[PDF] Top 20 Binding of the E1 and E2 proteins to the origin of replication of bovine papillomavirus.
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Binding of the E1 and E2 proteins to the origin of replication of bovine papillomavirus.
... the E1-ori complex on mutant oris. Binding of E1 to the ori shows an extremely nonlinear response to titra- tion of ...measure binding of E1 to the different point mutations in ori, we ... See full document
10
Induction of the Bovine Papillomavirus Origin “Onion Skin”-Type DNA Replication at High E1 Protein Concentrations In Vivo
... for E2 to stimulate E1-dependent replication ...the E1 and E2 proteins in different con- structs with different promoter strengths in the Chinese ham- ster ovary cell line CHO ... See full document
11
Isolation of an amino-terminal region of bovine papillomavirus type 1 E1 protein that retains origin binding and E2 interaction capacity.
... The bovine papillomavirus type 1 (BPV-1) E1 protein is an essential viral DNA replication factor that is both an origin recognition protein (5, 6, 13, 17, 18, 20) and an ATP-depen- dent ... See full document
5
E1 recognition sequences in the bovine papillomavirus type 1 origin of DNA replication: interaction between half sites of the inverted repeats.
... The E1 protein encoded by bovine papillomavirus type 1 (BPV-1) is required for viral DNA replication, and it binds site specifically to an A/T-rich palindromic sequence within the viral ... See full document
10
Human TATA Binding Protein Inhibits Human Papillomavirus Type 11 DNA Replication by Antagonizing E1-E2 Protein Complex Formation on the Viral Origin of Replication
... between papillomavirus replication and the process of keratinocyte differentiation has been demon- strated through in situ hybridization studies of bovine papillo- mavirus type 1 (BPV-1)-induced ... See full document
10
Mutational analysis of the 18-base-pair inverted repeat element at the bovine papillomavirus origin of replication: identification of critical sequences for E1 binding and in vivo replication.
... of binding and replication results with individual point ...wild-type origin sequence from nucleotides 7932 to 16 is shown along the x ...of E1 binding to each mutant DNA relative to ... See full document
8
Cellular Topoisomerase I Modulates Origin Binding by Bovine Papillomavirus Type 1 E1
... that E1 recruits Topo I to the PV replication fork through the interaction between the E1 C terminus and Topo I ...DNA replication, E2 and Topo I can independently stimulate ... See full document
9
Characterization of the DNA-Binding Domain of the Bovine Papillomavirus Replication Initiator E1
... between E1 and T antigen is the re- quirement for E2 for in vivo ...of E2 as an auxiliary factor in DNA replication is to interact with E1 to form the E1E2-ori ...of E1 with ori ... See full document
10
Two cellular single-strand-specific DNA-binding proteins interact with two regions of the bovine papillomavirus type 1 genome, including the origin of DNA replication.
... SPSF I and II specifically bind to single-stranded oligonucleotides containing sequences derived from one strand of the BPV-1 minimal origin of replication, from either strand of domain [r] ... See full document
11
Surface Mutagenesis of the Bovine Papillomavirus E1 DNA Binding Domain Reveals Residues Required for Multiple Functions Related to DNA Replication
... DNA replication assays for these 12 mutants are shown in ...distal E2 BS gives rise to a ...proximal E2 BS gives rise to a smaller (2.5-kb) fragment. Wild-type E1, in combination with wt ... See full document
9
DNA binding specificity of the bovine papillomavirus E1 protein is determined by sequences contained within an 18-base-pair inverted repeat element at the origin of replication.
... Additional BPV-1 specific sequences outside the 18-bp IR element did not appear to contribute significantly to El-DNA binding since the 18-mer embedded in a larger region of DNA was boun[r] ... See full document
9
The E1 Initiator Recognizes Multiple Overlapping Sites in the Papillomavirus Origin of DNA Replication
... of E1 alone, little complex formation is observed ...of E2 DBD alone, a single complex is formed ...the E1 and E2 DBDs, two major complexes form ...the E2 DBD. The second, ... See full document
11
Effect of Bovine Papillomavirus E2 Protein-Specific Monoclonal Antibodies on Papillomavirus DNA Replication
... The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and ...BPV-1 E2 protein and used them to probe the structure and ... See full document
8
Cottontail Rabbit Papillomavirus E1 and E2 Proteins Mutually Influence Their Subcellular Localizations
... The papillomavirus (PV) E2 protein is a nuclear, sequence-specific DNA- binding protein that regulates transcription and nuclear retention of viral ...genomes. E2 also interacts with the viral ... See full document
17
Two E2 binding sites alone are sufficient to function as the minimal origin of replication of human papillomavirus type 18 DNA.
... that replication of HPV-18 ori DNA by the heterologous HPV-1a E1 and E2 proteins is sensitive to the relative location of the E2BS with respect to the 16-bp IR ...the E1 and E2 ... See full document
5
Mapping and characterization of the interaction domains of human papillomavirus type 16 E1 and E2 proteins.
... of E1 may be involved in E2 interaction. Alternatively, multiple E1 confor- mational states may exist, each with its own E2 binding char- acteristics and contributory ...of E1 ... See full document
9
Functional Mapping of the DNA Binding Domain of Bovine Papillomavirus E1 Protein
... for E2 interaction support partial in vivo DNA ...some binding-defective mutants to be rescued by E2 suggested that they may retain sufficient function to support DNA ...full-length E1 in ... See full document
13
Bovine papillomavirus E1 protein binds specifically DNA polymerase alpha but not replication protein A.
... DNA replication observed in vivo by Ustav et ...transient replication assays. In these experiments, BPV E1 and E2 proteins expressed from heterologous expres- sion vectors were capable ... See full document
10
A Fifteen-Amino-Acid Peptide Inhibits Human Papillomavirus E1-E2 Interaction and Human Papillomavirus DNA Replication In Vitro
... the E2 binding site of E1. The E1-E2 interaction and peptide inhibition experiments presented here indicate functional conservation in E1-E2 rec- ognition and interaction ... See full document
8
Phosphorylation of the Bovine Papillomavirus E2 Protein on Tyrosine Regulates Its Transcription and Replication Functions
... cofactor binding, coimmunoprecipitation (co-IP) experiments were per- formed with BPV-1 E2 WT and mutant proteins and different binding partners known to enhance E2-mediated ...these ... See full document
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