[PDF] Top 20 Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
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Comparison of Sequencing by Hybridization and Cycle Sequencing for Genotyping of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... hybridization sequencing may be reliable when used to deter- mine eukaryotic gene sequences, its use with HIV-1 is more complicated for at least two ...using hybridization se- quencing to ... See full document
7
Worldwide Evaluation of DNA Sequencing Approaches for Identification of Drug Resistance Mutations in the Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... resistance genotyping protocols was based on the use of recombinant RT genes derived from a reference virus, into which mutations had been introduced via site-directed ...direct comparison of the ... See full document
6
Novel Recombinant Virus Assay for Measuring Susceptibility of Human Immunodeficiency Virus Type 1 Group M Subtypes To Clinically Approved Drugs
... suppress human immunodeficiency virus (HIV) replication in pa- tients but selects for drug resistance, requiring subsequent resistance-guided therapeutic ...HIV type 1 (HIV-1) ... See full document
11
Targeting human immunodeficiency virus type 1 reverse transcriptase by intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle.
... The HIV-1 RT enzyme is multifunctional and catalyzes the synthesis of a double-stranded DNA copy of the viral RNA (44) by RNA-dependent DNA polymerase, DNA-dependent DNA polymerase, and RNase H activities (21, ... See full document
13
Amino Acid Mutation N348I in the Connection Subdomain of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Confers Multiclass Resistance to Nucleoside and Nonnucleoside Reverse Transcriptase Inhibitors
... affect both subunits of RT. Figure 3 shows that residue 348 of the p51 subunit is located remotely from the polymerase active site ( ⬃ 60 Å) and from the NNRTI binding pocket ( ⬃ 55 Å). Furthermore, it is not in close ... See full document
10
Human recombinant antibody fragments neutralizing human immunodeficiency virus type 1 reverse transcriptase provide an experimental basis for the structural classification of the DNA polymerase family.
... characterization. Sequencing of the DNA coding for the antibody fragments showed that both belong to the VH3 family of human heavy-chain-variable regions (36) while having different ... See full document
7
The K65R Mutation in Human Immunodeficiency Virus Type 1 Reverse Transcriptase Exhibits Bidirectional Phenotypic Antagonism with Thymidine Analog Mutations
... acids 1 to 560) of RT by RT-PCR, purifying the PCR product (Wizard PCR Purification System; Promega, Madison, ...no-drug, virus-infected control ...inhibit virus replication by 50% (IC 50 ) for ... See full document
7
Comparison of Second-Strand Transfer Requirements and RNase H Cleavages Catalyzed by Human Immunodeficiency Virus Type 1 Reverse Transcriptase (RT) and E478Q RT
... FIG. 7. Model of WT RT and E478Q RT polymerase-dependent and polymerase-independent RNase H activities. The possible binding orientations for HIV-1 RT and E478Q RT are shown. (A to C) WT RT and E478Q RT are shown ... See full document
12
The M184V Mutation in Reverse Transcriptase Can Delay Reversion of Attenuated Variants of Simian Immunodeficiency Virus
... simian immunodeficiency virus (SIV) mutants containing deletions within a 97-nucleotide region of the SIVmac239 untranslated region or leader ...in human immunodeficiency virus ... See full document
5
The Impact of Multidideoxynucleoside Resistance-Conferring Mutations in Human Immunodeficiency Virus Type 1 Reverse Transcriptase on Polymerase Fidelity and Error Specificity
... The plates were incubated at 37°C for approximately 15 h before scoring for mutant plaques that did not display the bright blue color of the wild-type M13mp2. These included both clear plaques and plaques with ... See full document
6
Reduced Susceptibility of Human Immunodeficiency Virus Type 1 (HIV-1) from Patients with Primary HIV Infection to Nonnucleoside Reverse Transcriptase Inhibitors Is Associated with Variation at Novel Amino Acid Sites
... Patient virus drug susceptibility was measured by the PhenoSense HIV assay ...cells; virus was harvested and used to infect fresh 293 cells in the presence and absence of ...single cycle assay was ... See full document
5
Incorporation of Human Immunodeficiency Virus Type 1 Reverse Transcriptase into Virus-Like Particles
... VLPs produced by Gag mutants lacking the MA and/or p6 domains were deficient in R560, suggesting that both domains are required for incorporating RT into VLPs. This finding is supported by the Kleiman group report that ... See full document
11
Human immunodeficiency virus type 1 nucleocapsid protein reduces reverse transcriptase pausing at a secondary structure near the murine leukemia virus polypurine tract.
... universal sequencing primer, 5 9 -GTTTTCCCAGTCAC GAC ...The reverse primers were as ...the reverse primers and also provided the ends for subsequent ... See full document
11
Effects of Amino Acid Substitutions at Position 115 on the Fidelity of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
... replication cycle); however, the relative contributions of the various steps and enzymes to this overall error rate are not known (see reference 23 for a ...the virus is actively ... See full document
7
Involvement of Novel Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutations in the Regulation of Resistance to Nucleoside Inhibitors
... HIV-1 genotyping system; Ab- bott Laboratories) (6, ...leukemia virus RT, and amplified with Amplitaq-Gold polymerase enzyme by using two different sequence-specific primers for 40 ...the ... See full document
13
Frequency of Mutations Conferring Resistance to Nucleoside Reverse Transcriptase Inhibitors in Human Immunodeficiency Virus Type 1 Infected Patients in Korea
... direct sequencing methods were used to define human immunodeficiency virus type 1(HIV-1) reverse transcriptase codons 41 to 219 in DNA from 127 peripheral ... See full document
7
Sources and Magnitude of Intralaboratory Variability in a Sequence Based Genotypic Assay for Human Immunodeficiency Virus Type 1 Drug Resistance
... Automated sequencing. Direct population-based dideoxynucleotide cycle se- quencing was performed on unpurified second-round PCR products (A1 and B1) and also in duplicate on samples A2 and B2 as indicated ... See full document
8
An in vivo mutation from leucine to tryptophan at position 210 in human immunodeficiency virus type 1 reverse transcriptase contributes to high-level resistance to 3'-azido-3'-deoxythymidine.
... the reverse transcriptase (RT) region of 26 human immunodeficiency virus type 1 (HIV-1) isolates from eight patients treated with 3 * -azido-3 * -deoxythymidine ... See full document
9
High Degree of Interlaboratory Reproducibility of Human Immunodeficiency Virus Type 1 Protease and Reverse Transcriptase Sequencing of Plasma Samples from Heavily Treated Patients
... dideoxynucleotide sequencing of cultured cell pellets ...involved sequencing of isolates from mixtures of plasmid clones and spiked plasma samples (22; ...different sequencing technologies (9, 11, ... See full document
8
Intracellular expression of single-chain variable fragments to inhibit early stages of the viral life cycle by targeting human immunodeficiency virus type 1 integrase.
... the virus-encoded enzyme integrase ...life cycle, it is an attractive target for therapeutic ...deficiency virus type 1 (anti-HIV-1) IN hybridomas has been used in the ... See full document
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