[PDF] Top 20 Characterization of the gene encoding herpes simplex virus type 2 glycoprotein C and comparison with the type 1 counterpart.

... To obtain the complete nucleotide sequence of the region of the HSV-2 genome encoding gC, the protein encoded by the smaller 3'coterminal message, and the upstream and downstream regulat[r] ... See full document

... in type- specific gG-1 ELISA were drawn from patients harboring MAb-negative ...4. Comparison of OD values in ELISA for sera drawn from patients harboring MAb-negative or MAb-positive isolates using ... See full document

... recombinant virus, incubated at 37°C, harvested at 18 h after infection, solubilized in disruption buffer, electrophoretically separated in a denaturing polyacrylamide gel, transferred to a nitrocel- lulose ... See full document

... containing 2% Triton X-100, 300 mM NaCl, and ...in 2⫻ CLB (see Materials and Methods) followed by ...contained 2% Triton X-100 and a high salt concentration, and this treatment solubilized and ... See full document

... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document

... UL56 gene product of herpes simplex virus (HSV) has been shown to play an important role in viral ...HSV type 2 (HSV-2) and examined its expression and ...The gene ... See full document

... gG-1 gene in clinical isolates is currently lacking, data on the genetic variability of other HSV glycoproteins are ...In comparison to the minor sequence alterations reported in these studies, the ... See full document

... the C ter- minus of the truncated gene with a polylinker in the expression ...and 2% ...and characterization of HSV gB2t will be published ...anti-HSV type 2 polyclonal ...the ... See full document

... gD gene of interest using the Invitrogen ...in 1 M NaCl in a sterilized eppendorf tube with incubation on ice for 30 ...for 1 second to mix and then incubated at 65°C for 6 ... See full document

... However, a search for homology at the amino acid level showed regions of significant sequence similarity between the amino acids of the carboxy half of EHV-1 gp13 and those of the same r[r] ... See full document

... Effect of endo H on newly synthesized and immunoprecipitation with anti-gF-2 111188 processed forms of gF-2 immunoprecipitated monoclonal antibody followed by preparative anti-gF-2 III18[r] ... See full document

... Analysis of the potential coding sequences within the 2.9-kb Sall DNA fragment of HSV-2 revealed an open reading frame of 479 amino acids which began with the methionine encoded at posit[r] ... See full document

... Anatomy of the herpes simplex virus type 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants.. [r] ... See full document

... While both genes are not absolutely required for virus growth in tissue culture 5, 14, 19, PRV glll is known to be important for efficient adsorption to or entry into cells 16 and for ef[r] ... See full document

... We have found that, as in PrV, a portion of an upstream open reading frame exhibiting extensive nucleotide and amino acid sequence similarity 58.9% similarity over 175 amino acids to the[r] ... See full document

... To determine more specifically whether the rates of synthesis of ICPO, ICP27, and ICP6 in d120- and d202-infected cells differ from that in tsB32-infected cells and to examine the possib[r] ... See full document

... Viruses isolated in this study Virus Phenotype' Parent virus Comments Donor fragment A4B WT Fl gB PCR fragment from 17syn+ A61B WT Fl gB PCR fragment from 17syn' 1ACSS C4A WT Syn Syn Syn[r] ... See full document

... If the coprecipitation of the pKAlB polypeptide in the presence of pK230 is due to interactions between two upstream sites, then the upstream site is localized to amino acid residues 235[r] ... See full document

... 818 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Hybrid arrest of in vitro translation was used to localize the region of the herpes simplex virus type 1 genome enco[r] ... See full document

... Because heparan sulfate moieties of cell surface proteoglycans serve as the receptors for herpes simplex virus adsorption, we tested whether any of the viral glycoproteins could bind to [r] ... See full document