[PDF] Top 20 Functional analysis of the herpes simplex virus UL42 protein.

... This altered polypeptide also failed to increase the length of DNA product synthesized by Pol, and the mutant gene could not complement the growth of a UL42 null virus, indicating that t[r] ... See full document

... does UL42 increase processivity? Current and previous results support the model that UL42 acts as a tether between Pol and DNA by virtue of its ability to bind both the catalytic subunit and the DNA ... See full document

... the protein for early degradation, similar to the situation that occurs with ...the protein for immediate degrada- ...the protein is often seen in the im- munoprecipitated complex ...truncated ... See full document

... of virus assembly and egress has been the inability to analyze and follow virus traf- ficking at the single-cell ...of protein trafficking in several aspects of cell biology, such as endoplasmic ... See full document

... parental virus used for the construction of the AcUL28Myc baculovirus was AcPAK6 ...tagged protein was cloned into the SmaI site of the transfer vector pAcCL29-1 (20) in the correct orientation downstream ... See full document

... cellular protein phosphatase 1 to dephosphorylate the ␣ subunit of elongation initiator factor 2 that has been phosphor- ylated by the activated protein kinase R (7, ... See full document

... recombinant virus, ⌬ 20/gKD3prtC, was constructed by replacing the UL20 gene of the recombinant virus gKD3prtC (17) with a gene cassette expressing the EGFP gene under control of the cytomegalovirus ... See full document

... major functional consequences of HSV-1 Us3 kinase activity is to promote nu- clear egress of nucleocapsids, probably by regulating proper localization of nuclear egress factors UL31 and UL34 at the nuclear ... See full document

... each virus, although significant details may vary and some homologs may have additional functions such as attach- ...of herpes simplex virus 1 (HSV-1) and HSV-2, which share an 87% sequence ... See full document

... roller bottles of confluent Vero cells were infected at an MOI of 10. At 16 to 20 h postinfection, cells were scraped into 20 ml of PBS, collected by centrifugation at 1,000 ⫻ g for 10 min, resuspended in 6 ml of NP-40 ... See full document

... been reported recently (4), although reagents capable of de- tecting expression of U S 1.5 using these procedures were not available. In order to determine if U S 1.5 can be expressed in transient assays in the presence ... See full document

... The herpes simplex virus 1 (HSV-1) UL12 protein (pUL12) is a nuclease that is critical for viral replication in vitro and neuro- virulence in ...spectrometric analysis of pUL12 and ... See full document

... Importantly, the results allow the separation of requirements for functional activity from those for complex formation, since deletion mutant pRG15, containing 434 amino acids, promoted [r] ... See full document

... In electron micrographs, cytoplasmic VLVs were sur- rounded by an additional membrane (Fig. 2Dg and h). The origin of the outer membrane is unclear, but the following observations lead us to hypothesize that the membrane ... See full document

... Mutational analysis of UL25 ...UL25 protein consists of numerous flexible loops extend- ing from a rigid core composed of several tightly packed alpha ...the protein and hence inhibit its ...folded ... See full document

... affect protein synthesis prior to 4 ...cells, protein synthesis inhibition coincides with the onset of viral DNA replication (10, ...the functional data obtained at 3 hpi suggest a rapid antiviral ... See full document

... of herpes simplex virus type 1 (HSV-1) is a stable heterodimer consisting of a catalytic subunit (Pol) and a processivity factor ...HSV-1 UL42 differs from most DNA polymerase processivity ... See full document

... the functional NLS is contained within the first 56 amino acids of VP19C, the function of the rest of the poorly conserved N-terminal region remains ...scopic analysis failed to identify capsid formation by ... See full document

... Although a UL42 mutant RNA encoding a product lacking the 28 C-terminal residues resulted in wildtype levels of pol stimulation, the failure of large aminoterminal N-terminal and C-termi[r] ... See full document

... Quantitative analysis of the interaction between the DNA polymerase subunits and HP96 by the gel mobility shift assay showed that in addition to altering the details of the nuclease prot[r] ... See full document