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Efficiency and selectivity of RNA packaging by Rous sarcoma virus Gag deletion mutants.
... To gain information about which portions of Gag is required for RNA packaging in the avian sarcoma and leukemia virus system, we utilized a series ofgag deletion mutants that retain the [r] ... See full document
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Yeast Three-Hybrid Screening of Rous Sarcoma Virus Mutants with Randomly Mutagenized Minimal Packaging Signals Reveals Regions Important for Gag Interactions
... double-stranded RNA he- lices form a different geometry (A-form versus B-form of the DNA helix), and so they create different surfaces for protein binding; a narrow, deep major groove and a shallow, wide minor ... See full document
8
In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles.
... of RNA, which showed no tendency to aggregate even after several ...these virus-like particles were very similar to those produced by DPR, except for their failure to aggregate and their slightly smaller ... See full document
11
Importance of Basic Residues in Binding of Rous Sarcoma Virus Nucleocapsid to the RNA Packaging Signal
... for RNA binding and efficient ...genomic RNA binding, and hence packaging, we have used the yeast three-hybrid binding ...a Gag molecule containing all of the domains, except ...efficient ... See full document
12
trans-Acting Inhibition of Genomic RNA Dimerization by Rous Sarcoma Virus Matrix Mutants
... MA mutants had the same RNA dimerization defect as Myr1E, virus particles were collected following transfection of QT6 cells with proviral plas- mids and viral RNA was isolated and subjected ... See full document
9
Characterization of Rous Sarcoma Virus Gag Particles Assembled In Vitro
... genomic RNA clearly is largely nonspecific, just as we have found in ...the packaging sequence psi, and recent evidence suggests that the mass of RNA in par- ticles lacking a genome is similar to the ... See full document
12
Evidence for a second function of the MA sequence in the Rous sarcoma virus Gag protein.
... first mutants of this kind described for RSV, and a function other than membrane binding has not been demonstrated pre- viously for the RSV MA ...for RNA incorporation into virions, since replacement of the ... See full document
11
Genetic Evidence for a Connection between Rous Sarcoma Virus Gag Nuclear Trafficking and Genomic RNA Packaging
... each Gag- GFP fusion protein, the average levels of fluorescence in the nucleus (defined as the nuclear fluorescence divided by the total cell fluorescence) in the absence and presence of LMB treatment was ... See full document
8
Autogenous regulation of RNA translation and packaging by Rous sarcoma virus Pr76gag.
... of Gag and ribosomes to RL mRNA and the cellular concentrations of ribosomes and Gag protein, ...the Gag protein concentrations in these cell lines were about the ...the Gag protein ... See full document
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Genetic analysis of the major homology region of the Rous sarcoma virus Gag protein.
... viral RNA, its function in viral replication is largely ...release, mutants of Rous sarcoma virus were created in which segments of CA were deleted or single conserved residues in the ... See full document
15
RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant
... RSV Gag protein is shown at the top with the MA, p2, and p10 cleavage products indicated; the numbers below refer to amino acid ...10 gag codons were replaced with the sequence encoding the myristylated Src ... See full document
9
Hydrodynamic and Membrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein
... which Gag proteins interact with mem- branes are poorly understood. HIV-1 Gag from an in vitro translation system can sense both the hydrophilic and the hy- drophobic parts of the bilayer ...purified ... See full document
12
Characterization of ribosome binding on Rous sarcoma virus RNA in vitro.
... Our results suggest that i translation in vitro of Rous sarcoma virus virion RNA results in the synthesis of at least two polypeptides; ii the pattern of ribosome binding observed for Ro[r] ... See full document
8
Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein
... mutant Gag proteins. De- spite the marked reduction in VLP release, Gag proteins con- taining NES mutations were still capable of budding, suggest- ing either (i) the block to nuclear export is incomplete, ... See full document
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Structural Characterization of the Rous Sarcoma Virus RNA Stability Element
... tion equilibrium data were collected at three rotor speeds (9,000, 10,500, and 12,000 rpm) at 20°C with a loading concentration of 20 g/ml monitored at 260 nm. Data plots were offset by 0.2 absorbance units (AU) for ... See full document
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Cooperative transformation studies with temperature-sensitive mutants of Rous sarcoma virus.
... The present report deals with the methods used for the isolation of ts mutants from stocks of Bryan high-titer RSV of the morphr and morpht type of morphological conversion 14, with the [r] ... See full document
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Transformation-defective Rous sarcoma virus mutants with altered p19 of the gag gene and their inhibitory effect on host cell growth.
... 3 Transformation-Defective Rous Sarcoma Virus Mutants with Altered p19 of the gag Gene and Their Inhibitory Effect on Host Cell Growth Received 22 November 1982/Accepted 17 March 1983 Mu[r] ... See full document
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Transport and processing of the Rous sarcoma virus Gag protein in the endoplasmic reticulum.
... The Gag and Env proteins illustrated here were used to create the chimeras diagrammed in panels B and ...a Gag derivative in which the first 10 amino acids of the Src protein provide a site for the addition ... See full document
10
Unusual features of the leader sequence of Rous sarcoma virus packaging mutant TK15.
... Nucleotide sequence analysis of the provirus DNA of the mutant revealed that the deletion extends from the 3' end of the primer binding site to 22 bases upstream of the gag initiation co[r] ... See full document
5
Rous sarcoma virus nucleic acid-binding protein p12 is necessary for viral 70S RNA dimer formation and packaging.
... 450-459 0022-538X/86/110450-10$02.00/0 Copyright C 1986, American Society for Microbiology Rous Sarcoma Virus Nucleic Acid-Binding Protein p12 Is Necessary for Viral 70S RNA Dimer Format[r] ... See full document
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