[PDF] Top 20 Effect of different donor cells on human immunodeficiency virus type 1 replication and selection in vitro.

... in vitro selection, as well as those influencing viral transmission in vivo, are not fully ...in vitro cocultivation of PBMC releases the virus from immune system selective pressures, such as ... See full document

... T cells, and the rapid-turnover assay was performed every third day ...infected cells were collected and extrachromosomal DNA was isolated by Hirt extraction and used for cloning of the ...mutant ... See full document

... After 1 wk in culture cell supernatants were tested for reverse transcriptase (RT) ...the replication of HIV-1 in both primary cord blood and adult donor MDM in a concentration dependent ... See full document

... the effect of IL-12 and IL-15 on HIV replication were initiated because these cytokines have fea- tures suggesting that they may enhance cellular ...little effect on basal HIV-1 p24 antigen ... See full document

... of human immunodeficiency virus type 1 consists of a noncovalently linked dimer of two identical, unspliced RNA ...the type of interaction is termed loop-loop ...in ... See full document

... tethering donor vesicles to their respective target ...Marburg virus-infected cells, suggesting that Rab9 is utilized in Marburg ...in human immunodeficiency virus (HIV) ... See full document

... in vitro (21, 24, 33, 35, 37–39, 57) and in vivo (2, 16, 26) evidence for this theory; however, other studies have not supported it (12, 19, 31, 32, 47, ...HIV replication in coinfected DC cultures and that ... See full document

... T cells, CXCR4 allows virus entry but does not activate signalling pathways leading to an enhancement of Tat ...the effect of SDF-1 in primary ...increased replication of R5 strains was ... See full document

... of virus produced by different cellular sources and a differential susceptibility of these sources to the effects of antiretroviral therapies could also explain the observed age-related differences in viral ... See full document

... with the use of the mammalian transfection kit (Stratagene, San Diego, CA). The first set of transfected cells was used for the collection of total RNA, while the second set was used for the collection of ... See full document

... 0.3, 1, and 3 ␮g of rIL-16 per ml in MDMs indicated that the highest concentration induced 91 to 100% inhibition (mean ⫾ standard error of the mean [SEM], 95% ⫾ ...the virus-suppressive activity of rIL-16 ... See full document

... 293 cells (ATCC) using Superfect ...HIV-1 virus capsid protein (p24) quantitation was performed on virus stocks using an HIV-1 p24 antigen enzyme-linked immu- nosorbent assay ...PM1 ... See full document

... that human immunodeficiency virus type 1 (HIV-1)-infected human thymocytes, in the absence of any exogenous stimulus but cocultivated with autologous thymic epithelial ... See full document

... NHOK cells infected with ...in vitro with an efficiency approximately one- fourth of that with which it entered an equivalent number of PBLs ...heat-inactivated virus, confirming that the ... See full document

... Human immunodeficiency virus type 1 (HIV-1) entry is an attractive target for therapeutic ...in vitro by serial virus passage in the presence of increasing T1249 ... See full document

... against human immunodeficiency virus ...HIV type 1-infected individuals (13). NAbs are identified in vitro using “conventional” neutralization assays with phytohemag- glutinin ... See full document

... T cells and T-cell lines. There are three different NOS enzymes, one of them (iNOS) being in- ducible by cytokines such as TNF-␣ and other proinflamma- tory stimuli in macrophages and several other cell ... See full document

... T cells as well as the ability to confer cyclosporine resistance to T cells ...support virus production may be a result of their inability to adequately stimulate resting CD4 ⫹ T cells, but it ... See full document

... ⫹ cells have been described to predominantly exhibit a CD45RA ⫺ CD27 ⫹ CD57 ⫺ CCR7 ⫺ phenotype characteristic of transitional memory cells ...⫹ cells or those that are specific for other viruses ...⫹ ... See full document

... uninfected human cells enhances the produc- tion of infectious virions from infected rodent ...rodent cells to produce infectious HIV-1 par- ticles could be due to a lack of essential cellular ... See full document