[PDF] Top 20 Detection and identification of aquatic birnaviruses by PCR assay
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Detection and identification of aquatic birnaviruses by PCR assay
... the PCR assay with primer set PrB was effective in identifying all of the viruses tested in infected cell cultures (Table 1), this primer set was not effective in detecting virus in the clinical fish ... See full document
5
Quantitative Multiprobe PCR Assay for Simultaneous Detection and Identification to Species Level of Bacterial Pathogens
... Simultaneous detection and/or species identification of mi- croorganisms in a given sample has been reported recently with the multiplexing technique, with multiple sets of species-spe- cific primer pairs ... See full document
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PCR assay based on DNA coding for 16S rRNA for detection and identification of mycobacteria in clinical samples
... A PCR and a reverse cross blot hybridization assay were developed for the detection and identification of mycobacteria in clinical ...The PCR amplifies a part of the DNA coding for 16S ... See full document
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Novel Multiplex PCR Assay for Simultaneous Identification of Community Associated Methicillin Resistant Staphylococcus aureus Strains USA300 and USA400 and Detection of mecA and Panton Valentine Leukocidin Genes, with Discrimination of Staphylococcus aureus from Coagulase Negative Staphylococci
... M-PCR assay for typing MRSA isolates and distinguishing USA300 and USA400 ...The assay specifically involved targeting the Staphylococcus genus-spe- cific 16S rRNA gene sequence (serving to ... See full document
5
Detection and Identification of Leishmania Species from Clinical Specimens by Using a Real Time PCR Assay and Sequencing of the Cytochrome b Gene
... 32). Identification of the species can also help predict the risk of dissemination in immunocompromised patients (10, ...species identification is important to determine the clinical prog- nosis and to ... See full document
6
Analytical Verification of a PCR Assay for Identification of Bordetella avium
... present, identification of this bacterium relies on isolation and biochemical ...a PCR for the detection of ...of PCR conditions for use with the previously described primer pair and ... See full document
7
Detection of Pulmonary Tuberculosis by PCR Assay
... direct detection of Mycobacterium tuberculosis in a significantly shorter time than the two conventional methods mentioned ...commercial PCR systems, different genetic elements such as IS6110, the 38 (Pab ... See full document
7
Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples
... tandem PCR (MT-PCR) assay for the detection and identification of 4 common pathogenic protozoan parasites, Cryptospo- ridium ...MT-PCR assay was compared to four real-time ... See full document
6
Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... real-time PCR assay in a pedi- atric population with severe ARTI clearly illustrated its clinical ...our assay identified a viral pathogen (influ- enza virus or HRSV) in two-thirds of the ... See full document
7
Comprehensive PCR Based Assay for Detection and Species Identification of Human Herpesviruses
... these PCR assays with clinical samples in our institution illustrated that, with the exception of HHV-8, each virus could be detected repeatedly and ...a PCR mistake, the presence of a minority of HSV-1 ... See full document
6
Development of a PCR Assay for Rapid Detection of Enterococci
... Besides identification of bacterial species by species-specific PCR-based assays, identification may also be performed by coupling the amplification of a highly conserved gene with hybridization to ... See full document
7
Nested PCR Assay for Detection of Granulocytic Ehrlichiae
... 5). PCR assays have been used often, mostly in research-oriented settings, and have the advantage of being able to yield positive results during the early acute phase of ...illness. PCR-based assays have ... See full document
6
Multiplex Real Time PCR Assay for Simultaneous Detection of Acanthamoeba spp , Balamuthia mandrillaris, and Naegleria fowleri
... specific identification of these free-living amebas in clinical samples is of crucial importance for efficient case ...TaqMan PCR assay that can simultaneously identify Acanthamoeba ...same ... See full document
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Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR Enzyme Hybridization Assay
... Adenoplex assay has advantages over several of the PCR-based assays recently described in the ...multiplex assay they had poor amplification of sero- types 11, 21, 34, and 35 of species B, yet their ... See full document
5
Identification and detection of Trypanosoma cruzi by using a DNA amplification fingerprint obtained from the ribosomal intergenic spacer
... Identification and Detection of Trypanosoma cruzi by Using a DNA Amplification Fingerprint Obtained from the Ribosomal Intergenic Spacer.. We designed a PCR assay targeted on repeated el[r] ... See full document
6
Rapid Identification of Human Adenovirus Types 3 and 7 from Respiratory Specimens via Multiplex Type Specific PCR
... Moreover, identification of the serotypes known to cause serious disease can be helpful in therapeutic ...multiplex PCR assay was developed for the rapid detection of adenovirus type 3 (Ad3) ... See full document
6
Evaluation of a Novel PCR Based Assay for Detection and Identification of Chlamydia trachomatis Serovars in Cervical Specimens
... In addition to detecting the C. trachomatis plasmid, the Ct-DT assay has been designed to determine the C. trachoma- tis serogroup and serovar. Although the prevalence of C. tra- chomatis serovars described in the ... See full document
6
Perirectal Swab Surveillance for Clostridium difficile by Use of Selective Broth Preamplification and Real Time PCR Detection of tcdB
... 37°C. Identification of ...Cd assay (Gen-Probe Prodesse, Wauke- sha, WI) (described ...by PCR were confirmed as nontoxigenic using a commercial cell culture cytotoxicity assay according to the ... See full document
6
Rapid Identification of Campylobacterspp by Melting Peak Analysis of Biprobes in Real Time PCR
... to PCR-ELISA, the risk of con- tamination is reduced by performing the second-round PCR and product identification in a closed-tube ...The assay is flexible and can be tailored by ... See full document
6
Specific Detection and Identification of Herpes B Virus by a PCR Microplate Hybridization Assay
... HB2B, and HB3B2) were designed in the present study (Fig. 1). HB2B is located on a noncoding intergenic region between the US5 and US6 genes, whereas HB3B2 is located on part of the open reading frame of the US6 gene, ... See full document
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