[PDF] Top 20 Detection of First and Second Line Drug Resistance in Mycobacterium tuberculosis Clinical Isolates by Pyrosequencing

... the pyrosequencing assay. Nevertheless, alternative drug resistance mechanisms cannot be ruled ...ceptible isolates were identified as resistant by the pyrosequencing assay (rrs 1401 ... See full document

... M. tuberculosis complex-specific control (TUB), gyrA amplification control, rrs amplification control, and embB amplifi- cation ...the detection of FLQ resistance, probes cover gyrA; for the ... See full document

... Objectives: Tuberculosis (TB) is the leading cause of death from infectious diseases in the world, with an estimated ...determine drug resistance profiles of Mycobacterium tuberculosis ... See full document

... primary drug resistance indicator among MTB ...fast detection of drug resistance toward first- and second-line anti-TB drugs simultaneously and eliminates the ... See full document

... of Tuberculosis Research once in every 108 ...of drug resistance is a man-made amplification of a naturally occurring ...for tuberculosis predis- poses to the selection of multi-drug ... See full document

... 31 isolates identified as phenotypically XDR, and the GenoType MTBDRsl VER ...FLQ resistance but missed SLID ...and resistance to any one SLID was accepted as a criterion to classify a strain as ... See full document

... the detection of anti-TB drug resistance-associated mutations, including the line probe assays GenoType MTBDR plus (Hain Lifescience GmbH, Nehren, Germany), GenoType MTBDRsl (Hain Lifescience ... See full document

... three isolates that failed to return results by phenotypic DST due to failed regrowth or contam- ...M. tuberculosis DNA, shown by katG sequencing, in the original culture ex- tract of isolate RE180 suggests ... See full document

... RIF resistance, whereas HRMA can also detect INH, fluoroquinolone, and streptomycin resistance in ...M. tuberculosis (35). Moreover, HRMA for the detec- tion of RIF resistance uses no labeled ... See full document

... in drug-resistant clinical isolates of MDR TB is nontrivial; the often incomplete treatment history of subjects and the inaccuracy of phenotypic DST for second-line agents lead to the ... See full document

... the resistance to FQs and second-line injectable drugs (KAN and CAP) in ...rifampin/isoniazid resistance [16], which has been used in Russia for more than 5 ...FQ resistance and ... See full document

... without drug and incubated at ...of drug that inhibited more than 99% of the bacterial ...each drug, the bac- terial growth was measured and the percentage of resistance was calculated, ... See full document

... The mycobacterium growth indicator tube (MGIT960) automated liquid medium testing method is becoming the international gold standard for second-line drug susceptibility testing of multidrug- ... See full document

... rapid detection of XDR ...with drug resistance detec- ...to drug resistance detection made by Guo et ...test clinical specimens, which significantly shortens the turn- ... See full document

... cin-resistant isolates were available for testing in all studies (n ⫽ 5, n ⫽ 8, and n ⫽ 13), and testing of a larger number of aminoglycoside-resistant isolates is required to define sensitiv- ity ... See full document

... TB drug-resistant within a human popula- tion. We first propose a drug resistance in a tuberculosis transmission model with two strains of Mycobacterium tuberculosis: ... See full document

... Tuberculosis (TB) is one of the most widespread infectious diseases in the world. In 2016, 0.4 million People fell ill with TB, and 1.7 million died from this disease globally, with over 95 % of the TB deaths ... See full document

... M. tuberculosis isolates by the BACT/MGIT 960 system from patients with osteoarticular tuberculosis was collected for further ...The detection of rpoB gene mutations was performed using ... See full document

... (v) Pyrosequencing. Pyrosequencing was performed on the 102 clinical isolates and on the ATCC reference strain according to the instructions of the manu- facturer (Amersham Biosciences, ... See full document

... Probe and primer design. Six dually labeled probes and 10 primers were designed to detect mutations in five drug resistance-associated genes (Table 1). For the 81-bp rpoB core region, multiple probes ... See full document