[PDF] Top 20 Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
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Detection of Dengue Viral RNA Using a Nucleic Acid Sequence Based Amplification Assay
... acute dengue infections were received from existing collections in Indonesia, Peru, and ...of dengue infections prior to antibody development, with the exception of four samples that had very low levels of ... See full document
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Real Time Nucleic Acid Sequence Based Amplification Assay for Rapid Detection and Quantification of agr Functionality in Clinical Staphylococcus aureus Isolates
... evaluated using online BLAST searches ...transcription RNA standards (described be- ...investigated using the Autodimer online tool (20). The mfold-RNA and -DNA programs were used to predict ... See full document
5
Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... single-stranded RNA (ssRNA products), which can be de- tected through molecular beacon technology ...DNA detection and quantification system have been described (40), although the molecular mechanism of ... See full document
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Analytical Performance and Clinical Utility of a Nucleic Acid Sequence Based Amplification Assay for Detection of Cytomegalovirus Infection
... for detection of CMV with the NucliSens CMV pp67 ...CMV detection are based on detec- tion either of antigen present in the sample with IFA or of infectious virions with cell culture ...pp67 ... See full document
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Development of a Nucleic Acid Sequence Based Amplification Assay That Uses gag Based Molecular Beacons To Distinguish between Human Immunodeficiency Virus Type 1 Subtype C and C′ Infections in Ethiopia
... the detection of HIV-1-derived RNAs and for genotype ...of RNA eluate obtained from the silica-based isolation method was added to the isothermal amplification reaction mixture, and the ... See full document
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Nucleic Acid Sequence Based Amplification with Oligochromatography for Detection of Trypanosoma brucei in Clinical Samples
... internal-control RNA amplicon (5⬘-CGCTTAGGTCCACTAAG GTACCC-3 ⬘ ), ...respectively. Amplification was detected with an internal probe (5⬘-CAGGTCTGTGATGCTCCTCAATG-3⬘), which is complementary to a se- quence ... See full document
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Colorimetric Nucleic Acid Testing Assay for RNA Virus Detection Based on Circle to Circle Amplification of Padlock Probes
... non-PCR-based nucleic acid detec- tion assay applying padlock probe and C2CA for the detection of ...isothermal amplification strategy and simple colorimetric readout together ... See full document
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Improved Detection of Rhinoviruses by Nucleic Acid Sequence Based Amplification after Nucleotide Sequence Determination of the 5′ Noncoding Regions of Additional Rhinovirus Strains
... Based on these results, new oligonucleotide primers and probes specific for group A and group B viruses were synthe- sized. In comparison with the primers used initially (pd 904, pd 905, pd 906, and pd 907) only ... See full document
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Rapid Detection and Differentiation of Dengue Virus Serotypes by a Real Time Reverse Transcription Loop Mediated Isothermal Amplification Assay
... RT-LAMP assay reported in this study is advantageous due to its simple operation, rapid reaction, and easy ...RT-LAMP assay technique for the rapid detection and differ- entiation of dengue ... See full document
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Nucleic Acid Sequence Based Amplification, a New Method for Analysis of Spliced and Unspliced Epstein Barr Virus Latent Transcripts, and Its Comparison with Reverse Transcriptase PCR
... Nucleic acid sequence-based amplification (NASBA) assays were developed for direct detection of Epstein- Barr virus (EBV) transcripts encoding EBV nuclear antigen 1 (EBNA1), ... See full document
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Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp
... T7 RNA polymerase promoter ...of RNA signal (by T7 RNA polymerase), which may itself be amplified if ...(b) Detection of RNA signal by ELOSA (Enzyme Linked Oli- goSorbant ...The ... See full document
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Nucleic Acid Amplification Assays for Detection of La Crosse Virus RNA
... published sequence of the LAC virus strain Human/78 (GenBank accession number ...virus sequence; primer pairs and probes that demonstrated maximum homology to all LAC virus strains’ M seg- ment polyprotein ... See full document
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Value of Different Assays for Detection of Human Cytomegalovirus (HCMV) in Predicting the Development of HCMV Disease in Human Immunodeficiency Virus Infected Patients
... present viral DNA or incom- plete viral genomes may be amplified (25, ...The detection of HCMV late mRNA in peripheral blood leukocytes may im- prove the ability to identify patients at risk for the ... See full document
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Diagnosis of Human Metapneumovirus Infection in Immunosuppressed Lung Transplant Recipients and Children Evaluated for Pertussis
... the detection of this virus in clinical ...(RT-PCR) assay designed to detect the four genetic lineages of hMPV. This assay and a commercial real-time nucleic acid ... See full document
5
An Update on the Transmission, Pathogenesis, Diagnosis, Treatment and Prevention of Zika Virus Infection
... diagnosed based upon clinical symptoms, the prevalence of vector in the region, and by serological and molecular detection ...patients using animal inoculation and cell lines 52,53 ...the ... See full document
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Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... From the 51 specimens from 33 M pneumoniae PCR-positive patients, 40 were PCR positive upon reextraction-amplifica- tion. Within this group 15 specimens were negative in multi- plex as well as in mono NASBA reactions, ... See full document
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Detection of Mycoplasma pneumoniae in Spiked Clinical Samples by Nucleic Acid Sequence Based Amplification
... 25°C. Nucleic acids were extracted, and NASBA was performed. Nucleic acid ...extraction. Nucleic acids were extracted as described by Boom et ...The nucleic acid-silica complex ... See full document
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Prevalence of Plasmodium spp in malaria asymptomatic African migrants assessed by nucleic acid sequence based amplification
... assays based on Plasmodium antigen detection, but some of these tests perform poorly in cases of low parasitemia [7,9,10] or, in contrast, lead to a false positive diagnosis of malaria due to the ... See full document
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RNA amplification by nucleic acid sequence based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples
... detection in several laboratories (21, 26, 30). In addition, the internal NASBA standard will prevent false-negative results. This indicates that NASBA is a sensitive and reliable amplifi- cation system. Moreover, ... See full document
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Evaluation of the NucliSens Basic Kit for Detection ofChlamydia trachomatis and Neisseria gonorrhoeaein Genital Tract Specimens Using Nucleic Acid Sequence Based Amplification of 16S rRNA
... a detection limit of 1 IFU of ...omp1 assay and 10-fold more sensitive than PCR ...(14). Based on results obtained with 216 genital swab specimens, the Basic Kit had sensitivities of ... See full document
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