[PDF] Top 20 Expression in COS-1 cells of Epstein-Barr virus nuclear antigen from a complete gene and a deleted gene.

... Sources of antibody were human sera which were characterized with respect to their content of antibody to early antigen, capsid antigen, and EBNA; their reactivity to the nuclear antigen[r] ... See full document

... 324-330 0022-538X/86/050324-07$02.00/0 Copyright X 1986, American Society for Microbiology Deletion Mutants That Affect Expression of Epstein-Barr Virus Nuclear Antigen in COS-1 Cells af[r] ... See full document

... surface antigen, cytokine expression, and tumorigenicity in the HD cell line ...the expression of many different sur- face antigens such as CD30, CD39, CD40, CD44, CD54 (in- tercellular adhesion ... See full document

... B cells in vitro (13). EBNA2 is a key regulator of LMP1 expression, and EBNA2 activity is largely under viral control and at the level of the CBF1 ...(Fig. 1), the elevated levels of bfl-1 ... See full document

... c-myc expression, drives cell growth but also sensitizes the cells to apoptotic signals ...EBV gene expression in Wp-restricted tumors con- ferred even further resistance to ...EBNA2 ... See full document

... LMP-1 gene expression underscores the complex nature of LMP-1 gene ...LMP-1 expression in a cell cycle-dependent manner. LMP-1 is clearly an important protein in ... See full document

... When Raji cells are allowed to grow to high density and most of the cells become growth arrested in the G, phase of the cell cycle, the level of detectable latent membrane protein 1 LMP1[r] ... See full document

... of nuclear hormone receptors (4, 51, 64, 72); it controls the cell cycle and promotes prolif- eration of tumor cells (71, 74), and it is a member of the class E vacuolar protein-sorting (vps) family and ... See full document

... to Cp promoter-driven EBNA synthesis and also regulates synthesis of the LMP1 and LMP2 latency membrane proteins. However, EBNA2 is detected in EBV-associated malignancies only in the context of immunosuppression, ... See full document

... cancer cells origi- nated from different human organizations, including gastric [13], pancreas [14–16], brain [17], colon [18], prostate [19], blood [20], breast [21, 22], and ovary ...downstream ... See full document

... FIG 1 EBNALP drastically enhances the transcription activity of EP300 tethered to a promoter and binds to ...BJAB cells were cotransfected with the following constructs: the pE2Luciferase reporter, which ... See full document

... SM295 cells and could not been ...and expression is repressed by ...ensemble-predicted gene that has not yet been functionally analyzed in ...drawn from our results is that CBF1 is the major ... See full document

... other gene (p554) encodes a protein that is not demonstrably cross-reactive with Epstein-Barr virus nuclear antigen-1 or with any other EBV protein, but forms complexes ... See full document

... B95.8 cells, of which ca. 1 to 5% are lytically ...BL cells (located ...Akata cells, while detecting Fp-initiated tran- scripts only in the undiluted RNA ... See full document

... the gene template. Notably, reporter constructs in which EBNA-1 binding sites are placed as little as an additional 40 bp downstream from the transcription start site, albeit in a heterologous ... See full document

... (A) Cells from two HH514- NoER clones, two BL41-P3HR1-NoER clones, and the parental cell lines were transiently transfected with an LMP1 promoter-luciferase ...derived from BL41-P3HR1 cells ... See full document

... In this report, we describe the identification of the ORFs on the EBV genome encoding the p18 and p40 proteins, using a monoclonal antibody MAb to VCA-p40 and VCAp18 affinity-purified po[r] ... See full document

... The probe was hybridized to tRNA lane 2 as a negative control, RNA from untreated Raji cells lane 3, RNA from Raji cells 4, 8, and 12 h after superinfection with P3HR1 EBV lanes 4, 5, an[r] ... See full document

... We investigated EBV gene expression in purified uncultured PBLs from normal, healthy seropositive adults by using RNA polymerase chain reaction PCR to amplify the minute quantities of vi[r] ... See full document

... away from the ribosome to a perinuclear compartment re- ferred to as the GW body (26, 27, ...microRNA expression profiles and specific cancer lineages have been ob- served (for reviews, see references 7 and ... See full document