[PDF] Top 20 PCR Based Assay To Quantify Human Immunodeficiency Virus Type 1 DNA in Peripheral Blood Mononuclear Cells
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PCR Based Assay To Quantify Human Immunodeficiency Virus Type 1 DNA in Peripheral Blood Mononuclear Cells
... HIV-1 DNA and RNA, long-term survivors had been HIV-1 seropositive for more than 10 ...whole blood by Ficoll- Hypaque gradient centrifugation. The purified cells are either processed ... See full document
5
Performance of a Novel Human Immunodeficiency Virus (HIV) Type 1 Total Nucleic Acid Based Real Time PCR Assay Using Whole Blood and Dried Blood Spots for Diagnosis of HIV in Infants
... HIV-1 DNA test v1.5. The samples were processed first using the Amplicor HIV-1 DNA test ...CAP/CTM assay. One hundred microliters of blood was extracted using the MagNApure LC ... See full document
5
Accumulation of defective viral genomes in peripheral blood mononuclear cells of human immunodeficiency virus type 1-infected individuals.
... of virus at high multiplicities of infection (30) and of the substantially longer lifetime of nonproductively compared to productively infected ...of human immu- nodeficiency virus type ... See full document
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In vitro immunologic and virologic effects of interleukin 15 on peripheral blood mononuclear cells from normal donors and human immunodeficiency virus type 1-infected patients.
... and human immunodeficiency virus (HIV) viral load in HIV-infected patients is being actively ...(LAK) cells, gamma interferon (IFN- g ) production from HIV-positive peripheral ... See full document
6
Potent Anti-R5 Human Immunodeficiency Virus Type 1 Effects of a CCR5 Antagonist, AK602/ONO4128/GW873140, in a Novel Human Peripheral Blood Mononuclear Cell Nonobese Diabetic-SCID, Interleukin-2 Receptor γ-Chain-Knocked-Out AIDS Mouse Model
... days 1 and 2 of culture ...of cells had undergone up to 10 cycles and beyond in proliferation ...positive cells which accumulated on days 2 and 4 (Fig. 3B) were murine cells that engulfed and ... See full document
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Mutation Patterns of the Reverse Transcriptase and Protease Genes in Human Immunodeficiency Virus Type 1 Infected Patients Undergoing Combination Therapy: Survey of 787 Sequences
... HIV-1 DNA extraction and sequencing. Proviral DNA was isolated ex vivo from patients’ peripheral blood mononuclear cells with the QIAamp kit (Qiagen) without a coculture ... See full document
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Level of Human Immunodeficiency Virus DNA in Peripheral Blood Mononuclear Cells Correlates with Efficacy of Antiretroviral Therapy
... HIV DNA-containing cells, its variation in sequential samples, and its correlation with therapy ...318 blood samples were collected during a 3-year study period (1996 to 1998) from 53 HIV-infected ... See full document
5
Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... HIV PCR, then it should be called “HIV-1 not ...our assay appears to be able to identify most of the HIV-1 ...our assay also uses dUTP/ uracil DNA glycosylase to prevent ... See full document
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CD4+-T-cell counts, spontaneous apoptosis, and Fas expression in peripheral blood mononuclear cells obtained from human immunodeficiency virus type 1-infected subjects.
... expression. Cells from duplicate wells were harvested after 1, 2, or 3 days of culture, were methanol fixed, and were processed for the measurement of apoptosis by propidium iodide (PI) staining and flow ... See full document
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Comparison of Oligonucleotide Ligation Assay and Consensus Sequencing for Detection of Drug Resistant Mutants of Human Immunodeficiency Virus Type 1 in Peripheral Blood Mononuclear Cells and Plasma
... of human immunodeficiency virus type 1 (HIV-1) recede below the limit of detection of most assays applied to plasma when selective pressure is altered due to changes in ... See full document
5
Quantification of human immunodeficiency virus type 1 infected mononuclear cells in peripheral blood of seropositive subjects by newly developed flow cytometry analysis of the product of an in situ PCR assay
... The presence of human immunodeficiency virus type 1 HIV-1 proviral DNA in peripheral blood mononuclear cells PBMC of three groups group 1, more than 500 CD4+ T cells per ,ul; group 2, be[r] ... See full document
6
Multifunctional Human Immunodeficiency Virus (HIV) Gag-Specific CD8+ T-Cell Responses in Rectal Mucosa and Peripheral Blood Mononuclear Cells during Chronic HIV Type 1 Infection
... Davis. Blood was collected by sterile venipuncture using EDTA as an ...lymphoid cells for cellular immunology assays (2, 13, 23, ...and blood were processed on the same day as ... See full document
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Direct detection of proviral gag segment of human immunodeficiency virus in peripheral blood lymphocytes by colorimetric PCR assay as a clinical laboratory tool applied to different at risk populations
... (PCR)-based assay in kit form to detect directly human immunodeficiency virus type 1 (HIV-1) proviral gag sequences in peripheral blood ... See full document
7
Application of a commercial kit for detection of PCR products to quantification of human immunodeficiency virus type 1 RNA and proviral DNA
... for human immunodeficiency virus type 1 (HIV-1) RNA in plasma and proviral DNA in peripheral blood mononuclear cells (PBMC) provide valuable ... See full document
5
Resistance to Replication of Human Immunodeficiency Virus Challenge in SCID-Hu Mice Engrafted with Peripheral Blood Mononuclear Cells of Nonprogressors Is Mediated by CD8+T Cells and Associated with a Proliferative Response to p24 Antigen
... of virus in plasma are a heterogeneous group, a small subgroup of patients with truly nonprogressive human immu- nodeficiency virus (HIV) infection likely hold important clues to the basis of an ... See full document
6
Virus Burden in Human Immunodeficiency Virus Type 1-Infected Children: Relationship to Disease Status and Effect of Antiviral Therapy
... CDC, Centers for Disease Control; HIV-1, human immunodeficiency virus type 1; PBMC, peripheral blood mononuclear cell; TCID, tissue culture-infective dose.. Received for publication Dec [r] ... See full document
7
Interleukin-1 and tumor necrosis factor alpha can be induced from mononuclear phagocytes by human immunodeficiency virus type 1 binding to the CD4 receptor.
... In this study, we demonstrate that human immunodeficiency virus type 1 HIV-1 virions induce normal peripheral blood mononuclear phagocytes to produce both IL-1 and TNF within a few hours[r] ... See full document
5
Deficient Antibody-Dependent Cellular Cytotoxicity against Human Immunodeficiency Virus (HIV)-Expressing Target Cells in Perinatal HIV Infection
... ADCC against HIV-expressing CD4 lymphocytes is medi- ated by NK cells of HIV-infected adults, linked in vivo to antibodies of the subclass IgG1 (27). Such antibodies are di- rected against the viral envelope ... See full document
7
Staphylococcal exotoxin superantigens induce human immunodeficiency virus type 1 expression in naturally infected CD4+ T cells.
... Using naturally infected peripheral blood mononuclear cells depleted of CD8+ T cells, we have shown that staphylococcal exotoxins are powerful inducers of human immunodeficiency virus ty[r] ... See full document
5
Distribution and Evolution of T-Cell Receptor Vβ Repertoire on Peripheral Blood Lymphocytes of Newborn Infants of Human Immunodeficiency Virus (HIV)-Infected Mothers: Differential Display on CD4 and CD8 T Cells and Effect of HIV Infection
... Neonatal human peripheral blood mononuclear cells from 12 human immunodeficiency virus (HIV)-infected and 84 uninfected children were assessed for their ... See full document
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