[PDF] Top 20 APOBEC3G Complexes Decrease Human Immunodeficiency Virus Type 1 Production

... A3G complexes decrease HIV-1 pseudovirus production and intracellular HIV-1 Gag ...antigen production 24 h after HeLa cells were cotransfected with ⌬ 8.9 HIV-1 and the ... See full document

... prepared 1 h after acute infection. To compare HIV and MoMLV, 1-h fractions containing the peak of MoMLV or HIV DNA were mixed 1:1, subjected to nuclease digestion, ...collected 1, 4, ... See full document

... incorporation into HIV-1 (8). The cellular expression and viral incorporation of these truncated species were also reported in that paper, except for hA3G104-246, which is also incorpo- rated efficiently into ... See full document

... Lanes: 1, PICs incubated without target; 2, PICs incubated with a circular target; 3 and 4, same reaction products as in lanes 1 and 2 but cleaved with SalI; 5 and 6, PICs treated with SalI and then ... See full document

... each virus was released from transfected cells at the WT level, we focused our attention on the early steps of HIV-1 ...HIV- 1 NL4-3 , class I mutant HIV-1 D64N/D116N (40), and class II ... See full document

... Western blot analysis. Protein samples were separated by SDS-15% PAGE, followed by blotting onto nitrocellulose membranes (Gelman Science). Western blots were probed with either rabbit anti-APOBEC3G ... See full document

... since virion-associated Vpr mutant R80A, which has lost its cell cycle-modulating activity, was unable to activate CAT ex- pression from the LTR. This early effect of Vpr on LTR- directed gene expression was shown to ... See full document

... codon-optimized HIV-1 Vif expression vector. The initial panel of mutants targeted Vif residues 2 to 60. Because some of the single-residue mutants were already constructed (for example, W11A and I18A), the double ... See full document

... The K22, K26, and Y30 residues in HIV-1 Vif influence the Vif-A3G interaction. Mutation of K22, K26, or Y30 abolished the HIV-1 Vif activity against A3G. However, these Vif mutants maintained their ... See full document

... Next, we explored protein-protein binding using a series of deletion mutants. We found that A3G deletion mutants en- compassing amino acids (aa) 55 to 384, 109 to 384, or 1 to 162, but not 163 to 384, could be ... See full document

... Human APOBEC3G and several other APOBEC3 proteins have been shown to inhibit the replication of a variety of retrotransposons and ...lentivirus, human immunodeficiency virus type ... See full document

... I domain and Gag protein detergent-resistant complex for- mation. The ability of Gag to form intracellular Triton X-100- resistant complexes has recently been identified in HIV-in- fected cells and is proposed to ... See full document

... folding defects, as shown by our protein chaperone coexpres- sion studies. Our reconstitution studies do not exclude the possibility that other proteins assist IN with integration in vivo. With LTR donor substrates ... See full document

... HIV-1 virus lysates (10, 22, 23), recombinant HIV-1 IN (Table 1; ...Lanes 1 and 2 were mutant DNA without IN, while lanes 3 and 4 were with ...the 1-kbp DNA ladder marker (not ... See full document

... expressed APOBEC3G that presumably has not yet completed its transition from an immature to a mature con- figuration, a process that presumably involves assembly of APOBEC3G into high-molecular-mass ... See full document

... membrane. Virus particles were concentrated by centrifugation through a 30% sucrose cushion at 100,000 ⫻ g for 2 h in a Sorvall Ultra80 ...tagged APOBEC3G in virion samples was performed with the ECL Plus ... See full document

... imal HIV-1 evolution and characteristic APOBEC3G-induced hypermutation in one LTNP (37). In another study of three LTNPs, the frequency of defective vif clones amplified from PBMC was similar to that found ... See full document

... separated by SDS-PAGE. Analysis of the gel revealed that both GST-Jnk and GST-p38 phosphorylated LSF in vitro (Fig. 3). In order to determine whether Jnk and p38 target the same serine-proline sequences in LSF as does ... See full document

... produce virus of altered tropism but would still be replication ...replication-defective virus was more restricted and the titers were reduced at least 1,000-fold compared to HIV(VSV G) pseudotypes (5, ... See full document

... wild type. (B) Packaging of viral RNA was analyzed by RT-PCR of the NL4-3mS.1 and ...NL4-3mS.3 virus stocks using a Vif-specific primer set. Helper virus (pC-Help) and NL4-3 virus ... See full document