[PDF] Top 20 Biochemical analysis of mutants with changes in the origin-binding domain of simian virus 40 tumor antigen.

... These activities include origin-specific and nonspecific DNA binding, melting of the imperfect palindromic sequence, untwisting of the AT-rich region, unwinding of origincontaining DNA, [r] ... See full document

... of simian virus 40 (SV40) DNA has served as a useful model system for studying initiation events in mammalian cells (for reviews, see references 11, 34, and ...well-defined origin of ... See full document

... the binding of the initial monomer, additional T-ag molecules are recruited to site I owing to the high affinity of the helicase domains for each ...helicase domain; the second strand is routed through the ... See full document

... DNA binding proteins from eukaryotic (3) and pro- karyotic organisms (6, 39) are optimal for interactions with ssDNA has been previously ...that binding of the T-Ag OBD to ssDNA is dependent upon basic ... See full document

... Simian virus 40 (SV40) T antigen is a multifunctional pro- tein and the only viral protein involved in SV40 DNA replica- ...T antigen must interact with three distinct DNA elements at ... See full document

... the origin recognition complex, cdt1, cdc6, and the minichro- mosome maintenance complex, the likely cellular replicative helicase (21, 31, ...small-DNA tumor viruses, for which the origins of replication ... See full document

... To obtain direct evidence that this region of the T antigen molecule is not only conformationally important but is, in fact, sufficient to act as the binding site with specificity for th[r] ... See full document

... Several T-antigen activities, such as origin binding, viral DNA replication, ATPase activity, stimulation of cell DNA synthesis, stimulation of rDNA transcription, transformation, and nu[r] ... See full document

... 1228 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The ATP-dependent binding of the simian virus 40 SV40 large tumor antigen T antigen to the SV40 origin of replicati[r] ... See full document

... DNA tumor viruses such as simian virus 40 (SV40), polyomavirus, or papillomavirus have become important model systems for the study of molecular events involved in DNA ...T antigen in ... See full document

... RecA (53) and the Saccharomyces cerevisiae clamp loader RFC (6). In addition, the bacterial Rho transcriptional terminator, a hexameric helicase, was recently shown to form an open-ring structure (a spiral), and the ... See full document

... of tumor suppressor proteins by the oncogene products of various DNA tumor viruses generally is considered as a means to inactivate functions of these proteins in cell cycle control (27, 42, 43, ... See full document

... The 96,000-molecular-weight anti-T-reactive surface protein was present in SV40-transforned mouse and hamster cells, as well as in productively infected monkey cells, but was not detecte[r] ... See full document

... To determine the functional significance of the SV40 lOOK T antigen in SV40 nucleoprotein complexes containing SV40 I DNA, cells were infected with SV40 tsA58, a temperature-sensitive mu[r] ... See full document

... The frequencies of transformation by six tsA-deletion double mutants and the parental tsA mutant were not significantly different when rapidly growing CHL cells were used, whether transf[r] ... See full document

... 73-82 0022-538X/82/070073-10$02.00/0 Discrete Regions of Simian Virus 40 Large T Antigen Are Required for Nonspecific and Viral Origin-Specific DNA Binding CAROL PRIVES,1* BETH BARNET,' [r] ... See full document

... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ... See full document

... Specific binding to the unmethylated 1,229-base-pair fragment was not observed, even at higher T-antigen concentrations, nor was there binding to the smaller 222-base-pair plasmid fragme[r] ... See full document

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ... See full document

... 65-76 0022-538X/83/070065-12$02.00/0 Copyright C 1983, American Society for Microbiology Simian Virus 40 Large Tumor Antigen on Replicating Viral Chromatin: Tight Binding and Localizatio[r] ... See full document