[PDF] Top 20 Comparative Evaluation of PCR with Commercial Multiplex M tuberculosis Detection Kit
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Comparative Evaluation of PCR with Commercial Multiplex M tuberculosis Detection Kit
... the PCR positivity was found 100% in smear positive samples by utilizing In House PCR as well as commercial multiplex ...M. tuberculosis PCR ...of M. ... See full document
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Comparative Evaluation of Several Gene Targets for Designing a Multiplex PCR for an Early Diagnosis of Extrapulmonary Tuberculosis
... plex PCR, with the exception of the different primer pair con- ...of M-PCR, the combinations of different concentrations of two primer pairs were evaluated (Table 2) using 1 μL of purified ... See full document
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Comparative evaluation of a laboratory developed real time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
... virus PCR Kit which is also 100% specific when tested against 35 different viral and bacterial agents ...the commercial kit which has been evaluated for the seven species (A to G) of HAdV, our ... See full document
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Comparative Evaluation of Two Commercial Multiplex Panels for Detection of Gastrointestinal Pathogens by Use of Clinical Stool Specimens
... The detection of pathogens associated with gastrointestinal disease may be important in certain patient populations, such as immunocompromised hosts, the critically ill, or individuals with prolonged disease that ... See full document
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... mRT- PCR from the state of Chhattisgarh, a central region of India ...its comparative evaluation with GeneXpert, conventional microscopy and liquid culture MGIT 960 system in the diagnosis of ... See full document
5
Relevance of Commercial Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Clinical Samples
... the kit according to and without modifying the manufacturer’s ...a commercial kit recommended for respiratory specimens, Palacios et ...6. Evaluation of the LCx assay for detection of ... See full document
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Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples
... MT-PCR. (i) Primer design. To determine suitable areas for primer produc- tion, consensus sequences were generated from available GenBank sequences for each species. The sequence data from different species were ... See full document
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Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... isolation kit I (Roche, Indianapolis, IN), according to the manufacturer’s recommendations for the MagNA Pure LC automated nucleic acid extraction ...conventional PCR for HAdV-B3, -E4, -B7, -B11, -B14, or ... See full document
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Rapid and Simultaneous Detection of Mycobacterium tuberculosis Complex and Beijing/W Genotype in Sputum by an Optimized DNA Extraction Protocol and a Novel Multiplex Real Time PCR
... m in diameter) with a silica-like surface to capture nucleic ...spin kit (Qiagen) utilizes carrier RNA to enhance binding of nucleic acids to silica membrane, which may be particularly useful if there are ... See full document
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Detection of Escherichia coli Pathotypes from the Cases of Diarrhea
... three multiplex PCR assays. The PCR assays detected 26 ETEC, 20 EAEC, 11 EHEC, 9 EPEC and 2 ...to kit regents, 3 different groups were ...microbiological evaluation of specimens that ... See full document
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Multicenter Clinical Evaluation of a Novel Multiplex Real Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in Mycoplasma genitalium
... simultaneous detection of ...the detection of ...mutation detection sensitivity of ...(beta) kit is a simple and reliable method for simultaneous detection of ... See full document
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Comparative Assessment of a Commercial Kit and Two Laboratory Developed PCR Assays for Molecular Diagnosis of Congenital Toxoplasmosis
... namely, PCR, and has considerably im- proved the prognosis and outcome for infected children wherever it has been implemented, in particular by eliminating the need for cordocentesis (reviewed in reference ... See full document
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Novel Multiplex Real Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains
... real-time PCR kits for the diagnosis of TB generally identify the MTC but not individual ...(31). Comparative genomics revealed that the M. tuberculosis and ...in M. bovis BCG but which ... See full document
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Evaluation of custom multiplex real time RT PCR in comparison to fast track diagnostics respiratory 21 pathogens kit for detection of multiple respiratory viruses
... real-time PCR (Custom assay) Initial standardisations were done for uniplex real-time PCR using the FTD positive samples as ...and detection of viral nu- cleic ... See full document
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... one-step, multiplex real- time RT-PCR for the detection, quantitation, and differentiation of DENV-1 to -4 and CHIK in patient ...species-specific detection but cannot be used for serotype ... See full document
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Detection and Identification of Mycobacterium tuberculosis in Joint Biopsy Specimens by rpoB PCR Cloning and Sequencing
... 3 M sodium acetate and 250 l of ice-cold ethanol were added, and the mixture was kept at ⫺ 20°C for 10 ...for PCR. rpoB PCR was carried out as described previously ...TA-cloned M. ... See full document
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Multiplex Detection of Three Select Agents Directly from Blood by Use of the GeneXpert System
... T he public health threat posed by the accidental or deliberate release of bacterial select agents remains a serious concern. Francisella tularensis, Bacillus anthracis, and Yersinia pestis are among the top biothreat ... See full document
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Comparison of Anyplex II RV16 with the xTAG Respiratory Viral Panel and Seeplex RV15 for Detection of Respiratory Viruses
... plex PCR and sequencing. The primers for monoplex PCR in the single or nested PCR format were identical with the primers of the RV15 or RV16 ...The PCR products were purified with a power gel ... See full document
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Toxoplasma gondii in livestock in St Kitts and Nevis, West Indies
... Toxoplasma gondii RH strain tachyzoites were grown in pre-cultured Vero cells (ATCC® CCL-81™) in IMDM (supplemented with 2% FCS, 200 IU penicillin/ml and 200 μg/ml streptomycin) at a ratio of 1:2 (Vero cell: T. gondii ... See full document
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5′ Nuclease PCR Assay To DetectYersinia pestis
... Our TaqMan 59 nuclease PCR assay for detection of Y. pestis exhibited satisfactory levels of sensitivity (in the picogram range for both purified bacterial genomic DNA and cloned target sequences) and ... See full document
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