[PDF] Top 20 Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV 1 subtype B and subtype C
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Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV 1 subtype B and subtype C
... on HIV-1 subtype B and C RT, recombinant RT heterodimers were analyzed in cell-free RNA-depen- dent DNA polymerase assays in the presence of NRTIs ZDV-TP, 3TC-TP, and TFV-DP, or ... See full document
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Effects of the K65R and K65R/M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance
... The subtype B HIV-1 RT expression plasmid pRT6H-PROT [24] was kindly provided by ...Grice. Subtype B RTs containing mutations K65R and K65R+M184V were generated as described ... See full document
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Sequence and drug susceptibility of subtype C reverse transcriptase from human immunodeficiency virus type 1 seroconverters in Zimbabwe.
... (Table 1), Taq polymerase, and dye-labelled dideoxy terminators (Applied Biosystems, ...examined. HIV-1 env heteroduplex mobility assay analysis. HIV-1 proviral DNA from cultured ... See full document
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Simultaneous Detection of Major Drug Resistance Mutations in the Protease and Reverse Transcriptase Genes for HIV 1 Subtype C by Use of a Multiplex Allele Specific Assay
... l 1 ⫻ Tm hy- bridization buffer containing 4 g/ml streptavidin R-phycoerythrin (Mo- lecular Probes, Eugene, OR) and incubated at 37°C for 15 ...Figure 1 is a schematic illustration of the mASPE and ... See full document
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Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility
... treat HIV infection, the phenotypic susceptibility analysis showed that diversity within group M might affect drug ...variants. HIV-1 group O isolates are naturally resistant to nonnucleoside ... See full document
5
Pol-Driven Replicative Capacity Impacts Disease Progression in HIV-1 Subtype C Infection
... reduce HIV-1 replica- tion capacity (RC) and alter disease progression, but less is known about immune- mediated attenuation in other HIV-1 ...487 recombinant viruses encoding ... See full document
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Genetic Diversity of Protease and Reverse Transcriptase Sequences in Non Subtype B Human Immunodeficiency Virus Type 1 Strains: Evidence of Many Minor Drug Resistance Mutations in Treatment Naive Patients
... Phylogenetic and sequence analyses. The genetic subtypes were determined by phylogenetic tree analysis. The new nucleotide sequences and the sequences of the protease and RT genes from reference strains ... See full document
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Augmentation of Human Immunodeficiency Virus Type 1 Subtype E (CRF01_AE) Multiple-Drug Resistance by Insertion of a Foreign 11-Amino-Acid Fragment into the Reverse Transcriptase
... type 1 (HIV-1) subtype E (CRF01_AE) variant (99JP-NH3-II) possessing an in-frame 33-nucleotide insertion mutation in the  3-  4 loop coding region of the reverse transcriptase ... See full document
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Subtype associated differences in HIV 1 reverse transcription affect the viral replication
... backbone subtype C vector for recombinant clones, complete 1084i provirus was excised from the parental ...restriction enzymes were from New England Biolabs) and sub- cloned into the same ... See full document
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Performance of Applied Biosystems ViroSeq HIV 1 Genotyping System for Sequence Based Analysis of Non Subtype B Human Immunodeficiency Virus Type 1 from Uganda
... ViroSeq HIV-1 Genotyping System is a commercially available, integrated system for sequence-based analysis of drug resistance mutations in human immunodeficiency virus type 1 ... See full document
5
Comparative biochemical analysis of HIV 1 subtype B and C integrase enzymes
... of 1 mM. The cultures were incubated for 3 hours at 37°C, centrifuged (5000 rpm for 12 min), and frozen at ...for 1 hour at 4°C with mild ... See full document
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Different Effects of Nonnucleoside Reverse Transcriptase Inhibitor Resistance Mutations on Cytotoxic T Lymphocyte Recognition between HIV-1 Subtype B and Subtype A/E Infections
... with subtype A/E ...antiretroviral-naive HIV-1-infected individuals, we analyzed 363 FIG 3 Effect of RT181 mutations on HLA-B*35:01-restricted NY9-specific CTL recognition in subtype ... See full document
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Subtype-Specific Conformational Differences within the V3 Region of Subtype B and Subtype C Human Immunodeficiency Virus Type 1 Env Proteins
... for subtype B HIV-1, on whether the virus is R5 tropic or X4 ...with subtype C HIV-1 (1, 4, 7, 8, 13, 15, 41, 54, ...a subtype B R5-tropic ... See full document
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Co-receptor tropism prediction among 1045 Indian HIV-1 subtype C sequences: Therapeutic implications for India
... in subtype C populations; this method has an estimated sensitivity of ...African subtype C strains reported 100% concordance of C-PSSM with phenotypic assay in detecting CXCR4 ...in ... See full document
6
A Reliable Phenotype Predictor for Human Immunodeficiency Virus Type 1 Subtype C Based on Envelope V3 Sequences
... 279 HIV-1 subtype C V3 sequences was compiled from published literature, the Entrez nucleotide database ...Alamos HIV sequence database ...phenotyped subtype C sequences ... See full document
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Human Immunodeficiency Virus Type 1 Env Sequences from Calcutta in Eastern India: Identification of Features That Distinguish Subtype C Sequences in India from Other Subtype C Sequences
... type 1 (HIV-1) infection has been reported in more than 173 countries worldwide ...spread, HIV-1 infections were mainly found in North America, western Europe, and sub-Saharan ...While ... See full document
9
Molecular characterisation of hepatitis B virus in HIV 1 subtype C infected patients in Botswana
... Some diagnostic kits have difficulty detecting some genotypes like Genotype D and some S gene mutants; therefore, it is important to know the molecular characteristics of the circulating[r] ... See full document
9
UCLA1, a Synthetic Derivative of a gp120 RNA Aptamer, Inhibits Entry of Human Immunodeficiency Virus Type 1 Subtype C
... against HIV-1 subtype C ...for HIV-1 ConC gp120 and broad neutralization of primary isolates and Env-pseu- dotyped ...inhibit HIV-1 when combined with the ap- ... See full document
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Profiling the Specificity of Neutralizing Antibodies in a Large Panel of Plasmas from Patients Chronically Infected with Human Immunodeficiency Virus Type 1 Subtypes B and C
... polyclonal HIV Ig. For adsorptions, plasmas were diluted to between 1:20 to 1:30 in Dulbecco’s modified Eagle’s medium (DMEM)–10% fetal bovine serum and 800 to 1,000 l of diluted plasma was ... See full document
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Host-Specific Adaptation of HIV-1 Subtype B in the Japanese Population
... in HIV-1-specific T cells elicited between the Japanese and Caucasian cohorts, possibly as a result of differences in host genetics (for example, in the genes that encode the T-cell receptor and/or modulate ... See full document
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