[PDF] Top 20 Characterization of Salmonella Serovars by PCR Single Strand Conformation Polymorphism Analysis
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Characterization of Salmonella Serovars by PCR Single Strand Conformation Polymorphism Analysis
... these serovars (Entrez-Pubmed Blast Search [http: ...other Salmonella serovars are not ...some serovars and is more relaxed in others? One reason could be that the mutations detected do not ... See full document
6
Genotypic detection of Mycobacterium tuberculosis rifampin resistance: comparison of single strand conformation polymorphism and dideoxy fingerprinting
... Cost analysis of ddF versus automated DNA ...the PCR and automated sequencing machines common to both ...data analysis times were assumed to be the same for both ... See full document
7
Rapid Identification of Bacteria from Positive Blood Cultures by Fluorescence Based PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... the analysis of additional peaks for two or more organisms could become difficult, it was actually quite easy to interpret, as seen for 11 of our mixed-culture ... See full document
8
Identification of Mycobacterium Species by Multiple Fluorescence PCR–Single Strand Conformation Polymorphism Analysis of the 16S rRNA Gene
... SSCP analysis patterns were exported to a software program, BioNumerics, version ...the analysis of the results of SSCP ...primer-labeled PCR product, were entered into a database as a separate ... See full document
7
Capillary Electrophoresis–Single Strand Conformation Polymorphism Analysis for Rapid Identification of Pseudomonas aeruginosa and Other Gram Negative Nonfermenting Bacilli Recovered from Patients with Cystic Fibrosis
... electrophoresis–single-strand conformation polymorphism (CE-SSCP) analysis of PCR- amplified 16S rRNA gene fragments for rapid identification of Pseudomonas aeruginosa and other ... See full document
6
Multiple infections of Ixodes scapularis ticks by Borrelia burgdorferi as revealed by single strand conformation polymorphism analysis
... the PCR product. The amount of PCR product used depended on the brightness of the band when the PCR product was run on an agarose ...best strand separation, the gel was run for approximately ... See full document
5
Molecular identification of bacteria by fluorescence based PCR single strand conformation polymorphism analysis of the 16S rRNA gene
... multiplex PCR-SSCP. Mul- tiplex PCR-SSCP (15) makes a further differentiation of spe- cies which show conserved SSCP patterns with a PCR with a single primer ...Also, PCR-SSCP with ... See full document
6
PCR and single strand conformational polymorphism for recognition of medically important opportunistic fungi
... conformational polymorphism analysis is performed by electrophoresis of the amplicon from the PCR on a polyacrylamide gel, thus eliminating the need for restric- tion ...SSCP analysis displays ... See full document
5
Novel Algorithm Identifies Species in a Polymycobacterial Sample by Fluorescence Capillary Electrophoresis Based Single Strand Conformation Polymorphism Analysis
... (FCE)-based single-strand conformation polymorphism (SSCP) analysis was ...was PCR amplified with a fluorescence-labeled mycobacterium-specific primer, ... See full document
8
Detection of rifampin resistance by single strand conformation polymorphism analysis of cerebrospinal fluid of patients with tuberculosis of the central nervous system
... FIG. 2. PCR-SSCP patterns obtained by processing CSF samples from pa- tients with CNS-TB. Lanes S, pattern obtained from rifampin-sensitive M. tu- berculosis reference strain (ATCC 27294). Samples (lanes) 1 to 3, ... See full document
5
Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR based mutation scanning approach
... genus. PCR-based tools employing various nuclear ...quantitative PCR (qPCR), combined with single-strand conformation polymorph- ism (SSCP)-based sequencing analysis, was used ... See full document
5
A single strand conformation polymorphism study of CD40 ligand Efficient mutation analysis and carrier detection for X linked hyper IgM syndrome
... of PCR primers that could be used to screen genomic DNA by single strand conformation polymorphism (SSCP) were ...SSCP analysis permitted rapid carrier detection in two families ... See full document
7
Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR–Single Strand Conformation Polymorphism Analysis
... 14 serovars (22). As shown earlier by DNA hybridization studies on serovars 1 to 8, these can be divided into two clusters or ...fragment analysis (7) and 16S rRNA se- quencing (20), and genome size ... See full document
5
Detection of Rifampin Resistant Mycobacterium tuberculosis in Sputa by Nested PCR Linked Single Strand Conformation Polymorphism and DNA Sequencing
... nested PCR-SSCP analysis for sputa. When con- ventional PCR-SSCP using the TR9-TR8 primer set was di- rectly applied to sputa, rifampin susceptibility of 11 of 56 sam- ples could not be determined ... See full document
8
Genetic Relatedness of Salmonella Serovars Isolated from Catfish (Clarias gariepinus) and Tilapia (Tilapia mossambica) Obtained from Wet Markets and Ponds in Penang, Malaysia
... Germany). PCR was performed as previously described by Szczuka and Kaznowski (29), with modifications of the denaturation, annealing, elongation, and final extension ...the PCR product was mixed with 1 ll ... See full document
7
The epidemiology of Salmonella serovars in Tasmania
... APPENDICES Appendix 1 Media recipes Appendix 2 Wild fauna examined for Salmonella Appendix 3 Salmonella serovars isolated from humans in Tasmania Appendix 4 Salmonella serovars isolated [r] ... See full document
166
Genetic Diversity among Borrelia Strains Determined by Single Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness
... Wang et al. (24) have shown that the genetic diversity of B. burgdorferi sensu stricto within a local population of ticks is almost equal to the worldwide genetic diversity. Our study revealed that ospC groups identified ... See full document
7
Combined PCR oligonucleotide ligation assay for rapid detection of Salmonella serovars
... of Salmonella CFU or with known concentrations of purified chromosomal DNA from Salmonella typhimurium ATCC 29946, the PCR-OLA was able to detect > 20 CFU per assay or > 80 fg of chromosomal ... See full document
6
Nucleation of polymorphic amyloid fibrils
... fibril polymorphism is relevant biologically, because different fibril polymorphs can have different toxicity, but a predictive tool for which polymorph forms and under what conditions is ...fibrils, ... See full document
21
Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections
... of Salmonella serovar Typhi in the sera of individuals with suspected enteric fever ...a single, acute-phase serum sample is often used, but false-negative and false-positive results are ... See full document
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