[PDF] Top 20 Autoregulation of Epstein-Barr virus putative lytic switch gene BZLF1.

... The distinction between the elements involved in TPA induction and the ZIIIB domain was further demonstrated by site-directed mutagenesis, which affected ZEBRA binding and transactivatio[r] ... See full document

... of lytic gene regula- tion with multiple points of ...during lytic replication and that BHRF1 and SM promoters appear to be particularly susceptible to SM-medi- ated inhibition suggests that ... See full document

... Epstein-Barr virus (EBV) is a human gamma herpesvirus which is capable of immortalizing B lymphocytes in ...the lytic cycle to various degrees in latently infected lymphocytes, including ... See full document

... EBV lytic replica- ...EBV lytic gene expression, including in the type II latency SNU719 cell line derived from an EBV-positive gastric carcinoma ... See full document

... 1217 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The product of the Epstein-Barr virus BZLF1 gene encodes a protein which is related to c-fos, it has been shown to [r] ... See full document

... The Epstein-Barr virus (EBV) proteins BZLF1 and BMRF1 are both essential for lytic EBV ...replication. BZLF1 is a transcriptional activator which binds directly to the ... See full document

... In the accompanying article, Ma et al. (39) report that upregu- lation of Zta expression is also observed after infection of whole animals with the same ZV ZV= ZIIR tmt EBV in a mouse model with a humanized immune system ... See full document

... The BZLF1 gene of Epstein-Barr virus (EBV), which encodes a transcription factor, Zta, is transcribed into monocistronic and bicistronic mRNAs from two different promoters during the ... See full document

... EBV lytic replication, two EBV genes, coding for the proteins BZLF1 and BRLF1, are expressed (8, 10, 23, 35, 36, 39, ...early gene promoters (8, 11, 14, 15, 17, 22, 32). BZLF1 (Z) is a bZIP ... See full document

... of lytic protein expres- sion and viral replication and the fact that there is no known reservoir of persistent latent infection in this lesion (1, 8, ...of BZLF1 and BRLF1 can induce lytic ... See full document

... and lytic forms of EBV infection are both essential for long-term success of the virus, it has been difficult to study the various effects of primary lytic viral infection in vitro due to the lack of ... See full document

... promoters (Fig. 2A). We found that 1 ␮ M camptothecin par- tially inhibited Zta binding at Rp ( ⬃ 28% of mock) but had a more modest effect at Hp ( ⬃ 64% of mock) or Mp ( ⬃ 72% of mock). We also tested whether Topo I ... See full document

... the BZLF1-positive and apoptotic populations were mutually exclusive was obtained by using a combination of BZLF1 immunofluorescence coupled with TUNEL staining for the products of endonuclease cleavage of ... See full document

... didate gene genetic screens, which involved crossing our GMR-Z flies to flies with known mutations in specific genes (such as tumor suppressors), and found some very interesting interactions ...EBV lytic ... See full document

... the phosphorylation statuses of three MAPKs (ERK, p38, and JNK) in the absence or presence of Zta expression. Figure 5A shows that phosphorylated (i.e., activated) forms of p44/p42 ERKs were prominently increased by Zta, ... See full document

... FIG 4 Loss of BDLF4 is responsible for the inhibition of late gene expression (A) HEK293 cells latently infected with EBV were transfected with the indicated expression vector and harvested after 96 h. After ... See full document

... Mutation of C189 does not influence the ability of Zta to interact with three ZRE sites. Initial experiments investigating the impact of C189 and C222 on the ability of Zta to bind to DNA revealed that both appeared as ... See full document

... induce lytic gene expression in EBV ⫹ B-cell lines ...IE BZLF1 or BRLF1 genes ...enhance lytic replication through direct association with R and/or R-induced alterations in Ikaros’ functional ... See full document

... To further functionally dissect the ZII domain, we con- structed a series of 2-bp mutations that span the CRE/AP-1 motif (see inset to Fig. 3). These mutations were then incor- porated into an artificial promoter ... See full document

... of BZLF1 protein when the mutation was introduced into the complete BZLF1 gene, expecting much higher expression of BZLF1 protein than from the wild-type ...in BZLF1 expression between ... See full document