[PDF] Top 20 Exploration of Biases That Affect the Interpretation of Restriction Fragment Patterns Produced by Pulsed Field Gel Electrophoresis
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Exploration of Biases That Affect the Interpretation of Restriction Fragment Patterns Produced by Pulsed Field Gel Electrophoresis
... In RFP analyses the subjective process of band determina- tion is critical and cannot be ignored (12, 28, 30). Conse- quently, we incorporated various types of errors into our anal- yses. The divergence from the true ... See full document
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Comparison of a PCR Restriction Fragment Length Polymorphism (PCR RFLP) Assay to Pulsed Field Gel Electrophoresis To Determine the Effect of Repeated Subculture and Prolonged Storage on RFLP Patterns of Shiga Toxin Producing Escherichia coli O157:H7
... toxin produced by STEC has been considered a prime virulence factor ...and pulsed-field gel electrophoresis (PFGE), have been utilized for molecular ep- idemiological studies (10, ... See full document
6
A Novel Multiresistant Streptococcus pneumoniae Serogroup 19 Clone from Washington State Identified by Pulsed Field Gel Electrophoresis and Restriction Fragment Length Patterns
... PFGE patterns was performed by visual inspection of the ...SacII produced PFGE patterns of 10 to 15 DNA bands between ...digests produced PFGE patterns with 8 to 12 DNA bands between ... See full document
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Comparison of restriction enzyme analysis and pulsed field gradient gel electrophoresis as typing systems for Candida albicans
... Distinguishable restriction enzyme patterns of chromosomal DNAs were produced by using endonucleases MspI and EcoRI.. Agarose gel electrophoresis produced over 100 fragments, with fragme[r] ... See full document
6
Optimization of Computer Software Settings Improves Accuracy of Pulsed Field Gel Electrophoresis Macrorestriction Fragment Pattern Analysis
... of gel plug lot-to-lot variation was detected in gels 1 and 2; thus, plug lot did not contribute to the intragel variation found within either ...VBSs produced in lot-specific, intergel ...visual ... See full document
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Temperate Bacteriophages Affect Pulsed Field Gel Electrophoresis Patterns of Campylobacter jejuni
... amplified fragment length polymorphism analysis, randomly amplified polymorphic DNA typing, multi- plex PCR, pulsed-field gel electrophoresis (PFGE), and nu- cleic acid sequencing ... See full document
6
Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia
... All the strains were susceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetra- cycline, trimethoprim, gentamicin and co-trimoxazole. All the DNA samples could be restricted by PstI and ribotyping was ... See full document
5
Comparative Analysis of Infrequent Restriction Site PCR and Pulsed Field Gel Electrophoresis for Epidemiological Typing ofLegionella pneumophila Serogroup 1 Strains
... IRS-PCR produced isolate profiles that were concordant between those from the patients and the sources of ...on interpretation of PFGE and IRS-PCR were based principally on the guidelines described by ... See full document
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Pulsed Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains
... PFGE patterns for several bacterial species (4, 7, 18, 25, ...were produced with AscI and NotI ...the interpretation of DNA restriction patterns generated by PFGE described above, ... See full document
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Interpreting chromosomal DNA restriction patterns produced by pulsed field gel electrophoresis: criteria for bacterial strain typing
... DNA fragment patterns generated by PFGE and transform them into epidemiologically useful infor- mation, the microbiologist must understand how to compare PFGE patterns and how random genetic events ... See full document
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Performance Assessment of DNA Fragment Sizing by High Sensitivity Flow Cytometry and Pulsed Field Gel Electrophoresis
... Because macrorestriction fingerprints are commonly used to create phylogenetic comparisons in the form of dendrograms, we also chose to evaluate the precision of FCM and PFGE by using a dendrogram-derived method. ... See full document
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Subtyping of Haemophilus influenzaeStrains by Pulsed Field Gel Electrophoresis
... 4). Pulsed-field gel electrophoresis (PFGE) was performed in ...electric field appara- tus (CHEF-DR II apparatus; Bio-Rad, Richmond, ...agarose gel. Electrophoresis was ... See full document
6
Comparison of Fingerprinting Methods for Typing Methicillin Resistant Staphylococcus aureus Sequence Type 398
... single restriction enzyme (D ⫽ ...the restriction enzymes BstZI and ApaI was as high as the DI of PFGE using all three restriction enzymes ...using restriction enzyme SacII with 32 MRSA ST398 ... See full document
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Genetic Relationship between Methicillin Sensitive and Methicillin Resistant Staphylococcus aureus Strains from France and from International Sources: Delineation of Genomic Groups
... Bacteriophage typing used to be the reference method for epidemiological typing for S. aureus, but because of problems with reproducibility and the nontypeability of strains, it was replaced by molecular typing methods, ... See full document
6
Diversity of Pulsed Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates
... 1% pulsed-field- certified agarose (Bio-Rad) in ...and electrophoresis was carried out in a CHEF-DR III system (Bio-Rad) at 14°C for 21 h at 6 V/cm with a switch time of ...PFGE gel images ... See full document
7
Subspecies typing of Vibrio parahaemolyticus by pulsed field gel electrophoresis
... FIG. 3. Dendrogram showing the clustering of PFGE patterns (SfiI) for the V. parahaemolyticus isolates and strains listed in Table 1. The dendrogram was based on the squared Euclidean distance measure and average ... See full document
5
Development of a Multiplex PCR Based Rapid Typing Method for Enterohemorrhagic Escherichia coli O157 Strains
... agarose gel electrophoresis systems), the results can easily be transformed into digital data, and the genes for the major virulence markers of EHEC O157 (the stx 1 , stx 2 , and eae genes) can be detected ... See full document
7
Comparison of Pulsed Field Gel Electrophoresis and Coagulase Gene Restriction Profile Analysis Techniques in the Molecular Typing of Staphylococcus aureus
... Enterotoxins produced by the bacteria are believed to be wholly responsible for the symptoms of food poisoning (3); therefore, only enterotoxigenic strains of ... See full document
5
A unified approach to molecular epidemiology investigations: tools and patterns in California as a case study for endemic shigellosis
... ance patterns are influenced by many factors including geographic location, year in which the isolate was obtained, class of antimicrobial agent, pressure exerted by antimicrobial use, and isolate source ... See full document
11
Use of restriction fragment length polymorphisms resolved by pulsed field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes
... Mycobacterial strains from the Mycobacterium avium complex were compared with each other and with Mycobacterium phlei isolates by restriction endonuclease digestion of chromosomal DNA wi[r] ... See full document
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