[PDF] Top 20 Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
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Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... positive dengue-specific IgM and/or IgG antibod- ies as the cell culture ...for real-time RT-PCR was added for each reac- tion, respectively) ...(ii) real-time ... See full document
9
Development and Evaluation of Serotype and Group Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus
... (TaqMan) reverse transcriptase PCR (RT-PCR) assays were developed for serotypes 1 to 4 and group-specific detection of dengue ...virus. Serotype- and ... See full document
6
Development of Real Time Reverse Transcriptase PCR Assays To Detect and Serotype Dengue Viruses
... for assay optimization. The am- plification profile (RT-PCR cycle condition and amplimer/probe concentration) and the amplimer and probe specificities and sensitivities were determined using the viral RNA ... See full document
10
Development of a SYBR green I based RT PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti
... SYBR Green I-based one-step real-time quantitative RT- PCR amplification was performed in a LightCycler Car- ousel-based system (Roche, ...the ... See full document
8
Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... pairs specific for the BVDV NS5B region were designed as 5 ’ -ACACCAAAGCCTGGGA- CACT-3 ’ (position 11226-11245 of the NADL sequence) and 5 ’ -CTCCCTCTCTGCCCATTTCTT-3 ’ (position 11386-11406 of the NADL ...vitro ... See full document
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Development and Validation of a Quantitative, One Step, Multiplex, Real Time Reverse Transcriptase PCR Assay for Detection of Dengue and Chikungunya Viruses
... CHIKV: virus propagation and isolation in susceptible cell lines, reverse transcriptase PCR (RT- PCR), and serology (immunoglobulin M [IgM] and IgG enzyme- linked immunosorbent assays ... See full document
8
Multiplex Real Time Reverse Transcription PCR Assay for Determination of Hepatitis C Virus Genotypes
... genotyping assay was described by Okamoto et al. (24). The assay utilized a first-round amplification of a large section of the core region, followed by a set of second nested amplification reactions each ... See full document
8
Development and application of SYBR Green I real time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B
... the virus by PCR amplification of the specific frag- ment within the genome is usually carried ...is based on the gene sequence of the ALV gp85 ...no specific dissociation curve was de- ... See full document
10
The development of a rapid SYBR one step real time RT PCR for detection of porcine reproductive and respiratory syndrome virus
... rapid, specific and sensitive assays are required for diagnosis of PRRSV in ...of virus in cell cultures is technically diffi- cult and time-consuming and thus is not suitable for rou- tine ... See full document
7
SYBR green based one step quantitative real time polymerase chain reaction assay for the detection of Zika virus in field caught mosquitoes
... of virus RNA by molecular methods such as polymerase chain reaction (PCR) and related technolo- gies; ...probe-based real- time reverse transcription PCR ... See full document
7
Establishment of one step SYBR green based real time PCR assay for rapid detection and quantification of chikungunya virus infection
... plaque assay remains the “gold standard” although it has the disadvantage that longer than 7 days is usually required to complete the ...ELISA- based techniques [9] for the quantification of CHIKV envelope ... See full document
7
A robust HIV-1 viral load detection assay optimized for Indian sub type C specific strains and resource limiting setting
... RNA Real-TM Quant (Sacace Biotechnologies S.r.l, Italy) and HIV-1 Real Time RT PCR kit (Life River, Shanghai ZJ Biotech, China) respectively] shown in this study may be due to presence of ... See full document
8
Potentials and limitations of molecular diagnostic methods in food safety
... the development of a test able to detect and determine the serotype of pathogenic strains [29, 31, ...a specific serotype is particularly important with ...The assay relies on ... See full document
12
Development and Application of a Universal Hemoplasma Screening Assay Based on the SYBR Green PCR Principle
... samples. Specific conventional and quantitative real-time TaqMan PCR systems have been established to diagnose hemotropic mycoplasmas (5, 11, 13, 18, 20, 25, ...conventional PCR is ... See full document
6
Detection and Differentiation of Leishmania spp in Clinical Specimens by Use of a SYBR Green Based Real Time PCR Assay
... ITS1-specific SYBR green PCR (LSG-qPCR) assay, we designed primers LSGITS1-F1 (CATTTTC CGATGATTACAC) and LSGITS1-R1 (CGTTATGTGAGCCGTTATC) on the basis of multiple alignments of the rRNA ITS1 ... See full document
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Rapid Simultaneous Detection of Enterovirus and Parechovirus RNAs in Clinical Samples by One Step Real Time Reverse Transcription PCR Assay
... below assay sensitivity levels due to the limited penetration of virus into the CNS and because sampling often is conducted in the resolution phase of the acute infection, when symptoms may be most ... See full document
5
Multiplex real-time reverse transcription polymerase chain reaction for diff erential detection of H5, N1, and N8 genes of highly pathogenic avian infl uenza viruses
... this, one diag- nostic strategy would be to combine the proposed mRRT-PCR assay and the previously described AIV M-gene-specific RRT-PCR (Kim et ...RRT- PCR needs to be developed ... See full document
10
Detection of Dengue Virus Replication in Peripheral Blood Mononuclear Cells from Dengue Virus Type 2 Infected Patients by a Reverse Transcription Real Time PCR Assay
... Although dengue virus replicates in a variety of cells in vitro, including cells of the myeloid, lymphoid, epithelial, endothe- lial, and fibroblastic lineages, the cell types supporting dengue ... See full document
7
Real Time Reverse Transcription PCR Assay for Detection of Mumps Virus RNA in Clinical Specimens
... different PCR master mix setup, the TaqMan PCR Core reagents kit with Multiscribe reverse transcriptase (data not shown) (Applied ...gene real-time assay. We compared the ... See full document
7
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... the PCR assay than in vitro-transcribed RNA ...is based on RNA and could easily be adapted and integrated into the assay to check for successful RNA extraction and ... See full document
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