[PDF] Top 20 Control the Host Cell Cycle: Viral Regulation of the Anaphase-Promoting Complex

... diverse viral families have now been reported to modulate the function of the APC, and more are likely to be discovered, highlighting the potential importance of this cell cycle master regulator in ... See full document

... “wait anaphase” signal that is generated by the spindle assembly checkpoint and is tightly linked with tension sensing mechanisms that communicate the status of chromosome/microtubule ...“wait anaphase” ... See full document

... and complex structure of APC/C have been a challenge for a detailed understanding of this multi-subunit E3 enzyme till ...this complex recognizes and modifies a large number of substrates in a temporally ... See full document

... characterize the genetic interactions observed between these three mutants, we constructed triple mutants and assessed growth rates at various temperatures (Fig. 3C). The triple mutant phenotype was similar to that of ... See full document

... tivation domain-Spo12 fusion as bait (data not shown). indirectly through its possible action on the Cdc14 phos- phatase. A role for Spo12 in the normal regulation of This construct was able to carry out the ... See full document

... the cell undergoes major morphological ...of anaphase and sister chromatids move to opposite spindle ...by cell membrane ingression in a process called cytokinesis (Morgan, ...temporal control ... See full document

... division cycle stages rather than be com- pletely destroyed at the exit of mitosis, as is common in most eukaryotic systems ...negative cell cycle regulation may be favored over protein ... See full document

... The anaphase promoting complex or cyclosome (APC/C) is a multi-subunit cullin-RING E3 ubiquitin ligase that functions to regulate progression through the mitotic phase of the cell cycle ... See full document

... the regulation of mitosis by targeting cell cycle regulators, such as cyclins and cyclin-depen- dent kinases, for ubiquitination (S chwab et ...the regulation of glia ...glial cell ... See full document

... important cell cycle ...kDa complex. It is quite possible that further regulation of this complex is yet to be discovered, and that a number of critical protein-protein interaction ... See full document

... receptor complex, triggering signaling cascades, which converge on the key signaling component RhoA (Fournier et ...in cell cycle regulation, we reasoned that Cdh1-APC-mediated intrinsic axon ... See full document

... The APC is a multi-subunit E3 ubiquitin ligase that is critical for transit through the cell cycle. Although the core subunits are constitutively expressed [29–31], APC functionality oscillates, exhibiting ... See full document

... DISCUSSION We show here for the first time that Apoptin is a nucleocy- toplasmic shuttling protein with a modular structure organized into overlapping functional domains. Whereas previous studies have implicated the ... See full document

... by Tax. In essence, PP2A inhibition by IKKγ-bound Tax maintains IKK in an active, phosphorylated state, causing constitutive phosphorylation and degradation of I-κB, which, in turn, leads to potent activation of genes ... See full document

... the cell cycle can progress in the absence of ...elicit cell death, cancer cells in conjunction with p53 mutation could override the mitotic checkpoint and the cell lethality elicited by ... See full document

... the anaphase-pro- et ...moting complex protein Cdc27/Apc3 concentrates on kineto- stabilize mitotic ...J. Cell Biol. 146: 415–426. chores and chromosome arms in mitosis. Cell Cycle 1: ... See full document

... the cell cycle at ...for cell pellets and 1 ml was harvested for flow cytometry. Cell pellet aliquots were washed with 1 ml ice-cold ddH 2 O and immediately frozen at ⫺80⬚ until extracts were ... See full document

... Address: Departments of Physiology and Biochemistry and Biophysics, University of California, San Francisco, CA 94158, USA. Correspondence: David O Morgan. Email: [email protected] Figure 1 The major biological ... See full document

... Figure 1.5: Phosphorylated Cdh1 is inhibited by complex formation. A, N-terminally tagged FLAG-Cdh1 was purified from acm1∆ cells and incubated with APC via Apc1-TAP immobilized on calmodulin beads. After ... See full document

... intensity projection of 11 z-stacks spaced 4 μm apart (for a total volume of 40 μm). Time is shown in minutes and seconds. Scale bar = 40 μm. See also Figure 1F-G. Movie S3 – CD4 + T cells in 3D culture form small ... See full document