[PDF] Top 20 Formation of nsP3-Specific Protein Complexes during Sindbis Virus Replication
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Formation of nsP3-Specific Protein Complexes during Sindbis Virus Replication
... of nsP3/GFP expres- sion and distribution in the infected ...of nsP3/GFP was distributed in the cells in a diffuse, nonaggregated smear-like form but some of the pro- tein had already formed larger ... See full document
13
Role for the Phosphatidylinositol 3-Kinase-Akt-TOR Pathway during Sindbis Virus Replication in Arthropods
... the virus-arthropod host interaction is not well ...viral replication; conversely, repression can enhance cell ...study Sindbis virus RNA replication in Drosophila melanogaster, we ... See full document
10
A New Role for ns Polyprotein Cleavage in Sindbis Virus Replication
... the formation of the viral replication complex (RC), which func- tions in both RNA genome replication and the synthesis of the subgenomic (SG) ...prototypes, Sindbis virus (SINV) and ... See full document
14
Hypervariable Domain of Nonstructural Protein nsP3 of Venezuelan Equine Encephalitis Virus Determines Cell-Specific Mode of Virus Replication
... to virus trans- mission to vertebrates during the next blood ...the virus to new mosquito vectors ...alitis virus (VEEV), eastern equine encephalitis virus (EEEV), and western equine ... See full document
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Multiple Host Factors Interact with the Hypervariable Domain of Chikungunya Virus nsP3 and Determine Viral Replication in Cell-Specific Mode
... viral replication in mosquito, but not in vertebrate BHK-21 or NIH 3T3, cells is represented by the proline-rich motif ...BIN1 protein (44). The latter protein contains both Bin-Amphiphysin- Rus ... See full document
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Functional Sindbis Virus Replicative Complexes Are Formed at the Plasma Membrane
... RC formation and function have been intensively studied using the SINV and SFV models (5, 13, 15, 22, 23, ...(15). Replication of viral RNA coincides with the appearance of numerous membrane spher- ules ... See full document
17
Induction of Apoptosis by Sindbis Virus Occurs at Cell Entry and Does Not Require Virus Replication
... the virus, while others act intracellularly (4, 33, ...capsid protein and two surface gly- coproteins, E1 and E2 ...the virus to cells to initiate ...and formation of membrane-bound ... See full document
7
Multiple roles of the non-structural protein 3 (nsP3) alphavirus unique domain (AUD) during Chikungunya virus genome replication and transcription
... Although nsP3 plays an essential role in RNA replication, its bio- chemical functions remain largely undefined ...Rubella virus (the sole member of the Rubivirus genus within the Togaviridae), and a ... See full document
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Hypervariable Domains of nsP3 Proteins of New World and Old World Alphaviruses Mediate Formation of Distinct, Virus-Specific Protein Complexes
... in nsP3 protein have no negative ef- fect on VEEV and SINV ...into virus-specific proteins provides an opportunity to investigate their intracellular compartmentalization and temporal-spatial ... See full document
14
Different Types of nsP3-Containing Protein Complexes in Sindbis Virus-Infected Cells
... of virus-specific RNAs and in modification of the intracellular ...that Sindbis virus (SINV) infection in BHK-21 cells leads to the formation of at least two types of ... See full document
14
Role of glycoprotein PE2 in formation and maturation of the Sindbis virus spike.
... Sindbis virus envelope assembly is a multistep process resulting in the maturation of a rigid, highly ordered T 5 4 icosahedral protein lattice containing 80 spikes composed of trimers of E1-E2 ... See full document
9
The p23 Protein of Hibiscus Chlorotic Ringspot Virus Is Indispensable for Host-Specific Replication
... ringspot virus (HCRSV) belongs to the Tombusviridae family of plant ...mottle virus (16), Turnip crinkle virus (TCV) (6), Melon necrotic spot virus (35), Cardamine chlorotic fleck virus ... See full document
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Sindbis Virus Infection Causes Cell Death by nsP2-Induced Transcriptional Shutoff or by nsP3-Dependent Translational Shutoff
... Characterization of viruses with selected mutations. In this study, we focused on three selected mutations, namely, H619Q, H643Q, and P683Q, that were located in the protease domain and were predicted to be at least ... See full document
20
Serine phosphorylation of the hepatitis C virus NS5A protein controls the establishment of replication complexes
... SDS-PAGE/Western blotting. Cells were washed twice in PBS, lysed in 1⫻ Glasgow lysis buffer (GLB; 1% [vol/vol] Triton X-100, 120 mM KCl, 30 mM NaCl, 5 mM MgCl 2 , 10% [vol/vol] glycerol, 10 mM PIPES [piperazine-N,N = ... See full document
14
Serine Phosphorylation of the Hepatitis C Virus NS5A Protein Controls the Establishment of Replication Complexes
... C virus (HCV) nonstructural 5A (NS5A) protein is highly phosphorylated and involved in both virus genome repli- cation and virion ...the virus life cycle remains ...infectious virus and ... See full document
13
Adeno-Associated Virus Type 2 Protein Interactions: Formation of Pre-Encapsidation Complexes
... Rep protein concentration present in the cell extract ...Rep-VP complexes under less stringent conditions, cell extracts prepared using detergent-free buffer A were used for immunoprecipitation with the VP ... See full document
10
Polyprotein-Driven Formation of Two Interdependent Sets of Complexes Supporting Hepatitis C Virus Genome Replication
... the replication complex ...the replication complex included S2208I and P2008A; these mutations disrupted NS3-5A- and NS3-5B-dependent complex activity, respectively, in our ...within complexes occurs ... See full document
16
Cellular Vimentin Regulates Construction of Dengue Virus Replication Complexes through Interaction with NS4A Protein
... vimentin during DENV ...the virus-induced ER-derived membranous compart- ments serve as a scaffold for the DENV RCs and NS4A associates with ER membranes, we analyzed the colocalization of DENV RCs with ... See full document
17
Role of ATM in the Formation of the Replication Compartment during Lytic Replication of Epstein-Barr Virus in Nasopharyngeal Epithelial Cells
... viral replication compartment during lytic EBV ...29–31). During lytic cycle induction in EBV-infected B cells, the ATM and MRN subunits were shown to be localized at the viral replication ... See full document
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Comparison of Membrane Protein Glycopeptides of Sindbis Virus and Vesicular Stomatitis Virus
... Although glycopeptides of membrane proteins of chicken and hamster cells cover a spectrum of molecular weights from 1,000 daltons to more than 6,000 daltons 1, the glycopeptides of Sindb[r] ... See full document
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