[PDF] Top 20 Autorepression of Epstein-Barr Virus Nuclear Antigen 1 Expression by Inhibition of Pre-mRNA Processing

... other pre-mRNA processing appears to be strictly dependent on the context of the EBNA-1 binding sites within the gene ...for pre-mRNA processing in general that occurs ... See full document

... the mRNA of the LCL QIMR-MP or total RNA of EE346 cells was subjected to the S1 nuclease ...lanes 1 and 2), indicating that very little spliced RNA was ...contrast, pre- treatment of the RNA with ... See full document

... and mRNA accumulation ...ectopic expression of EBNA1 in non-EBV infected Burkitt lymphoma cell lines ...gene expression and chromosome organization, similar to its known function in the EBV ... See full document

... ICP27 expression is carried out through direct inter- action ...preferential nuclear export of HSV mRNAs transcribed from intronless genes ...notably, expression of ICP27 ap- pears to be an essential ... See full document

... increases expression of LMP-1, we exam- ined the ability of EBNA-3C to activate expression from the LMP-1 promoter in transient transfection ...increased expression from the ... See full document

... Epstein-Barr virus latent protein EBNA3C has been shown to bind Nm23-H1, a known suppresser of cell migration and metastasis and a regulator of the guanine exchange factor ...upregulate ... See full document

... the expression of reporter plasmids containing EBV Cp latency-associated promoter ...EBNA mRNA initiation, so it appears that EBNA3C contributes to a negative autoregulatory control ...an expression ... See full document

... with Epstein-Barr virus nuclear antigen-1 or with any other EBV protein, but forms complexes with other ...by inhibition at 10(6)-10(8) M-1, and neither ... See full document

... with Epstein-Barr virus (EBV) or closely related gammaherpesviruses in the same lymphocryptovirus (LCV) ...EBV nuclear antigen 1 (EBNA1) because of its essential role in the ... See full document

... Epstein-Barr virus (EBV) latent antigen EBNA3C is implicated in B-cell immortalization and linked to several B-cell malignan- ...H2AX expression at the transcript and protein ... See full document

... To identify the cis-regulatory sequences of the HIV-1 LTR target for EBNA2 activation, we performed cell transfection experiments in which the wild-type or mutant HIV-1 LTR plasmids carr[r] ... See full document

... i Acute transfection and expression of EBNA-2 under control of simian virus 40 or Moloney murine leukemia virus promoters resulted in increased LMP1 expression in P3HR-1-infected Burkitt[r] ... See full document

... and nuclear matrix (lane 8) ...and nuclear matrix (lane 18) fractions using OT1x. (D) LSM profiles of S(⌬1-374), M15, and M4 in living interphase cells in which chromatin had been prematurely ... See full document

... RESULTS EBNA was initially detected in isolated nuclei and chromatin from both the NC37 and Raji cell lines, using the ACIF assay and the 51Cr-CF assay 28, 29.. Studies with the 5tCr-CF [r] ... See full document

... FIG. 4. Plasmid maintenance and replication assays in C666-1 cells. (A) C666-1 cells were transfected with pCAN (negative control), OriP, or OriPE plasmid as indicated, harvested after 3 to 42 cell ... See full document

... 3, two human anti-EBNA-positive sera lanes B and C and the two monoclonal antibodies lanes D _-67K and E reacted with the partially purified 72K protein prepared from EBV genome-positive[r] ... See full document

... 415-423 0022-538X/91/010415-09$02.00/0 Copyright ©D 1991, American Society for Microbiology Epstein-Barr Virus Nuclear Antigen 2 Activates Transcription of the Terminal Protein Gene Rece[r] ... See full document

... The objectives of the experiments described here were i to construct a recombinant eucaryotic vector for the reproducible expression of EBNA2 in a wide variety of cells, ii to determine [r] ... See full document

... When Raji cells are allowed to grow to high density and most of the cells become growth arrested in the G, phase of the cell cycle, the level of detectable latent membrane protein 1 LMP1[r] ... See full document

... DNA filter binding assays and DNase I footprinting experiments showed that the intact Raji EBNA-1 protein recognized the two binding site loci in oriP and the BamHI-Q locus and no other [r] ... See full document