[PDF] Top 20 Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
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Comparison of a loop mediated isothermal amplification for orf virus withquantitative real time PCR
... Orf is distributed throughout many countries [2,17-23], and ORFV infections can cause weight loss and poor de- velopment as the disease prevents host animals from feeding. Therefore, the development of a rapid, ... See full document
8
Comparison of Loop Mediated Isothermal Amplification and Real Time PCR Assays for Detection of Strongyloides Larvae in Different Specimen Matrices
... In a previous study, the Strongyloides LAMP assay was able to detect ⬍ 10 copies of DNA when a plasmid containing the target sequence was serially diluted in Tris-EDTA (18). This indicated that the reaction had a high ... See full document
9
Comparison of Conventional PCR, Multiplex PCR, and Loop Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species
... conventional PCR, mul- tiplex PCR, and enterobacterial repetitive intergenic consensus (ERIC)-PCR were used in previous studies for the rapid detection of Arcobacter ...multiplex PCR described ... See full document
7
Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... The diagnosis of canine neosporosis is based on com- bining appropriate clinical signs and serology such as immunofluorescent antibody tests and enzyme-linked immunosorbent assays (ELISAs) [6]. However, these assays may ... See full document
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<p>Loop-mediated isothermal amplification for detection of <em>Legionella pneumophila</em> in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran</p>
... This study had some limitations. The actual prevalence of Legionnaires’ disease cases may be higher than we reported, as we were investigating only L. pneumophila using PCR and LAMP due to its high environmental ... See full document
6
Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... In comparison with the traditional technique based on virus isolation in embryonated chicken eggs, LAMP can directly detect NDV from samples and would be relatively inexpensive and ...efficient. ... See full document
5
Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription–Loop Mediated Isothermal Amplification
... influenza virus infection recently emerged in China whose clinical features include high mortality and which has also resulted in serious economic ...(H7N9) virus which was the causative agent of this ... See full document
5
Rapid Extraction Method of Mycobacterium ulcerans DNA from Clinical Samples of Suspected Buruli Ulcer Patients
... Abstract: Isothermal amplification techniques such as recombinase polymerase amplification (RPA) and loop-mediated isothermal amplification (LAMP) for diagnosing Buruli ... See full document
11
A Designed Enclosure Unit for Loop-Mediated Isothermal Amplification
... However, due to low thermal conductivity of paraffin wax, it requires large surface area for heat exchange [9]. Kandasamy et al. [10] studied about using the paraffin wax as the PCM-based heat sink. The results from ... See full document
7
Loop mediated isothermal amplification for the detection of goose circovirus
... of PCR in detection of circoviruses in- cluding porcine circovirus type 2 [5] and chicken anemia virus (CAV) ...the virus in 30 min. In comparison the previously described LAMP techniques ... See full document
11
Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... nested PCR, nucleic acid sequence-based amplification (NASBA), and real-time PCR are therefore assumed to be significant for the rapid diagnosis and identification of dengue ... See full document
9
Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... genome amplification (8, ...as real-time quantitative PCR (RT- PCR) ...acid amplification is loop-mediated isothermal amplification (LAMP), developed ... See full document
7
Detection of nucleic acid of classical swine fever virus by reverse transcription loop mediated isothermal amplification (RT LAMP)
... higher amplification efficiency of the RT-LAMP reaction yields a large amount of a by-product, pyrophosphate ion, leading to white precipitate of magnesium pyro- phosphate in the reaction ...a ... See full document
6
Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus
... BK virus (BKV) can cause renal allograft nephropathy (BKVAN), which is now recognized as one of the major man- ifestations of the various syndromes associated with polyoma- viruses ...quantitative ... See full document
7
Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method developed by Eiken Chem- ical ...replace PCR because of its simplicity, rapidity, ... See full document
7
Rapid and Real Time Detection of Chikungunya Virus by Reverse Transcription Loop Mediated Isothermal Amplification Assay
... Clinical samples. A total of 45 acute-phase serum samples collected from patients with a clinical diagnosis of Chikungunya fever who were admitted to Nizam’s Institute of Medical Sciences, Hyderabad, AP, District ... See full document
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A one step reverse transcription loop mediated isothermal amplification for detection and discrimination of infectious bursal disease virus
... transcription loop-mediated isothermal amplification (RT-LAMP) to detect IBDV in one simple step and further identified the very virulent strain from non-vvIBDVs with a simply post- ... See full document
7
Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of West Nile Virus
... WN virus, as shown in Table 1, was established by checking the reactivity with another strain of the WN virus (Eg101), as well as the cross-reactivity with other serologically related members of the ... See full document
7
Rapid detection of the common avian leukosis virus subgroups by real time loop mediated isothermal amplification
... as PCR assays, were developed for the detection of ALV ...promising, PCR requires skilled personnel and specialized high-cost instruments to observe the test results ...[7]. Virus isolation is the ... See full document
8
Host Range and Vertical Transmission of Cyprinid herpesvirus 2
... conducted loop-mediated isothermal amplification (LAMP) assay, PCR and real-time PCR to detect CyHV-2 in the eggs of diseased Carassius auratus, and specific ... See full document
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