[PDF] Top 20 Dynamics of molecular parameters of human immunodeficiency virus type 1 activity in vivo.

... In particular, we studied the time course of three quantitative molecular parameters of viral activity genomic RNA copy number in plasma and provirus and late HIV-1 transcript molecule c[r] ... See full document

... in vivo and during replication of primary HIV-1 in peripheral blood mononuclear cells (PBMC) and to determine the extent of vif sequence variability within the replicating virus ...weeks) ... See full document

... Little information regarding the kinetics of HIV-1 replica- tion in vertically infected infants and children is available. We therefore undertook a study in which potent antiretroviral therapies were used to probe ... See full document

... the dynamics of this preintegration form of latency are not directly relevant to the stable postintegration form of HIV-1 latency that rep- resents a major barrier to eradication of the infection with ... See full document

... simian immunodeficiency virus (SIV), HIV-1, and many drug-resis- tant clinical HIV-1 isolates ex vivo ...the molecular basis for substrate-inhibitor specificities will provide a ... See full document

... leukemia virus reverse transcriptase (Bethesda Research Laboratories), 40 U of RNasin (Boehringer Mannheim), 20 pmol of polNE1 primer, ...triphosphates, 1 3 PCR buffer (50 mM KCl, 10 mM Tris-HCl [pH ...a ... See full document

... evolutionary dynamics of this region thought to be due to strong host-pathogen interac- tions (5, 27, ...the activity of the haplotypes found in different populations, as some of these haplotypes provide ... See full document

... cell-free virus and infected cells is much higher than previ- ously believed (47, 67, ...HIV-1 activity, as reflected by increased mean values of bio- logical and molecular determinants of ... See full document

... of human immunodeficiency virus type 1 (HIV-1) in vivo infection is the rapid generation and turnover of viral variants, resulting in a high degree of sequence diver- sity ... See full document

... In summary, we developed an ECL-based p7 immunoassay with a dynamic range of 50 to 20,000 pg of p7 per ml. It was well suited to compete with p24 assays to monitor standard viral infections, independent of which ... See full document

... of human immunodeficiency virus type 1 (HIV-1)-infected individuals remains an under-evaluated and somewhat enigmatic ...the virus as well as a location of HIV-1 ... See full document

... primary human peripheral blood mononuclear cells less efficiently than viruses containing the ...functional activity of the ADA envelope glycoproteins may account for the observation that, even when ... See full document

... pharmacovirological parameters of INI treat- ment in ...in vivo. Furthermore, our study demon- strates that INI treatment in vivo is accompanied by an eleva- tion of levels of unintegrated cDNA ... See full document

... the human immunodeficiency virus type 1 long terminal repeat (LTR) is activated by ultraviolet ...in vivo, transgenic mice carrying the lacZ gene under the control of the viral ... See full document

... Current assays cannot probe early fusion dynamics at single-molecule resolution in live cells and in real time. Traditional assays have provided an important mechanistic understanding of the fusion process, which ... See full document

... for virus isolation or polymerase chain reaction (PCR) for HIV-1 DNA sequence detection, we and others (2–6) demonstrated that a consistent proportion of infants who were subsequently recog- nized as ... See full document

... of virus replication was 13 μM, and at this dose the TCBs were 80% viable by the Trypan ...in vivo potential because the parent drug amifostine can be administered at very high doses with virtually no ... See full document

... of human immunodeficiency virus type 1 (HIV-1) in primary blood lymphocytes, certain T-cell lines (nonpermissive cells), and most likely in vivo is highly dependent on the ... See full document

... tropic molecular clone of HIV-1 (1) that has been well char- acterized in the SCID-hu mouse model (2, ...(Fig. 1) because, unlike nef deletion mutants of NL4-3, which are attenuated for ... See full document

... in vivo (27, ...the dynamics of episomal viral cDNA turnover in patients on long-term suppressive ther- ...in vivo evolu- tion and turnover of episomal viral ... See full document