[PDF] Top 20 Assembly, processing, and infectivity of human immunodeficiency virus type 1 gag mutants.
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Assembly, processing, and infectivity of human immunodeficiency virus type 1 gag mutants.
... The two regulatory protein coding regions for tat and rev that are essential for virus replication are intact, and transient expression of HIVgpt in COS7 cells results in assembly and re[r] ... See full document
10
Reversion of a Human Immunodeficiency Virus Type 1 Matrix Mutation Affecting Gag Membrane Binding, Endogenous Reverse Transcriptase Activity, and Virus Infectivity
... and virus assembly (39) resulting from single amino acid substitutions in ...the virus life cycle, we have obtained and characterized a viral revertant of a residue 20 mutant ...the ... See full document
10
Maintenance of the Gag/Gag-Pol Ratio Is Important for Human Immunodeficiency Virus Type 1 RNA Dimerization and Viral Infectivity
... the human immunodeficiency virus type 1 (HIV-1) Gag-Pol precursor protein results from a ⴚ 1 ribosomal frameshifting ...generates Gag and Gag-Pol in a ... See full document
8
The Mason-Pfizer Monkey Virus Internal Scaffold Domain Enables In Vitro Assembly of Human Immunodeficiency Virus Type 1 Gag
... including human immunodeficiency virus (HIV), assembly of the immature particle occurs in as- sociation with the plasma ...and assembly system for one member of this genus, Mason-Pfizer ... See full document
10
Myristoylation Is Required for Human Immunodeficiency Virus Type 1 Gag-Gag Multimerization in Mammalian Cells
... of Gag is essential for Gag-Gag multimerization in ...particle assembly do not require myristoylated Gag ...retroviral Gag proteins are stimulated to multimerize through ... See full document
12
Ubiquitination of Human Immunodeficiency Virus Type 1 Gag Is Highly Dependent on Gag Membrane Association
... Unprocessed Gag polyproteins were initially soluble in RIPA buffer but became rapidly insoluble due to multimerization and were then inac- cessible to antiserum against CA under native ...detergent-resistant ... See full document
9
Analysis of Minimal Human Immunodeficiency Virus Type 1 gag Coding Sequences Capable of Virus-Like Particle Assembly and Release
... p6 Gag deletion mutation, two consecutive stop codons were introduced into the N-terminal-coding region of the p6 gene (nucleotide [nt] ...the gag reading frame terminated at codon 449, was referred to as ... See full document
10
Role of Cellular RNA Processing Factors in Human Immunodeficiency Virus Type 1 mRNA Metabolism, Replication, and Infectivity
... RNA extraction and RT-PCR analysis. Total RNA was extracted either 48 or 72 h after transfection with the Stratagene RNA extraction kit, and DNase was treated with Turbo DNase (Ambion). Nuclear and cytoplasmic RNA ... See full document
12
Cleavage of Human Immunodeficiency Virus Type 1 Proteinase from the N-Terminally Adjacent p6* Protein Is Essential for Efficient Gag Polyprotein Processing and Viral Infectivity
... difference between particles obtained from C8166 cells (Fig. 1B, lanes 6 and 7) and those obtained from MT4 cells (lanes 2 to 5) was observed, and no PR-reactive protein in the range of 17 kDa was visible under these ... See full document
5
The C-Terminal Half of the Human Immunodeficiency Virus Type 1 Gag Precursor Is Sufficient for Efficient Particle Assembly
... To examine the ability of the D126-277 mutant to produce extracellular particles, virions released during the labeling pe- riod were pelleted through 20% sucrose and their protein composition was directly analyzed by ... See full document
5
GB virus type C E2 protein inhibits human immunodeficiency virus type 1 Gag assembly by downregulating human ADP- ribosylation factor 1
... inhibit Gag processing [17], was used as a negative control in this ...The Gag-Pol expression vector (pGPCINS) was constructed using the VR1012 vector, a CMV promoter- based expression ...the ... See full document
17
The Nucleocapsid Region of Human Immunodeficiency Virus Type 1 Gag Assists in the Coordination of Assembly and Gag Processing: Role for RNA-Gag Binding in the Early Stages of Assembly
... in assembly initiation and not a slowing in assembly progression once ...in assembly and ...NC, Gag relies on a much smaller RNA-binding site in MA for assembly (7, ...for Gag ... See full document
10
Mutation of Dileucine-Like Motifs in the Human Immunodeficiency Virus Type 1 Capsid Disrupts Virus Assembly, Gag-Gag Interactions, Gag-Membrane Binding, and Virion Maturation
... mutant Gag to be rescued upon coexpression with WT ...assay, Gag mutants are analyzed in the context of a C-terminally epitope-tagged, non- myristylated (1GA) (16) pNL4-3 ...WT Gag bearing a ... See full document
13
Reversible Binding of Recombinant Human Immunodeficiency Virus Type 1 Gag Protein to Nucleic Acids in Virus-Like Particle Assembly In Vitro
... leukemia virus) involves the selective in- corporation of viral RNA into the nascent virion despite the availability of a vast excess of cellular mRNA molecules that are also capable of supporting assembly ... See full document
6
Particle Size Determinants in the Human Immunodeficiency Virus Type 1 Gag Protein
... the assembly domains abolish or severely impair particle production, regions that lie outside these domains can be deleted without any effect on particle release or ...for assembly process play critical ... See full document
11
Influence of gag on Human Immunodeficiency Virus Type 1 Species-Specific Tropism
... in human cells because target cell CypA blocks HIV-1 ...Lv1 virus complex, as revealed by a decrease in restriction on CSA addition in simian ...a virus is insensitive to Lv1, then the effect ... See full document
7
Sequence requirements for encapsidation of deletion mutants and chimeras of human immunodeficiency virus type 1 Gag precursor into retrovirus-like particles.
... polyhedrin–HIV-1 Gag fusion protein (Gag5) have been described elsewhere (42, ...p6-sp1-deleted Gag precursor, terminated at codon 437 in the NC sequence (BglII site) and fused to the N-terminal 58 ... See full document
12
Phenotypic characterization of insertion mutants of the human immunodeficiency virus type 1 Gag precursor expressed in recombinant baculovirus-infected cells.
... The resulting phenotype of the Pr55Gag mutants expressed in the baculovirus system was characterized with respect to the following criteria: i extracellular release of membrane-enveloped[r] ... See full document
12
A Block to Human Immunodeficiency Virus Type 1 Assembly in Murine Cells
... Human immunodeficiency virus type 1 (HIV-1) does not replicate in murine ...expressed human CD4, CCR5, and cyclin T1 and contained a transactivatable HIV-1 long ... See full document
12
Target Cell Cyclophilin A Modulates Human Immunodeficiency Virus Type 1 Infectivity
... which human immunodefi- ciency virus type 1 (HIV-1) spreads in tissue ...in human CD4 ⴙ T cells, but the role of CypA in HIV-1 replication remains ...HIV-1 ... See full document
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