[PDF] Top 20 Characterization of hepatitis delta antigen: specific binding to hepatitis delta virus RNA.
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Characterization of hepatitis delta antigen: specific binding to hepatitis delta virus RNA.
... HDV genomic RNA from the plasma of an HDV-infected patient was prepared by disrupting hepatitis delta virions purified from the patient's serum with the lysis buffer containing 80 mM Tri[r] ... See full document
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Arginine-Rich Motifs Are Not Required for Hepatitis Delta Virus RNA Binding Activity of the Hepatitis Delta Antigen
... of binding observed by Lee et ...required RNA binding ...for binding specificity (37), but there is no way of knowing in the previous study by Lee et ...cific binding activity, such as ... See full document
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Identification and characterization of a hepatitis delta virus RNA transcriptional promoter.
... Hepatitis delta virus (HDV) is a defective virus that is de- pendent on concurrent infection with hepatitis B virus for replication and ...circular RNA molecule of ...HDV ... See full document
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Genotype-Specific Complementation of Hepatitis Delta Virus RNA Replication by Hepatitis Delta Antigen
... HDV RNA replication ...the virus life ...of hepatitis B virus and woodchuck hepatitis virus, which infect different hosts, have been shown to be functionally in- terchangeable ... See full document
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Transcription of Subgenomic mRNA of Hepatitis Delta Virus Requires a Modified Hepatitis Delta Antigen That Is Distinct from Antigenomic RNA Synthesis
... qRT-PCRs specific for HDV G-RNA, AG- RNA, and HDAg-encoding ...HDV RNA species using a previ- ously established HDV cDNA-free RNA transfection system ...dependent RNA ... See full document
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The Conserved Serine 177 in the Delta Antigen of Hepatitis Delta Virus Is One Putative Phosphorylation Site and Is Required for Efficient Viral RNA Replication
... HDV RNA replication, although there are other ...HDV RNA and forms the ...of virus is a ...genomic RNA and viral polymerase in an unphosphorylated manner to form a core particle ...acid ... See full document
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Multimerization of Hepatitis Delta Antigen Is a Critical Determinant of RNA Binding Specificity
... Hepatitis delta virus (HDV) RNA forms an unbranched rod structure that is associated with hepatitis delta antigen (HDAg) in cells replicating ...vitro binding ... See full document
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Characterization of Hepatitis Delta Virus in Sub Saharan Africa
... HDV) RNA par- ticles may, in contrast to HBV, assemble even in the absence of large HBsAg (24, 41), it was not unexpected that HBV DNA was undetectable in almost 50% (11/23) of the HDV RNA-positive ... See full document
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Hepatitis delta antigens enhance the ribozyme activities of hepatitis delta virus RNA in vivo.
... the RNA-binding activity of ...HDV RNA, probably recognizing a secondary structure unique to HDV RNA rather than specific HDV nucleotide sequences (8, 18, ...the binding of HDAg ... See full document
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Characterization of the Phosphorylated Forms and the Phosphorylated Residues of Hepatitis Delta Virus Delta Antigens
... Hepatitis delta virus (HDV) replication requires both the cellular RNA polymerase and one virus-encoded protein, small delta antigen ...large delta antigen ... See full document
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Hepatitis Delta Antigen Requires a Flexible Quasi-Double-Stranded RNA Structure To Bind and Condense Hepatitis Delta Virus RNA in a Ribonucleoprotein Complex
... the RNA binding properties of HDAg-160 are reminiscent of nucleic acid binding proteins that bend dsDNA ...but binding affinity is affected by the flexibility of the nucleic acid (41, ... See full document
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ERK1/2-Mediated Phosphorylation of Small Hepatitis Delta Antigen at Serine 177 Enhances Hepatitis Delta Virus Antigenomic RNA Replication
... HDV RNA in double-rolling-circle ...antigenomic RNA to genomic RNA syn- thesis (14, 29, 32, 37, ...vitro RNA-dependent RNAP activity in HDV antigenomic RNA-derived scaffolds (14, 31) at ... See full document
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Hepatitis Delta Virus Replication Generates Complexes of Large Hepatitis Delta Antigen and Antigenomic RNA That Affiliate with and Alter Nuclear Domain 10
... genomic RNA-specific probe that will hybridize to nucleotides 38 to 82 of the HDV genome (5⬘-CGTTCCAATGCT CTTTACCGTGACATCCCCTCTCGGGAGCTGATC-3⬘); (ii) an antigenomic RNA-specific probe that ... See full document
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Transcription of Hepatitis Delta Antigen mRNA Continues throughout Hepatitis Delta Virus (HDV) Replication: a New Model of HDV RNA Transcription and Replication
... was specific for genotype I (nt 1565 to 1591), it detected the genotype I mRNA (100% complementarity to nt 1565 to 1591) (lane 3) but not the genotype I/II chimeric mRNA (55% complementarity to nt 1565 to 1591) ... See full document
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Transcription of Hepatitis Delta Virus RNA by RNA Polymerase II
... the RNA genome of hepatitis delta virus (HDV) involves redirection of RNA polymerase II (Pol II), a host enzyme that normally uses DNA as a ...HDV RNA transcription, a polymerase ... See full document
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RNA-binding activity of hepatitis delta antigen involves two arginine-rich motifs and is required for hepatitis delta virus RNA replication.
... To determine whether either of the ARM-like sequences was involved in RNA binding, we made a series of deletion mutants starting from the C-terminal end of the middle domain M of HDAg am[r] ... See full document
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Hepatitis Delta Antigen Requires a Minimum Length of the Hepatitis Delta Virus Unbranched Rod RNA Structure for Binding
... Previous binding studies, which used denatured bacterially expressed HDAg, were qualitative, and could not distinguish binding character- istics of different ...that binding to different unbranched ... See full document
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The antigen of hepatitis delta virus: examination of in vitro RNA-binding specificity.
... We found that the ability of the HDV genomic and antigenomic RNAs to fold into the rodlike structure was an essential feature for specific RNA binding, and we were able to detect a funct[r] ... See full document
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Hepatitis Delta Antigen Mediates the Nuclear Import of Hepatitis Delta Virus RNA
... the RNA-binding motifs of HDAg (26), were constructed by replacing the SphI-StuI fragment of pGEX-Sm with the corresponding fragments from pECE-A1 and pECE-A29, respectively (26); the C termini of the ... See full document
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Functional motifs of delta antigen essential for RNA binding and replication of hepatitis delta virus.
... Lanes W, M, N, and MN contain the wild-type and mutants which are mutated in the middle domain, near the N terminus, or both of the leucine repeats for the small HDAg, respectively.. Pla[r] ... See full document
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