a chase in the presence of unlabeled methionine for 6 ...for analysis. During the pulse phase, the N-terminal ... See full document
9
Analysis of HCF, the Cellular Cofactor of VP16, in Herpes Simplex Virus-Infected Cells
... reduced virus expression in the tsBN67 cells at ...MOI, virus protein synthesis was only marginally higher in the rescued cells, and the ratio of virus yield at ...different from ... See full document
11
Characterization of VP22 in Herpes Simplex Virus-Infected Cells
... such complexes, it is also as likely that the native VP22 peak 1 form is not associated with VP16 and thus is an independent oli- gomer or an assembly with components other than ...particularly from the ... See full document
14
Nuclear Pore Composition and Gating in Herpes Simplex Virus-Infected Cells
... sedimentation analysis, two modifica- tions merit further ...of infected cells has not been examined, but it is possible that pore alterations that did not affect gating could affect ... See full document
8
Herpes Simplex Virus 1 ICP4 Forms Complexes with TFIID and Mediator in Virus-Infected Cells
... the cells were harvested and ICP4 protein complexes were isolated using the TAP pro- cedure as described in Materials and ...protein complexes were separated via SDS-PAGE and visual- ized via silver ... See full document
12
Cbl E3 Ligase Mediates the Removal of Nectin-1 from the Surface of Herpes Simplex Virus 1-Infected Cells
... uninfected cells that involves the removal of the HSV entry receptor Nectin-1 from the surface of the cells which the virus enters and in which it ...EGFR from the surface of the ... See full document
14
Immature Monocyte-Derived Dendritic Cells Are Productively Infected with Herpes Simplex Virus Type 1
... were infected with HSV-1 at ...ranging from 12 to 48 h p.i. Controls included mock-infected cells and isotype-matched antibody ...of cells were infected, indicating that a ... See full document
7
The Product of the Herpes Simplex Virus 2 UL16 Gene Is Critical for the Egress of Capsids from the Nuclei of Infected Cells
... transit from the nucleoplasm to the cytoplasm, C capsids must be recruited to the inner nuclear membrane (INM), where they undergo primary envelopment facilitated by the nuclear egress complex comprised of UL31 ... See full document
15
Isolation of Herpes Simplex Virus Procapsids from Cells Infected with a Protease-Deficient Mutant Virus
... Herpes simplex virus type 1 (HSV-1) capsid proteins assemble in vitro into spherical procapsids that differ markedly in structure and stability from mature polyhedral capsids but can be ... See full document
11
Human Corneal Cells and Other Fibroblasts Can Stimulate the Appearance of Herpes Simplex Virus from Quiescently Infected PC12 Cells
... of herpes simplex virus type 1 (HSV-1) from an in vitro model of long-term (quiescent) infection is ...(PC12) cells differentiated with nerve growth factor were infected with ... See full document
10
Suppression of apoptotic DNA fragmentation in herpes simplex virus type 1-infected cells.
... attached cells were resuspended with ...medium. Cells were pelleted at 2,000 3 g for 20 s and resuspended in 100 ml of phosphate-buffered ...was extracted by the Hirt method (13), with minor ...DNA ... See full document
5
Gene expression of herpes simplex virus. I. Analysis of cytoplasmic RNAs in infected xeroderma pigmentosum cells.
... MILLETTE* Department of Immunology and Microbiology, Wayne State University School of Medicine, Detroit, Michigan 48201 RNAs which are synthesized and accumulate in the cytoplasm of unin[r] ... See full document
8
RNA Synthesis in Cells Infected with Herpes Simplex Virus VII. Control of Transcription and of Transcript Abundancies of Unique and Common Sequences of Herpes Simplex Virus 1 and 2
... Control of Transcription and of Transcript Abundancies of Unique and Common Sequences of Herpes Simplex Virus 1 and 2 Received for publication 17 January 1973 Analysis of the kinetics of[r] ... See full document
7
Exploring the Nuclear Envelope of Herpes Simplex Virus 1-Infected Cells by High-Resolution Microscopy
... during herpes simplex virus 1 (HSV-1) infection in HeLa and Vero cells by employing preparation techniques at ambient and low temperatures for high-resolution scanning and transmission ... See full document
12
Nucleoprotein Complexes in Simian Virus 40-Infected Cells
... After labeling, the Downloaded from http://jvi.asm.org/ on November 11, 2019 by guest When African green monkey kidney cells BSC-1 were infected with simian virus 40 SV40 and extracted w[r] ... See full document
9
ND10 Components Relocate to Sites Associated with Herpes Simplex Virus Type 1 Nucleoprotein Complexes during Virus Infection
... HFFF-2 cells were grown on 13-mm- diameter glass coverslips and then infected with wt HSV-1 at a multiplicity of infection of 10 PFU per ...the cells were washed twice with phosphate-buffered saline ... See full document
12
Effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus.
... From these results, we conclude that ara-C has little effect upon early HSV-1 protein synthesis but causes a rapid drop of amino acid incorporation into late HSV-1 proteins which begins [r] ... See full document
10
Lifetimes of mRNA molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells.
... In addition, when the viral proteins made after the addition of actinomycin were further analyzed by gel electrophoresis, it was found that messengers for individual viral proteins trans[r] ... See full document
9
Redistribution of microtubules and Golgi apparatus in herpes simplex virus-infected cells and their role in viral exocytosis.
... for cells undergoing mitosis (13, 14) and for cells exposed to agents like nocodazole and colchicine, which bind tubulin heterodimers and inhibit their polymerization and thus induce progressive ... See full document
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