[PDF] Top 20 The Cellular TATA Binding Protein Is Required for Rep-Dependent Replication of a Minimal Adeno-Associated Virus Type 2 p5 Element
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The Cellular TATA Binding Protein Is Required for Rep-Dependent Replication of a Minimal Adeno-Associated Virus Type 2 p5 Element
... on p5-dependent ...this cellular factor could enhance the Rep-dependent replication of the minimal p5 ...a Rep-expressing construct and with (or without) a ... See full document
13
DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... nuclear replication domains, their distribution was ana- lyzed by confocal ...of Rep and ICP8 upon coinfection of wild-type AAV and HSV (10) and upon cotransfection of plasmids coding for ... See full document
5
Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System
... Rep68 protein might substitute for the HSV-1 origin binding function and direct the activity of the HSV replication complex to the AAV ...was dependent on the AAV origins of DNA ... See full document
9
Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome
... deno-associated virus type 2 (AAV2) is a nonpathogenic hu- man parvovirus with a unique biphasic life ...helper virus, AAV2 establishes a latent infection, while in the presence of a ... See full document
9
Adeno-Associated Virus Type 2 Rep Protein Inhibits Human Papillomavirus Type 16 E2 Recruitment of the Transcriptional Coactivator p300
... HPV replication and HPV-induced cellular transformation both in vivo and in ...HPV type 16 (HPV16) cel- lular transformation as well as BPV DNA replication through the activity of Rep ... See full document
9
Cellular Proteins Required for Adeno-Associated Virus DNA Replication in the Absence of Adenovirus Coinfection
... vitro adeno-associated virus (AAV) DNA replication ...system required one of the p5 Rep proteins encoded by AAV (either Rep78 or Rep68) and a crude adeno- ... See full document
11
The cellular transcription factor SP1 and an unknown cellular protein are required to mediate Rep protein activation of the adeno-associated virus p19 promoter.
... of adeno-associated virus (AAV) transcription from the three AAV promoters (p5, p19, and p40) requires the adenovirus E1a protein and the AAV nonstructural (Rep) ...The ... See full document
10
Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.
... helper virus and the virus generally used to produce ...functions required for AAV-2 or rAAV replication are probably not involved in AAV DNA synthesis ...AAV replication ... See full document
10
Inhibition of Herpes Simplex Virus Type 1 Replication by Adeno-Associated Virus Rep Proteins Depends on Their Combined DNA-Binding and ATPase/Helicase Activities
... The Rep proteins are involved in diverse processes in the viral life cycle, such as DNA replication, regulation of gene expression, ge- nome packaging, and site-specific integration (reviewed in ref- erence ... See full document
17
Adeno-Associated Virus Major Rep78 Protein Disrupts Binding of TATA-Binding Protein to the p97 Promoter of Human Papillomavirus Type 16
... AAV replication gene plays a pivotal role in the inhib- itory activity of ...not required for the inhibitory activity of ...the rep gene was truncated (p⌬KAV1). These results suggest that the ... See full document
7
Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration
... helper virus, AAV2 infection can result in a unique form of latency featuring preferential integration into the human genome at a site termed AAVS1 at human chromosome ...myosin binding subunit 85 of ... See full document
10
Differential Contribution of Adeno-Associated Virus Type 2 Rep Protein Expression and Nucleic Acid Elements to Inhibition of Adenoviral Replication in cis and in trans
... after 2 to 3 days of the amplification step ...adenoviral replication, however, obviously did not depend upon functional Rep protein expression, since an AAV genome encoding a well- ... See full document
12
Comparative Characterization of Rep Proteins from the Helper-Dependent Adeno-Associated Virus Type 2 and the Autonomous Goose Parvovirus
... AAV rep genes, four nonstructural proteins, Rep78, -68, -52, and -40, are encoded within a single open reading frame in the left half of the genome (15, 36, ...are required for virtually every aspect of the ... See full document
8
Studies of the Mechanism of Transactivation of the Adeno-Associated Virus p19 Promoter by Rep Protein
... During adeno-associated virus (AAV) type 2 productive infections, the p19 promoter of AAV is activated by the AAV Rep78 and Rep68 ...proteins. Rep-induced activation of p19 ... See full document
11
Asymmetric replication in vitro from a human sequence element is dependent on adeno-associated virus Rep protein.
... (29). Replication of AAV is dependent on the AAV nonstructural proteins (Rep); either Rep68 or Rep78 is the only AAV pro- tein required for replication in vitro (16, ...DNA ... See full document
9
Efficient Replication of Adeno-Associated Virus Type 2 Vectors: a cis-Acting Element outside of the Terminal Repeats and a Minimal Size
... cis-acting element in MVM DNA that is functionally similar to the ele- ment that we have identified in AAV2 (6, 53, ...This element was mapped to sequence positions 4489 to 4695, which is 213 bp upstream of ... See full document
11
Dynamin Is Required for Recombinant Adeno-Associated Virus Type 2 Infection
... Recombinant adeno-associated virus (rAAV) has gained in- creasing popularity for gene therapy of numerous ...certain cellular factors, such as the single- stranded DNA binding ... See full document
6
Efficient Integration of Recombinant Adeno-Associated Virus DNA Vectors Requires a p5-rep Sequence in cis
... Ad-AAV virus constructs. Specific details of the construction of Ad-Rep virus constructs used in this study, Ad-Rep210 and Ad-Rep78, can be obtained upon ...wt Rep cassette from bacterial ... See full document
11
Substitution of adeno associated virus Rep protein binding and nicking sites with human Chromosome 19 sequences
... DNA replication in plasmid-transfected cells was analyzed by using the Southern blotting ...wild type ITRs is shown in Figure ...AAV2 replication in cells transfected with pSub201(-), pVM108 or ... See full document
11
Adeno-Associated Virus Rep Represses the Human Integration Site Promoter by Two Pathways That Are Similar to Those Required for the Regulation of the Viral p5 Promoter
... the p5 RBS. Transcript and protein levels from 3 independent experiments were quantitated ...and p5 promoter activities ...and p5 promoter transcripts ...of Rep with the RBS is not ... See full document
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