[PDF] Top 20 Expression of the Antisense-to-Latency Transcript Long Noncoding RNA in Kaposi's Sarcoma-Associated Herpesvirus
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Expression of the Antisense-to-Latency Transcript Long Noncoding RNA in Kaposi's Sarcoma-Associated Herpesvirus
... Of the more than a dozen potential lncRNAs in the KSHV genome that have been proposed on the basis of transcriptome-wide studies, most were detected in lytically reactivated cells. Many are not consistently detected ... See full document
18
Making Sense of Antisense: Seemingly Noncoding RNAs Antisense to the Master Regulator of Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Do Not Regulate That Transcript but Serve as mRNAs Encoding Small Peptides
... putative noncoding RNAs, many of which are antisense to known open reading frames ...these antisense transcripts, but few have been experimentally examined, and such functions remain largely ... See full document
11
The Lytic Transcriptome of Kaposi's Sarcoma-Associated Herpesvirus Reveals Extensive Transcription of Noncoding Regions, Including Regions Antisense to Important Genes
... total RNA was labeled using the Eber- wine protocol, resulting in Cy5 (or Cy3)-labeled cRNA ...gene expression initially, but by 2 days postinfection, substantial levels of expression were observed ... See full document
9
The latency associated nuclear antigen of Kaposi sarcoma–associated herpesvirus induces B cell hyperplasia and lymphoma
... ectopic expression in B cell lines rendered cells resistant to p16INK4- mediated cell-cycle arrest ...The long latency to lymphoma development in our transgenic mice indicates that additional signals ... See full document
9
DNA Binding and Modulation of Gene Expression by the Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus
... The 801-bp TR unit of KSHV, defined by a unique NotI site, is highly repetitive and GC rich. We therefore prepared all probes by conventional cloning without the use of PCR ampli- fication steps. Plasmids pML1, ... See full document
11
Modulation of Cellular and Viral Gene Expression by the Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus
... gene expression from its targets remains a subject for further ...total RNA based on loading quantities shown in ...Staf-50 expression in two KS skin ...total RNA was loaded in each lane. The ... See full document
11
Kaposi's Sarcoma-Associated Herpesvirus K8 Is an RNA Binding Protein That Regulates Viral DNA Replication in Coordination with a Noncoding RNA
... T1.4 RNA in regulating KSHV DNA replication during de novo ...as noncoding regions is actu- ally transcribed and gives rise to stable ...of noncoding RNAs suggests that functional RNA-protein ... See full document
24
Interplay between Polyadenylate-Binding Protein 1 and Kaposi's Sarcoma-Associated Herpesvirus ORF57 in Accumulation of Polyadenylated Nuclear RNA, a Viral Long Noncoding RNA
... PAN RNA re- mains ...performed RNA pulldown assays using a PAN RNA oligomer, oJM35, that covers the MRE 9-nt core region in MRE stem-loop II FIG 2 KSHV ORF57 interacts with ... See full document
14
CTCF Regulates Kaposi's Sarcoma-Associated Herpesvirus Latency Transcription by Nucleosome Displacement and RNA Polymerase Programming
... affected latency transcription or spliced mRNA products, we investigated the rel- ative levels of formation of the various latency transcripts and their spliced products using exon-intron junction- and ... See full document
12
The Latency-Associated Nuclear Antigen of Kaposi's Sarcoma-Associated Herpesvirus Inhibits Expression of SUMO/Sentrin-Specific Peptidase 6 To Facilitate Establishment of Latency
... Kaposi’s sarcoma-associated herpesvirus (KSHV), which belongs to the Gammaherpesviridae, typically displays two different phases in its life cycle, the la- tent phase and the lytic ...phase. ... See full document
17
Establishment and Maintenance of Kaposi's Sarcoma-Associated Herpesvirus Latency in B Cells
... gene expression in B ...gene expression patterns as PEL cells, Northern blot analysis was done to probe for the expression of representative latent and lytic ...Polyadenylated RNA was isolated ... See full document
9
Kinetics of Kaposi’s Sarcoma-Associated Herpesvirus Gene Expression
... a transcript expressed in the majority of cells in KS biopsies, was considered to be a latent product, whereas ...[PAN] RNA or nut-1 RNA) was expressed in only a few cells in KS biopsies (51, 47, ... See full document
11
Regulation of Viral and Cellular Gene Expression by Kaposi's Sarcoma-Associated Herpesvirus Polyadenylated Nuclear RNA
... cellular RNA was harvested and the transcriptome for PAN RNA-expressing cells and control cells was elucidated (RNA-Seq) using next-generation sequencing (HiSeq 2000; 100 million reads for each ...in ... See full document
15
Murine Gammaherpesvirus 68 Expressing Kaposi Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen (LANA) Reveals both Functional Conservation and Divergence in LANA Homologs
... transgenic expression of KSHV LANA in mice promotes the development of lymphoid hyperplasia and lymphoma ...establish latency in mice following intranasal ...during latency is conserved among LANA ... See full document
13
Reduction of Kaposi’s Sarcoma-Associated Herpesvirus Latency Using CRISPR-Cas9 To Edit the Latency-Associated Nuclear Antigen Gene
... mRNA expression of a targeted gene should not be directly impacted, and any decrease in mRNA would be a result of the decrease in its template DNA ...mRNA expression slightly in- creased after adenovirus ... See full document
17
Kaposi's Sarcoma-Associated Herpesvirus Noncoding Polyadenylated Nuclear RNA Interacts with Virus- and Host Cell-Encoded Proteins and Suppresses Expression of Genes Involved in Immune Modulation
... PAN RNA binding ...PAN RNA in KSHV growth, we wanted to develop a protocol to identify putative factors that bind to PAN RNA in infected ...other noncoding RNAs were elucidated using an ... See full document
8
Identification of the gene encoding the major latency associated nuclear antigen of the Kaposi's sarcoma associated herpesvirus
... prepared RNA from BCBL-1 cells grown in the presence or absence of TPA and examined this RNA for viral gene expression by Northern blotting, using as probes a number of large viral DNA fragments from ... See full document
6
Cloning and Identification of a MicroRNA Cluster within the Latency-Associated Region of Kaposi's Sarcoma-Associated Herpesvirus
... unique RNA species between 19 and 24 bases in length matched with 100% comple- mentarity to ...the latency-associated locus (11, 27). During latency, KSHV gene expression is re- ... See full document
5
Induction of Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen by the Lytic Transactivator RTA: a Novel Mechanism for Establishment of Latency
... RTA transcript levels began to level off from a C t value of about 30 to approximately ...LANA transcript levels is likely due to a lag between increased transcription of RTA and the overall change in LANA ... See full document
13
Long-Term-Infected Telomerase-Immortalized Endothelial Cells: a Model for Kaposi's Sarcoma-Associated Herpesvirus Latency In Vitro and In Vivo
... KSHV latency in the absence of selection and that underwent marked phe- notypical ...changes. Long-term-infected TIVE cells (LTC), but not uninfected TIVE cells, formed colonies in soft agar and efficiently ... See full document
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