[PDF] Top 20 Development of Reverse Transcription PCR Assays Specific for Detection of Equine Encephalitis Viruses
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Development of Reverse Transcription PCR Assays Specific for Detection of Equine Encephalitis Viruses
... IC viruses than the VEE-IE virus strain Mena II (14, ...IC viruses, frequently occurred in South America, including a 1969 to 1972 pandemic which spread from Central America to Texas in the United ... See full document
9
Duplex Reverse Transcription PCR Followed by Nested PCR Assays for Detection and Identification of Brazilian Alphaviruses and Flaviviruses
... eastern equine encephalitis virus (EEEV), western equine encephalitis virus (WEEV), Aura virus (AURAV), Venezuelan equine encephalitis virus (VEEV, subtype I, vari- ety F), ... See full document
7
Development and Validation of Multiplex One Step Real Time TaqManqRT PCR Assays for Detection and Quantification of Arboviral Encephalitis Viruses
... Arboviral encephalitis is caused by infection with an arbovirus, which transmit- ted by a mosquito, tick or another ...Eastern equine Encephalitis virus (EEEV) [4] [5], Western Equine Encepha- ... See full document
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Design and Validation of Real Time Reverse Transcription PCR Assays for Detection of Pandemic (H1N1) 2009 Virus
... RT-PCR assays based on the available se- quences for pandemic (H1N1) 2009 virus recovered at the outset of the ...those assays to the performance characteristics of assays provided by the CDC ... See full document
7
A duplex real time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses
... pathogen detection including indirect hemagglutination inhibition (IHI), ELISA, virus isolation and ...containment. Reverse-transcriptase PCR (RT-PCR) is a rapid and sensitive method that is ... See full document
5
Development of one step quantitative reverse transcription PCR for the rapid detection of flaviviruses
... the reverse primer Flavi all AS2 for YFV, DENV type 2 and Usutu virus did not have any impact as these three viruses were detected well by Pan-Flavi assay (Figure ...for detection of all flaviviruses ... See full document
11
Detection of North American Eastern and Western Equine Encephalitis Viruses by Nucleic Acid Amplification Assays
... TaqMan assays provided con- sistent sensitivities when multiple preparations of EEE and WEE viral dilutions were tested (data not ...amplification assays has been demonstrated in recent studies indicating ... See full document
7
Real time reverse transcription PCR based sequencing independent pathotyping of Eurasian avian influenza A viruses of subtype H7
... influenza viruses (LPAIV) of the subtypes H5 and H7 are known to give rise to highly pathogenic (HP) phenotypes by spontaneous insertional mutations which convert a monobasic trypsin-sensitive endoproteolytical ... See full document
10
Evaluation of Three Influenza A and B Real Time Reverse Transcription PCR Assays and a New 2009 H1N1 Assay for Detection of Influenza Viruses
... influenza. PCR-based methods provide rapid and sensi- tive detection and, most importantly, help with identifying different subtypes of influenza ...RT-PCR assays for detection of all ... See full document
6
Comparison of Flavivirus Universal Primer Pairs and Development of a Rapid, Highly Sensitive Heminested Reverse Transcription PCR Assay for Detection of Flaviviruses Targeted to a Conserved Region of the NS5 Gene Sequences
... three viruses of the Bunyaviridae family, and three viruses of the Togaviridae family were used as negative con- trols, and nonspecific amplifications were not ...DEN viruses, one WN strain, and Zika ... See full document
6
Rapid Detection of Respiratory Tract Viral Infections and Coinfections in Patients with Influenza Like Illnesses by Use of Reverse Transcription PCR DNA Microarray Systems
... available reverse transcription-PCR (RT-PCR) DNA microarray systems allowing rapid detection of influenza A virus strains, including the new A/H1N1v strain as well as 20 other common or ... See full document
7
Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... I detection. The SYBR green detection method was adapted for the C-prM and 3 ⬘ NC amplimer ...gue viruses (8, 14, 19, ...negative PCR results has previously been observed (8, 20; ...-4 ... See full document
10
Development and Evaluation of One Step TaqMan Real Time Reverse Transcription PCR Assays Targeting Nucleoprotein, Matrix, and Hemagglutinin Genes of Equine Influenza Virus
... real-time reverse transcription-PCR (rRT-PCR) assays by the use of the minor groove binding probe to detect a wide range of equine influenza virus (EIV) strains comprising both ... See full document
7
Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single Tube Multiplex Reverse Transcription PCR: Feasibility Study
... as PCR (29) and reverse transcription (RT)-PCR (RT- PCR) are highly sensitive techniques for the detection of nu- cleic acid sequences from viruses and bacteria in ... See full document
7
Multiplex reverse transcription PCR for surveillance of influenza A and B viruses in England and Wales in 1995 and 1996
... Multiplex PCR assays capable of amplifying 13 or more separate regions of chromosomal DNA and of detecting and typing several bacterial and viral pathogens have also been described (3; reviewed in reference ... See full document
7
Serotype Specific Detection of Dengue Viruses in a Fourplex Real Time Reverse Transcriptase PCR Assay
... following viruses were used as prototypes to prepare standard dilutions for each serotype: DEN-1 Hawaii 44, DEN-2 New Guinea C 44, DEN-3 H-87 (Philippines 56), and DEN-4 H-241 (Philippines ...Japanese ... See full document
7
Monitoring Shedding of Five Genotypes of RotaTeq Vaccine Viruses by Genotype Specific Real Time Reverse Transcription PCR Assays
... time reverse transcription-PCR (RT-PCR) ...RT-PCR assays were designed for the VP7 gene in genotypes G1, G2, G3, G4, and ...All assays exhibited excellent linearity, and ... See full document
9
Development of real time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
... for detection of viral nucleo- protein was developed as an alternative method to virus isolation ...rapid detection of PPRV, however, its sensitivity was not good enough to detect PPRV in clinical samples ... See full document
10
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... 2 viruses have been distinguished, three of which (subclades 1, 2, and 3) also differ in geographical distri- bution ...the development and validation of a real-time, one-step reverse ... See full document
9
Reverse transcription, loop mediated isothermal amplification assay for the sensitive and rapid detection of H10 subtype avian influenza viruses
... influenza viruses, NDV, IBV and ILTV) indicating a lack of amplifi- cation ...the detection assay for each strain are shown in Table ...influenza viruses (H1N1, H3N2 and influenza B virus), thus val- ... See full document
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