[PDF] Top 20 Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
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Development of Animal Models for Adeno-Associated Virus Site-Specific Integration
... Rep-mediated integration occurs fol- lowing AAV injection into muscle cells, although the efficiency was ...of integration. Furthermore, targeted integration was not detected in all of the injected ... See full document
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Kinetics and Frequency of Adeno-Associated Virus Site-Specific Integration into Human Chromosome 19 Monitored by Quantitative Real-Time PCR
... AAV site-specific ...highly specific for AAV ITR/AAVS1 ...AAV-2 integration into chr-19 ...the integration efficiencies of AAV ITR and/or rep ...for site specificity of ... See full document
6
Reduction of MBS85 gene expression after the targeted integration of a transgene into the AAVS1 site using adeno-associated virus integration machinery
... Sitespecific integration in KM102 cells: We analyzed 30 KM102 clones by Southern blotting after digestion with EcoRV. Figure 3A shows a representative blot. The left panel is a blot that was hybridized with the ... See full document
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Adeno-Associated Virus Type 2 p5 Promoter: a Rep-Regulated DNA Switch Element Functioning in Transcription, Replication, and Site-Specific Integration
... a specific type of Rep-responsive Pol II transcription initiation ...helper virus, we could not detect replication with any of the constructs including ...helper virus, we could not detect p5 ... See full document
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Roles of Adeno-Associated Virus Rep Protein and Human Chromosome 19 in Site-Specific Recombination
... integrates site specifically into primate ...for site-specific recombination after infection with transducing vectors expressing ...for site-specific recombination are contained within ... See full document
14
Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... simian virus 40 promoter-driven neomycin (neo)-resistance protein expression cassette derived from plasmid pHyRGN (10) into pHSVloxP ...BglII site, and the 5⬘ AAV ITR into the NotI and PacI sites of ... See full document
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Adeno-associated virus vector integration junctions.
... from adeno-associated virus (AAV) have the potential to stably transduce mammalian cells by integrating into host ...the integration of wild-type AAV have identified a common ... See full document
8
Conditional Site-Specific Integration into Human Chromosome 19 by Using a Ligand-Dependent Chimeric Adeno-Associated Virus/Rep Protein
... Rep-mediated site-specific integration at AAVS1 takes place in transgenic rodents (mice and rats) carrying the human AAVS1 site (43), and it would be of interest to test the inte- gration ... See full document
14
Mutational analysis of the adeno-associated virus Rep68 protein: identification of critical residues necessary for site-specific endonuclease activity.
... viral integration into the host genome (11, 25, 44, ...nicking site next to a Rep binding site within the preferred integration locus is required for the targeting of AAV integration ... See full document
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Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1
... Adeno-associated virus (AAV) is a helper-dependent parvovirus that requires coinfection with adenovirus (AdV) or herpes sim- plex virus 1 (HSV-1) to ...helper virus, AAV can persist in ... See full document
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Adeno-Associated Virus Rep Represses the Human Integration Site Promoter by Two Pathways That Are Similar to Those Required for the Regulation of the Viral p5 Promoter
... Western blot analysis. Forty-eight hours after transfection, cells were lysed in RIPA buffer (50 mM Tris-HCl [pH 8], 150 mM NaCl, 0.1% SDS, 1% Nonidet P-40, 0.5% sodium deoxycholate, 1 ⫻ Complete protease inhibitor ... See full document
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High-Throughput Sequencing Reveals Principles of Adeno-Associated Virus Serotype 2 Integration
... The integration of wtAAV places the GAGC sequences of the viral P5 promoter and 5= ITR in the same orientation as the hu- man genomic GAGC sequences when the integration occurs ad- jacent to the RBS but in ... See full document
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A novel terminal resolution-like site in the adeno-associated virus type 2 genome.
... a site-specific and strand-specific endonuclease activity which specifically cleaves at the terminal resolution site (trs) within the ITR sequences (10, 11, ...a specific sequence at ... See full document
7
Identification of an Adeno-Associated Virus Rep Protein Binding Site in the Adenovirus E2a Promoter
... Adeno-associated virus (AAV) is a nonpathogenic human parvovirus that normally depends on a helper virus to effi- ciently complete its replication cycle (reviewed in reference ...replication, ... See full document
11
Site Specific Modification of Adeno-Associated Virus Enables Both Fluorescent Imaging of Viral Particles and Characterization of the Capsid Interactome
... rare integration into the host ...a site-specific tetracysteine sequence into the AAV serotype 9 (AAV9) capsid, to permit labelling of viral particles with either a fluorescent dye or ... See full document
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Identification and Characterization of an Adeno-Associated Virus Integration Site in CV-1 Cells from the African Green Monkey
... the integration rates in Cos-7 and HeLa cells after contransfection of the AAV2 Rep68 and AAV2 ITR constructs pDD-puro and ...for site-specific integration was performed on the stable ... See full document
12
A Helper-Dependent Capsid-Modified Adenovirus Vector Expressing Adeno-Associated Virus Rep78 Mediates Site-Specific Integration of a 27-Kilobase Transgene Cassette
... detailed integration studies in colonies with ⬎ 30% GFP ⫹ ...vector integration. Southern blot analysis using an AAVS1-specific probe revealed no chromosomal DNA rearrangements in clones from ... See full document
11
Transcriptional Analysis of the Adeno-Associated Virus Integration Site
... integrate site specifically into a ubiquitously transcribed region raises several questions with regard to the nonpathogenic character of ...the virus integrate, and thus disrupt a transcriptional unit, in ... See full document
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Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... NotI site (blunt end repaired) of ...SphI site (blunt) of pHSVNot to form ...BamHI site of pUC18-Not, a derivative of pUC18 in which the multiple cloning site is flanked by NotI sites ...NotI ... See full document
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Adeno-Associated Virus Site-Specific Integration Is Mediated by Proteins of the Nonhomologous End-Joining Pathway
... Adeno-associated virus (AAV) is a ubiquitous human virus ( ⬃ 80% of adults are seropositive [4]); like other nuclear DNA viruses, it causes persistent ...helper virus coinfection, and ... See full document
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