[PDF] Top 20 Characterization and classification of virus particles associated with hepatitis A. III. Structural proteins.

... RESULTS Electrophoresis of the proteins of mature poliovirus particles disrupted by boiling and radioiodinated in vitro revealed the well-known pattern 14 of the four structural polypept[r] ... See full document

... Type and Configuration of Nucleic Acid Received for publication 17 October 1977 Virus particles banding at 1.34 g/ml in CsCl and sedimenting at 160S in sucrose gradients were isolated fr[r] ... See full document

... In the present study the characteristics of virus-like particles in stools collected from patients in an epidemic of hepatitis A in Germany 10 were determined and compared with those of [r] ... See full document

... model, hepatitis C virus (HCV) can be efficiently propagated in cell ...HCV structural proteins in the context of an infectious ...HCV structural proteins indicated that, in ... See full document

... of hepatitis C virus (HCV) RNA-containing particles in the low-density fractions of plasma has been associated with high ...HCV particles and their association with immunoglobulins or ... See full document

... HCV structural pro- teins in the baculovirus expression system (3) or by Semliki Forest virus replicons ...these particles were not secreted into the ...Forest virus expres- sion systems ... See full document

... Hepatitis C virus (HCV) nonstructural protein 5A (NS5A) exhibits a preference for G/U-rich RNA in vitro. Biological analysis of the NS5A RNA-binding activity and its target sites in the genome will be ... See full document

... In serum, the pre-S proteins were shown to exist predominantly in DHBV-DNA-free particles associated with a 17-kilodalton protein which, by N-terminal amino acid sequence analysis, was s[r] ... See full document

... HCV particles, we incubated them with a well-characterized E2- specific neutralizing antibody (AR3A) (36) known to specifi- cally immuno-deplete viral infectivity and immunoprecipitate HCV RNA from infectious ... See full document

... membrane-associated hepatitis E virus (HEV) particles—now considered “quasi-enveloped particles”—are present in the multivesicular body with intraluminal vesicles (exosomes) in infected ... See full document

... HCV structural proteins are detectable in infected cell cul- ture ...increase virus release ...Secreted virus was pelleted by ultracentrifu- gation in order to concentrate the virions and to ... See full document

... E2 proteins, with and without p7, have led to the suggestion that p7 may play a role in glycoprotein maturation or virus morphogenesis (8, 30, ...major structural component of the virion ...HCV-like ... See full document

... are consistent with the diagram presented in panel D. (B) Locations and orientations of the primers relative to nonrearranged HBV integrant, which is the likely template for the synthesis of HBV RNAs producing the L, M, ... See full document

... E virus (HEV) is a spherical, nonenveloped virus around 27 to 34 nm in diameter, whose genome is a single- stranded RNA molecule of positive polarity and approximately ... See full document

... Figure 3 shows in addition that purified virus that was dialyzed against the pH 10.6 carbonate buffer in the presence of the reducing RESULTS agent DTT, conditions which are known to dis[r] ... See full document

... Titration curves of an equivalent vol- Between pH 5.7 and 3.0, therefore, the number of titratable groups of virus particles per 100 mol ume of 0.1 M KC0 were obtained, and the number of[r] ... See full document

... these ORFs within their respective genomes is not conserved between the two viruses. HCMV pUL25 also has a short region (residues 289 to 399) which shares a 21.4% amino acid se- quence identity with HSV-1 UL9 gene ... See full document

... A virus at the plasma membrane of infected cells leads to release of enveloped virions that are typically round in tissue culture-adapted strains but filamentous in strains isolated from ...viral proteins ... See full document

... envelope proteins of HBV, respectively (4, ...(4). Virus particles and SVP were concentrated 100-fold using polyethylene glycol (PEG) precipitation (17) and then resuspended in STE ...with ... See full document

... The M protein of HPIV3 has a molecular weight of 35 x 1 is associated with the envelope of the virion, and aggregates under low-salt conditions, and intrachain disulfide bonds affect its[r] ... See full document