[PDF] Top 20 Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
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Characterization of T antigen in cells infected with a temperature-sensitive mutant of simian virus 40.
... The present study demonstrates that a molecular form of T antigen induced by ts 900, a transformation-defective ts mutant of SV40 29, is more heat labile than that induced by WT virus, i[r] ... See full document
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Archetype JC Virus Efficiently Replicates in COS-7 Cells, Simian Cells Constitutively Expressing Simian Virus 40 T Antigen
... COS-7 cells, simian cells transformed with an origin-defective mutant of simian virus 40 ...COS-7 cells. However, when the parental cells of COS-7 not ... See full document
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ... See full document
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Detection and characterization of multiple forms of simian virus 40 large T antigen.
... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ... See full document
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Characterization of am404, an amber mutation in the simian virus 40 T antigen gene.
... 3, COS-1 cells were infected with am404 virus stock and labeled with 32p;, and the resulting protein extracts were immunoprecipitated and analyzed in an SDS-polyacrylamide gel Fig.. Immu[r] ... See full document
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Association of Simian Virus 40 T Antigen with Simian Virus 40 Nucleoprotein Complexes
... To determine the functional significance of the SV40 lOOK T antigen in SV40 nucleoprotein complexes containing SV40 I DNA, cells were infected with SV40 tsA58, a temperature-sensitive mu[r] ... See full document
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A simian virus 40 dl884/tsA58 double mutant is temperature sensitive for abortive transformation.
... Our major conclusions are that i although the mutant tsA58 is not temperature sensitive for the maintenance of transformation, the d18841tsA58 double mutant is; ii small t antigen can pr[r] ... See full document
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Genetic Analysis of Simian Virus 40 III. Characterization of a Temperature-Sensitive Mutant Blocked at an Early Stage of Productive Infection in Monkey Cells
... The mutant virion can adsorb to and penetrate the cell normally at the restrictive temperature, but cannot induce the synthesis of cellular deoxyribonucleic acid DNA nor initiate the syn[r] ... See full document
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Temperature-Sensitive Mutants of Simian Virus 40: Infection of Permissive Cells
... Plaque characteristics of wild-type and mutant simian virus 40 SV40 stocks propagated at 32 C Virus Efficiency of plating39 C/32 C Time of plaque appearance in days d Infected cultures w[r] ... See full document
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Oligomerization of simian virus 40 large T antigen is not necessarily repressed by temperature-sensitive A gene lesions.
... tsA1499-infected cells were kept for 96 h at 32 or 41°C, radiolabeled with [32P]phosphate, extracted, and analyzed for both T-p53 complexes and free T antigen by sequential immunoprecipi[r] ... See full document
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Temperature-Sensitive Simian Virus 40 Mutant Defective in a Late Function
... To determine whether mutant SV40tsTNG-1 also induced cellular DNA synthesis, 5-day-old cultures of primary mouse kidney cells 3.3 X 106 cells per culture were infected at input multiplic[r] ... See full document
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Antigenic structure of simian virus 40 large tumor antigen and association with cellular protein p53 on the surfaces of simian virus 40-infected and -transformed cells.
... The anti-T-ag monoclonal antibodies coprecipitated Several of the recognized biological activities of T-ag have the 53K cellular protein pS3 from the surface of SV40been assigned to rela[r] ... See full document
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Relationship between T-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells.
... 1 Relationship Between T-Antigen and TumorSpecific Transplantation Antigen in Simian Virus 40Transformed Cells Received for publication 18 July 1978 The simian virus 40 SV40 tumor antige[r] ... See full document
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Recombination Between Endogenous and Exogenous Simian Virus 40 Genes. I. Rescue of a Simian Virus 40 Temperature-Sensitive Mutant by Passage in Permissive Transformed Monkey Lines
... Rescue of a Simian Virus 40 Temperature-Sensitive Mutant by Passage in Permissive Transformed Monkey Lines Received for publication 11 May 1977 Passage of the simian virus 40 SV40 temper[r] ... See full document
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Functional analysis of a simian virus 40 super T-antigen.
... We have not compared the properties of the super and large T-antigens in the maintenance of transformation, but noted that cells transformed by the super T-antigen templates and the C120[r] ... See full document
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Structure and synthesis of a simian virus 40 super T-antigen.
... DISCUSSION In productively infected permissive cells, the early region of SV40 DNA is transcribed into two mRNAs of 2.6 and 2.3 kb, which encode proteins of apparent molecular weights 17[r] ... See full document
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Cytoplasmic T antigens of mouse and human cells transformed by a simian virus 40 tsA mutant.
... T antigens were demonstrated by indirect immunofluores782 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Simian virus 40 T antigens accumulate in the cytoplasm of simi[r] ... See full document
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Mutational analysis of simian virus 40 small-t antigen.
... 2895 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Several point mutations in the simian virus 40 SV40 small-t antigen have been analyzed for their effects on protein[r] ... See full document
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Simian virus 40 T- and U-antigens: immunological characterization and localization in different nuclear subfractions of simian virus 40-transformed cells.
... Recently, biochemical and immunological evidence has been provided demonstrating that in HeLa cells infected by different Ad2+-SV40 hybrid viruses, SV40-specific proteins carrying SV40 U[r] ... See full document
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Activation of simian virus 40 transcription in vitro by T antigen.
... 4591 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Simian virus 40 is repressed when the viral early gene product large tumor antigen TAg binds to specific sites withi[r] ... See full document
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